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Genotype Distribution and Molecular Epidemiology of Hepatitis C Virus in Hubei, Central China.

Peng J, Lu Y, Liu W, Zhu Y, Yan X, Xu J, Wang X, Wang Y, Liu W, Sun Z - PLoS ONE (2015)

Bottom Line: Analysis of genotype-associated risk factors revealed that paid blood donation and transfusion before 1997 were strongly associated with subtypes 1b and 2a, while some subtype 2a cases were also found in individuals with high risk sexual behaviors; subtypes 3b, 6a, and 3a were detected only in intravenous drug users.Among them, subtype 1b Hubei strains may have served as the origins of this subtype in China, and 2a and 3b Hubei strains may have descended from the northwest and southwest of China, respectively, while 6a Hubei strains may have been imported from the central south and southwest.The results suggest that the migration patterns of HCV in Hubei are complex and variable among different subtypes.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Laboratory, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

ABSTRACT

Background: Little is known about the molecular epidemiology of hepatitis C virus (HCV) infection in Central China.

Methodology/principal findings: A total of 570 patients from Hubei Province in central China were enrolled. These patients were tested positive for HCV antibody prior to blood transfusion. Among them, 177 were characterized by partial NS5B and/or Core-E1 sequences and classified into five subtypes: 1b, 83.0% (147/177); 2a, 13.0% (23/177); 3b, 2.3% (4/177); 6a, 1.1% (2/177); 3a, 0.6% (1/177). Analysis of genotype-associated risk factors revealed that paid blood donation and transfusion before 1997 were strongly associated with subtypes 1b and 2a, while some subtype 2a cases were also found in individuals with high risk sexual behaviors; subtypes 3b, 6a, and 3a were detected only in intravenous drug users. Phylogeographic analyses based on the coalescent datasets demonstrated that 1b, 2a, 3b, and 6a were locally epidemic in Hubei Province. Among them, subtype 1b Hubei strains may have served as the origins of this subtype in China, and 2a and 3b Hubei strains may have descended from the northwest and southwest of China, respectively, while 6a Hubei strains may have been imported from the central south and southwest.

Conclusion/significance: The results suggest that the migration patterns of HCV in Hubei are complex and variable among different subtypes. Implementation of mandatory HCV screening before donation has significantly decreased the incidence of transfusion-associated HCV infection since 1997. More attention should be paid to intravenous drug use and unsafe sexual contact, which may have become new risk factors for HCV infection in Hubei Province.

No MeSH data available.


Related in: MedlinePlus

Phylogeographic tree generated with the partial Core-E1 sequences of subtype 2a isolates.A total of 106 subtype 2a sequences were used to generate the phylogeographic tree: 23 obtained in our study, 1 from a male homosexual in Wuhan City [14], and 81 from blood donors from the 17 provinces and municipalities in China, including 2 from Hubei [5]. The indications in this legend are the same as those in Fig 3.
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pone.0137059.g004: Phylogeographic tree generated with the partial Core-E1 sequences of subtype 2a isolates.A total of 106 subtype 2a sequences were used to generate the phylogeographic tree: 23 obtained in our study, 1 from a male homosexual in Wuhan City [14], and 81 from blood donors from the 17 provinces and municipalities in China, including 2 from Hubei [5]. The indications in this legend are the same as those in Fig 3.

Mentions: Fig 4 presents a phylogeographic tree for 106 subtype 2a sequences. Overall, these 2a sequences can be classified into three big groups, showing significant posterior probabilities of 0.99, 1.00, and 1.00, respectively. Among them, four loose groups were observed, but they showed no significant posterior probabilities. The upper group had the largest number size and contained sequences mainly from the northwest. The middle one had the majority of its sequences originating in Hubei, while the lower one displayed branches with sequences with a mixture of geographic origins. It seems that 2a in China may have descended from a common ancestor in the northwest dated around 1968 (95% CI: 1949–1984), from where it was transmitted to other regions and became locally epidemic in Hubei and the northwest. The same geographic distribution pattern and migration trend of 2a strains were also observed in the phylogeographic tree estimated with the NS5B sequences (S2 Fig), except that the upper group showed a posterior probability of 0.61.


