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Exploitation of the Medfly Gut Microbiota for the Enhancement of Sterile Insect Technique: Use of Enterobacter sp. in Larval Diet-Based Probiotic Applications.

Augustinos AA, Kyritsis GA, Papadopoulos NT, Abd-Alla AM, Cáceres C, Bourtzis K - PLoS ONE (2015)

Bottom Line: The Mediterranean fruit fly (medfly), Ceratitis capitata, is a pest of worldwide substantial economic importance, as well as a Tephritidae model for sterile insect technique (SIT) applications.Recent studies have suggested that the manipulation of gut symbionts can have a significant positive effect on the overall fitness of insect strains.Probiotic application of Enterobacter sp. resulted in improvement of both pupal and adult productivity, as well as reduced rearing duration, particularly for males, without affecting pupal weight, sex ratio, male mating competitiveness, flight ability and longevity under starvation.

View Article: PubMed Central - PubMed

Affiliation: Insect Pest Control Laboratory, Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, Seibersdorf, Vienna, Austria; Department of Environmental and Natural Resources Management, University of Patras, Agrinio, Greece.

ABSTRACT
The Mediterranean fruit fly (medfly), Ceratitis capitata, is a pest of worldwide substantial economic importance, as well as a Tephritidae model for sterile insect technique (SIT) applications. The latter is partially due to the development and utilization of genetic sexing strains (GSS) for this species, such as the Vienna 8 strain, which is currently used in mass rearing facilities worldwide. Improving the performance of such a strain both in mass rearing facilities and in the field could significantly enhance the efficacy of SIT and reduce operational costs. Recent studies have suggested that the manipulation of gut symbionts can have a significant positive effect on the overall fitness of insect strains. We used culture-based approaches to isolate and characterize gut-associated bacterial species of the Vienna 8 strain under mass rearing conditions. We also exploited one of the isolated bacterial species, Enterobacter sp., as dietary supplement (probiotic) to the larval diet, and we assessed its effects on fitness parameters under the standard operating procedures used in SIT operational programs. Probiotic application of Enterobacter sp. resulted in improvement of both pupal and adult productivity, as well as reduced rearing duration, particularly for males, without affecting pupal weight, sex ratio, male mating competitiveness, flight ability and longevity under starvation.

No MeSH data available.


Related in: MedlinePlus

Pupae (A) and adult (B) recovery rates.i) irrespective of the Enterobacter sp. concentration, or ii) considering the three different Enterobacter sp. concentrations as different treatments. Columns marked on top with the same letter are not significantly different (P>0.05)
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pone.0136459.g002: Pupae (A) and adult (B) recovery rates.i) irrespective of the Enterobacter sp. concentration, or ii) considering the three different Enterobacter sp. concentrations as different treatments. Columns marked on top with the same letter are not significantly different (P>0.05)

Mentions: As stated above, logistic regression analysis revealed that the bacterial concentration (106, 107 or 108 bacteria per gram of larval diet) was not a significant predictor of egg to pupa recovery rates (Wald’s test t = 1.01, df = 2, P = 0.603). Similar results were obtained when “autoclaved” (Wald’s test t = 0.13, df = 1, P = 0.715) and “live” bacteria (Wald’s test t = 0.06, df = 1, P = 0.802) were analyzed independently. In Fig 2, we combined the data for three different concentrations of “autoclaved bacteria” and the three concentrations of “live bacteria” diet, thus summarizing three discrete treatments (“without bacteria”, “autoclaved bacteria”, and “live bacteria”). The provision of Enterobacter sp. was a significant predictor of the pupae recovery rate (Wald’s test t = 9.97, df = 2, P = 0.007). The addition of “live bacteria” in the diet increased the pupal recovery rate over both control and autoclaved bacteria (Wald’s test t = 8.98, df = 1, P = 0.003, and t = 3.99, df = 1, P = 0.046 respectively). Interestingly, although the addition of “autoclaved bacteria” seems to lead also to an increased pupae recovery compared to “without bacteria” treatment, this difference was not significant (Wald’s test t = 2.499, df = 1, P = 0.114) (Fig 2A).


