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Genotype Distribution and Molecular Epidemiology of Hepatitis C Virus in Hubei, Central China.

Peng J, Lu Y, Liu W, Zhu Y, Yan X, Xu J, Wang X, Wang Y, Liu W, Sun Z - PLoS ONE (2015)

Bottom Line: Analysis of genotype-associated risk factors revealed that paid blood donation and transfusion before 1997 were strongly associated with subtypes 1b and 2a, while some subtype 2a cases were also found in individuals with high risk sexual behaviors; subtypes 3b, 6a, and 3a were detected only in intravenous drug users.Among them, subtype 1b Hubei strains may have served as the origins of this subtype in China, and 2a and 3b Hubei strains may have descended from the northwest and southwest of China, respectively, while 6a Hubei strains may have been imported from the central south and southwest.The results suggest that the migration patterns of HCV in Hubei are complex and variable among different subtypes.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Laboratory, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

ABSTRACT

Background: Little is known about the molecular epidemiology of hepatitis C virus (HCV) infection in Central China.

Methodology/principal findings: A total of 570 patients from Hubei Province in central China were enrolled. These patients were tested positive for HCV antibody prior to blood transfusion. Among them, 177 were characterized by partial NS5B and/or Core-E1 sequences and classified into five subtypes: 1b, 83.0% (147/177); 2a, 13.0% (23/177); 3b, 2.3% (4/177); 6a, 1.1% (2/177); 3a, 0.6% (1/177). Analysis of genotype-associated risk factors revealed that paid blood donation and transfusion before 1997 were strongly associated with subtypes 1b and 2a, while some subtype 2a cases were also found in individuals with high risk sexual behaviors; subtypes 3b, 6a, and 3a were detected only in intravenous drug users. Phylogeographic analyses based on the coalescent datasets demonstrated that 1b, 2a, 3b, and 6a were locally epidemic in Hubei Province. Among them, subtype 1b Hubei strains may have served as the origins of this subtype in China, and 2a and 3b Hubei strains may have descended from the northwest and southwest of China, respectively, while 6a Hubei strains may have been imported from the central south and southwest.

Conclusion/significance: The results suggest that the migration patterns of HCV in Hubei are complex and variable among different subtypes. Implementation of mandatory HCV screening before donation has significantly decreased the incidence of transfusion-associated HCV infection since 1997. More attention should be paid to intravenous drug use and unsafe sexual contact, which may have become new risk factors for HCV infection in Hubei Province.

No MeSH data available.


Related in: MedlinePlus

Phylogeographic tree generated with the partial Core-E1 sequences of subtype 3b isolates.A total of 68 subtype 3b sequences were used to estimate the phylogeographic tree: 4 obtained in this study, 20 from IDUs in Wuhan City [14], and 44 from blood donors from the 17 provinces and municipalities in China [5]. The indications here are the same as those in Fig 3.
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pone.0137059.g005: Phylogeographic tree generated with the partial Core-E1 sequences of subtype 3b isolates.A total of 68 subtype 3b sequences were used to estimate the phylogeographic tree: 4 obtained in this study, 20 from IDUs in Wuhan City [14], and 44 from blood donors from the 17 provinces and municipalities in China [5]. The indications here are the same as those in Fig 3.

Mentions: Fig 5 displays a phylogeographic tree for 68 subtype 3b sequences. As a whole, four groups were visible, but only the lowest one showed significant posterior probability of 0.71 and this group featured sequences mainly from the southwest, in particular, Yunnan Province. The upper group contained sequences mostly from Hubei except for one from the northwest, suggesting a local epidemic in this area. The two middle groups showed substantial geographic interspersion. As regards migration routes, 3b in China may have originated from Yunnan in the southwest, whereupon spread to other regions.


Genotype Distribution and Molecular Epidemiology of Hepatitis C Virus in Hubei, Central China.

Peng J, Lu Y, Liu W, Zhu Y, Yan X, Xu J, Wang X, Wang Y, Liu W, Sun Z - PLoS ONE (2015)

Phylogeographic tree generated with the partial Core-E1 sequences of subtype 3b isolates.A total of 68 subtype 3b sequences were used to estimate the phylogeographic tree: 4 obtained in this study, 20 from IDUs in Wuhan City [14], and 44 from blood donors from the 17 provinces and municipalities in China [5]. The indications here are the same as those in Fig 3.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556612&req=5

pone.0137059.g005: Phylogeographic tree generated with the partial Core-E1 sequences of subtype 3b isolates.A total of 68 subtype 3b sequences were used to estimate the phylogeographic tree: 4 obtained in this study, 20 from IDUs in Wuhan City [14], and 44 from blood donors from the 17 provinces and municipalities in China [5]. The indications here are the same as those in Fig 3.
Mentions: Fig 5 displays a phylogeographic tree for 68 subtype 3b sequences. As a whole, four groups were visible, but only the lowest one showed significant posterior probability of 0.71 and this group featured sequences mainly from the southwest, in particular, Yunnan Province. The upper group contained sequences mostly from Hubei except for one from the northwest, suggesting a local epidemic in this area. The two middle groups showed substantial geographic interspersion. As regards migration routes, 3b in China may have originated from Yunnan in the southwest, whereupon spread to other regions.

Bottom Line: Analysis of genotype-associated risk factors revealed that paid blood donation and transfusion before 1997 were strongly associated with subtypes 1b and 2a, while some subtype 2a cases were also found in individuals with high risk sexual behaviors; subtypes 3b, 6a, and 3a were detected only in intravenous drug users.Among them, subtype 1b Hubei strains may have served as the origins of this subtype in China, and 2a and 3b Hubei strains may have descended from the northwest and southwest of China, respectively, while 6a Hubei strains may have been imported from the central south and southwest.The results suggest that the migration patterns of HCV in Hubei are complex and variable among different subtypes.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Laboratory, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

ABSTRACT

Background: Little is known about the molecular epidemiology of hepatitis C virus (HCV) infection in Central China.

Methodology/principal findings: A total of 570 patients from Hubei Province in central China were enrolled. These patients were tested positive for HCV antibody prior to blood transfusion. Among them, 177 were characterized by partial NS5B and/or Core-E1 sequences and classified into five subtypes: 1b, 83.0% (147/177); 2a, 13.0% (23/177); 3b, 2.3% (4/177); 6a, 1.1% (2/177); 3a, 0.6% (1/177). Analysis of genotype-associated risk factors revealed that paid blood donation and transfusion before 1997 were strongly associated with subtypes 1b and 2a, while some subtype 2a cases were also found in individuals with high risk sexual behaviors; subtypes 3b, 6a, and 3a were detected only in intravenous drug users. Phylogeographic analyses based on the coalescent datasets demonstrated that 1b, 2a, 3b, and 6a were locally epidemic in Hubei Province. Among them, subtype 1b Hubei strains may have served as the origins of this subtype in China, and 2a and 3b Hubei strains may have descended from the northwest and southwest of China, respectively, while 6a Hubei strains may have been imported from the central south and southwest.

Conclusion/significance: The results suggest that the migration patterns of HCV in Hubei are complex and variable among different subtypes. Implementation of mandatory HCV screening before donation has significantly decreased the incidence of transfusion-associated HCV infection since 1997. More attention should be paid to intravenous drug use and unsafe sexual contact, which may have become new risk factors for HCV infection in Hubei Province.

No MeSH data available.


Related in: MedlinePlus