Genotype Distribution and Molecular Epidemiology of Hepatitis C Virus in Hubei, Central China.

Peng J, Lu Y, Liu W, Zhu Y, Yan X, Xu J, Wang X, Wang Y, Liu W, Sun Z - PLoS ONE (2015)

Phylogeographic tree generated with the partial Core-E1 sequences of subtype 2a isolates.A total of 106 subtype 2a sequences were used to generate the phylogeographic tree: 23 obtained in our study, 1 from a male homosexual in Wuhan City [14], and 81 from blood donors from the 17 provinces and municipalities in China, including 2 from Hubei [5]. The indications in this legend are the same as those in Fig 3.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556612&req=5

pone.0137059.g004: Phylogeographic tree generated with the partial Core-E1 sequences of subtype 2a isolates.A total of 106 subtype 2a sequences were used to generate the phylogeographic tree: 23 obtained in our study, 1 from a male homosexual in Wuhan City [14], and 81 from blood donors from the 17 provinces and municipalities in China, including 2 from Hubei [5]. The indications in this legend are the same as those in Fig 3.
Mentions: Fig 4 presents a phylogeographic tree for 106 subtype 2a sequences. Overall, these 2a sequences can be classified into three big groups, showing significant posterior probabilities of 0.99, 1.00, and 1.00, respectively. Among them, four loose groups were observed, but they showed no significant posterior probabilities. The upper group had the largest number size and contained sequences mainly from the northwest. The middle one had the majority of its sequences originating in Hubei, while the lower one displayed branches with sequences with a mixture of geographic origins. It seems that 2a in China may have descended from a common ancestor in the northwest dated around 1968 (95% CI: 1949–1984), from where it was transmitted to other regions and became locally epidemic in Hubei and the northwest. The same geographic distribution pattern and migration trend of 2a strains were also observed in the phylogeographic tree estimated with the NS5B sequences (S2 Fig), except that the upper group showed a posterior probability of 0.61.

Bottom Line: Analysis of genotype-associated risk factors revealed that paid blood donation and transfusion before 1997 were strongly associated with subtypes 1b and 2a, while some subtype 2a cases were also found in individuals with high risk sexual behaviors; subtypes 3b, 6a, and 3a were detected only in intravenous drug users.Among them, subtype 1b Hubei strains may have served as the origins of this subtype in China, and 2a and 3b Hubei strains may have descended from the northwest and southwest of China, respectively, while 6a Hubei strains may have been imported from the central south and southwest.The results suggest that the migration patterns of HCV in Hubei are complex and variable among different subtypes.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Laboratory, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

ABSTRACT

Background: Little is known about the molecular epidemiology of hepatitis C virus (HCV) infection in Central China.

Methodology/principal findings: A total of 570 patients from Hubei Province in central China were enrolled. These patients were tested positive for HCV antibody prior to blood transfusion. Among them, 177 were characterized by partial NS5B and/or Core-E1 sequences and classified into five subtypes: 1b, 83.0% (147/177); 2a, 13.0% (23/177); 3b, 2.3% (4/177); 6a, 1.1% (2/177); 3a, 0.6% (1/177). Analysis of genotype-associated risk factors revealed that paid blood donation and transfusion before 1997 were strongly associated with subtypes 1b and 2a, while some subtype 2a cases were also found in individuals with high risk sexual behaviors; subtypes 3b, 6a, and 3a were detected only in intravenous drug users. Phylogeographic analyses based on the coalescent datasets demonstrated that 1b, 2a, 3b, and 6a were locally epidemic in Hubei Province. Among them, subtype 1b Hubei strains may have served as the origins of this subtype in China, and 2a and 3b Hubei strains may have descended from the northwest and southwest of China, respectively, while 6a Hubei strains may have been imported from the central south and southwest.

Conclusion/significance: The results suggest that the migration patterns of HCV in Hubei are complex and variable among different subtypes. Implementation of mandatory HCV screening before donation has significantly decreased the incidence of transfusion-associated HCV infection since 1997. More attention should be paid to intravenous drug use and unsafe sexual contact, which may have become new risk factors for HCV infection in Hubei Province.

No MeSH data available.


Related in: MedlinePlus