Exploitation of the Medfly Gut Microbiota for the Enhancement of Sterile Insect Technique: Use of Enterobacter sp. in Larval Diet-Based Probiotic Applications.

Augustinos AA, Kyritsis GA, Papadopoulos NT, Abd-Alla AM, Cáceres C, Bourtzis K - PLoS ONE (2015)

Pupae (A) and adult (B) recovery rates.i) irrespective of the Enterobacter sp. concentration, or ii) considering the three different Enterobacter sp. concentrations as different treatments. Columns marked on top with the same letter are not significantly different (P>0.05)
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556606&req=5

pone.0136459.g002: Pupae (A) and adult (B) recovery rates.i) irrespective of the Enterobacter sp. concentration, or ii) considering the three different Enterobacter sp. concentrations as different treatments. Columns marked on top with the same letter are not significantly different (P>0.05)
Mentions: As stated above, logistic regression analysis revealed that the bacterial concentration (106, 107 or 108 bacteria per gram of larval diet) was not a significant predictor of egg to pupa recovery rates (Wald’s test t = 1.01, df = 2, P = 0.603). Similar results were obtained when “autoclaved” (Wald’s test t = 0.13, df = 1, P = 0.715) and “live” bacteria (Wald’s test t = 0.06, df = 1, P = 0.802) were analyzed independently. In Fig 2, we combined the data for three different concentrations of “autoclaved bacteria” and the three concentrations of “live bacteria” diet, thus summarizing three discrete treatments (“without bacteria”, “autoclaved bacteria”, and “live bacteria”). The provision of Enterobacter sp. was a significant predictor of the pupae recovery rate (Wald’s test t = 9.97, df = 2, P = 0.007). The addition of “live bacteria” in the diet increased the pupal recovery rate over both control and autoclaved bacteria (Wald’s test t = 8.98, df = 1, P = 0.003, and t = 3.99, df = 1, P = 0.046 respectively). Interestingly, although the addition of “autoclaved bacteria” seems to lead also to an increased pupae recovery compared to “without bacteria” treatment, this difference was not significant (Wald’s test t = 2.499, df = 1, P = 0.114) (Fig 2A).

Bottom Line: The Mediterranean fruit fly (medfly), Ceratitis capitata, is a pest of worldwide substantial economic importance, as well as a Tephritidae model for sterile insect technique (SIT) applications.Recent studies have suggested that the manipulation of gut symbionts can have a significant positive effect on the overall fitness of insect strains.Probiotic application of Enterobacter sp. resulted in improvement of both pupal and adult productivity, as well as reduced rearing duration, particularly for males, without affecting pupal weight, sex ratio, male mating competitiveness, flight ability and longevity under starvation.

View Article: PubMed Central - PubMed

Affiliation: Insect Pest Control Laboratory, Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, Seibersdorf, Vienna, Austria; Department of Environmental and Natural Resources Management, University of Patras, Agrinio, Greece.

ABSTRACT
The Mediterranean fruit fly (medfly), Ceratitis capitata, is a pest of worldwide substantial economic importance, as well as a Tephritidae model for sterile insect technique (SIT) applications. The latter is partially due to the development and utilization of genetic sexing strains (GSS) for this species, such as the Vienna 8 strain, which is currently used in mass rearing facilities worldwide. Improving the performance of such a strain both in mass rearing facilities and in the field could significantly enhance the efficacy of SIT and reduce operational costs. Recent studies have suggested that the manipulation of gut symbionts can have a significant positive effect on the overall fitness of insect strains. We used culture-based approaches to isolate and characterize gut-associated bacterial species of the Vienna 8 strain under mass rearing conditions. We also exploited one of the isolated bacterial species, Enterobacter sp., as dietary supplement (probiotic) to the larval diet, and we assessed its effects on fitness parameters under the standard operating procedures used in SIT operational programs. Probiotic application of Enterobacter sp. resulted in improvement of both pupal and adult productivity, as well as reduced rearing duration, particularly for males, without affecting pupal weight, sex ratio, male mating competitiveness, flight ability and longevity under starvation.

No MeSH data available.


Related in: MedlinePlus