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Anticancer and apoptotic effects on cell proliferation of diosgenin isolated from Costus speciosus (Koen.) Sm.

Selim S, Al Jaouni S - BMC Complement Altern Med (2015)

Bottom Line: Diosgenin, a naturally occurring steroid saponin found abundantly in C. speciosus, is a well-known precursor of various synthetic steroidal drugs that are extensively used in the pharmaceutical industry.The results indicated that the treatment of HepG2 cells with the sample resulted in a cytotoxic effect as concluded from the IC50 value 32.62 μg/ml, while the treatment of HepG2 cells with paclitaxel, a known anti-cancer drug, resulted in an IC50 value of 0.48 μg/ml.The treatment of MCF-7 cells with the tested sample resulted in high inhibition in the cell viability, and resulted in an IC50 value of 11.03 μg/ml, while the treatment of MCF-7 cells with paclitaxel resulted in an IC50 value of 0.61 μg/ml.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Aljouf University, Sakaka, P.O. 2014, Saudi Arabia. sadomm2003@yahoo.com.

ABSTRACT

Background: Diosgenin, a naturally occurring steroid saponin found abundantly in C. speciosus, is a well-known precursor of various synthetic steroidal drugs that are extensively used in the pharmaceutical industry.

Methods: The present study was conducted to evaluate the in vitro anticancer and apoptotic effects on cell proliferation of diosgenin isolated from C. speciosus (Koen.) Sm.

Results: The results indicated that the treatment of HepG2 cells with the sample resulted in a cytotoxic effect as concluded from the IC50 value 32.62 μg/ml, while the treatment of HepG2 cells with paclitaxel, a known anti-cancer drug, resulted in an IC50 value of 0.48 μg/ml. The treatment of MCF-7 cells with the tested sample resulted in high inhibition in the cell viability, and resulted in an IC50 value of 11.03 μg/ml, while the treatment of MCF-7 cells with paclitaxel resulted in an IC50 value of 0.61 μg/ml. The levels of DR4 and caspase-3 were significantly increased (P<0.01) in MCF-7 cells treated with the tested sample compared to untreated cells and possessed a similar activity of paclitaxel in DR4 induction but lower induction in caspase-3. On the other hand the treatment of macrophages or lymphocytes with diosgenin (250 μg/ml) resulted in an induction in the cell proliferation up to 3.2-fold and 2.1-fold of control, respectively.

Conclusions: The results presented here may suggest that diosgenin isolated from C. speciosus possess anticancer and apoptotic effects on cell proliferation, and therefore, can be used as pharmaceuticals drugs.

No MeSH data available.


Related in: MedlinePlus

The proliferation (% of control) of different concentrations (μg/ml) of the tested sample in lymphoblastic leukemia 1301 cells (grey line) and Raw murine macrophage RAW 264.7 (black line) after 72 h of incubation as assayed by MTT. (n = 4)
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Fig7: The proliferation (% of control) of different concentrations (μg/ml) of the tested sample in lymphoblastic leukemia 1301 cells (grey line) and Raw murine macrophage RAW 264.7 (black line) after 72 h of incubation as assayed by MTT. (n = 4)

Mentions: Investigation of the effect of the tested sample on the proliferation of the immune cells was carried out using two different immune cell lines and assayed by a MTT test. The results indicated that the treatment of macrophages and lymphocytes with different sample concentrations resulted in no change in the proliferation index compared to the control untreated cells, as shown in Fig. 7. On the other hand the treatment of macrophages or lymphocytes with diosgenin (250 μg/ml) resulted in an induction in the cell proliferation up to 3.2-fold and 2.1-fold of control, respectively.Fig. 7


Anticancer and apoptotic effects on cell proliferation of diosgenin isolated from Costus speciosus (Koen.) Sm.

Selim S, Al Jaouni S - BMC Complement Altern Med (2015)

The proliferation (% of control) of different concentrations (μg/ml) of the tested sample in lymphoblastic leukemia 1301 cells (grey line) and Raw murine macrophage RAW 264.7 (black line) after 72 h of incubation as assayed by MTT. (n = 4)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4556405&req=5

Fig7: The proliferation (% of control) of different concentrations (μg/ml) of the tested sample in lymphoblastic leukemia 1301 cells (grey line) and Raw murine macrophage RAW 264.7 (black line) after 72 h of incubation as assayed by MTT. (n = 4)
Mentions: Investigation of the effect of the tested sample on the proliferation of the immune cells was carried out using two different immune cell lines and assayed by a MTT test. The results indicated that the treatment of macrophages and lymphocytes with different sample concentrations resulted in no change in the proliferation index compared to the control untreated cells, as shown in Fig. 7. On the other hand the treatment of macrophages or lymphocytes with diosgenin (250 μg/ml) resulted in an induction in the cell proliferation up to 3.2-fold and 2.1-fold of control, respectively.Fig. 7

Bottom Line: Diosgenin, a naturally occurring steroid saponin found abundantly in C. speciosus, is a well-known precursor of various synthetic steroidal drugs that are extensively used in the pharmaceutical industry.The results indicated that the treatment of HepG2 cells with the sample resulted in a cytotoxic effect as concluded from the IC50 value 32.62 μg/ml, while the treatment of HepG2 cells with paclitaxel, a known anti-cancer drug, resulted in an IC50 value of 0.48 μg/ml.The treatment of MCF-7 cells with the tested sample resulted in high inhibition in the cell viability, and resulted in an IC50 value of 11.03 μg/ml, while the treatment of MCF-7 cells with paclitaxel resulted in an IC50 value of 0.61 μg/ml.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Aljouf University, Sakaka, P.O. 2014, Saudi Arabia. sadomm2003@yahoo.com.

ABSTRACT

Background: Diosgenin, a naturally occurring steroid saponin found abundantly in C. speciosus, is a well-known precursor of various synthetic steroidal drugs that are extensively used in the pharmaceutical industry.

Methods: The present study was conducted to evaluate the in vitro anticancer and apoptotic effects on cell proliferation of diosgenin isolated from C. speciosus (Koen.) Sm.

Results: The results indicated that the treatment of HepG2 cells with the sample resulted in a cytotoxic effect as concluded from the IC50 value 32.62 μg/ml, while the treatment of HepG2 cells with paclitaxel, a known anti-cancer drug, resulted in an IC50 value of 0.48 μg/ml. The treatment of MCF-7 cells with the tested sample resulted in high inhibition in the cell viability, and resulted in an IC50 value of 11.03 μg/ml, while the treatment of MCF-7 cells with paclitaxel resulted in an IC50 value of 0.61 μg/ml. The levels of DR4 and caspase-3 were significantly increased (P<0.01) in MCF-7 cells treated with the tested sample compared to untreated cells and possessed a similar activity of paclitaxel in DR4 induction but lower induction in caspase-3. On the other hand the treatment of macrophages or lymphocytes with diosgenin (250 μg/ml) resulted in an induction in the cell proliferation up to 3.2-fold and 2.1-fold of control, respectively.

Conclusions: The results presented here may suggest that diosgenin isolated from C. speciosus possess anticancer and apoptotic effects on cell proliferation, and therefore, can be used as pharmaceuticals drugs.

No MeSH data available.


Related in: MedlinePlus