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Anticancer and apoptotic effects on cell proliferation of diosgenin isolated from Costus speciosus (Koen.) Sm.

Selim S, Al Jaouni S - BMC Complement Altern Med (2015)

Bottom Line: Diosgenin, a naturally occurring steroid saponin found abundantly in C. speciosus, is a well-known precursor of various synthetic steroidal drugs that are extensively used in the pharmaceutical industry.The results indicated that the treatment of HepG2 cells with the sample resulted in a cytotoxic effect as concluded from the IC50 value 32.62 μg/ml, while the treatment of HepG2 cells with paclitaxel, a known anti-cancer drug, resulted in an IC50 value of 0.48 μg/ml.The treatment of MCF-7 cells with the tested sample resulted in high inhibition in the cell viability, and resulted in an IC50 value of 11.03 μg/ml, while the treatment of MCF-7 cells with paclitaxel resulted in an IC50 value of 0.61 μg/ml.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Aljouf University, Sakaka, P.O. 2014, Saudi Arabia. sadomm2003@yahoo.com.

ABSTRACT

Background: Diosgenin, a naturally occurring steroid saponin found abundantly in C. speciosus, is a well-known precursor of various synthetic steroidal drugs that are extensively used in the pharmaceutical industry.

Methods: The present study was conducted to evaluate the in vitro anticancer and apoptotic effects on cell proliferation of diosgenin isolated from C. speciosus (Koen.) Sm.

Results: The results indicated that the treatment of HepG2 cells with the sample resulted in a cytotoxic effect as concluded from the IC50 value 32.62 μg/ml, while the treatment of HepG2 cells with paclitaxel, a known anti-cancer drug, resulted in an IC50 value of 0.48 μg/ml. The treatment of MCF-7 cells with the tested sample resulted in high inhibition in the cell viability, and resulted in an IC50 value of 11.03 μg/ml, while the treatment of MCF-7 cells with paclitaxel resulted in an IC50 value of 0.61 μg/ml. The levels of DR4 and caspase-3 were significantly increased (P<0.01) in MCF-7 cells treated with the tested sample compared to untreated cells and possessed a similar activity of paclitaxel in DR4 induction but lower induction in caspase-3. On the other hand the treatment of macrophages or lymphocytes with diosgenin (250 μg/ml) resulted in an induction in the cell proliferation up to 3.2-fold and 2.1-fold of control, respectively.

Conclusions: The results presented here may suggest that diosgenin isolated from C. speciosus possess anticancer and apoptotic effects on cell proliferation, and therefore, can be used as pharmaceuticals drugs.

No MeSH data available.


Related in: MedlinePlus

The effect of treatment with the tested sample (black bars, 50 % of IC50) on apoptotic cell death pathway in MCF-7 cells compared with paclitaxel activity (gray bars). DR4 and caspase-3 levels in treated cells were compared with control cells. The data represented as number of folds in relation to control absorbance readings, which were 7.3, and 6.5 milliabsorbance, respectively
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Fig6: The effect of treatment with the tested sample (black bars, 50 % of IC50) on apoptotic cell death pathway in MCF-7 cells compared with paclitaxel activity (gray bars). DR4 and caspase-3 levels in treated cells were compared with control cells. The data represented as number of folds in relation to control absorbance readings, which were 7.3, and 6.5 milliabsorbance, respectively

Mentions: Apoptosis can be started through two distinct pathways, the death receptor (extrinsic pathway) mitochondrial mediated (intrinsic pathway). Both lead to activation of the caspase cascades "executioner caspases" such as caspase-3, −6 and −7. The active executioner caspases bind cellular substrates, leading to the characteristic biochemical and morphological changes observed in dying cells. This is followed by chromatin condensation, nuclear shrinkage and DNA fragmentation. Binding of cytoskeletal proteins leads to cell fragmentation and formation of apoptotic bodies [22, 23]. As shown in Fig. 6, the levels of DR4 and caspase-3 were significantly increased (P < 0.01) in MCF-7 cells treated with the tested sample compared to untreated cells and possessed a similar activity of paclitaxel in DR4 induction but lower induction in caspase-3.Fig. 6


Anticancer and apoptotic effects on cell proliferation of diosgenin isolated from Costus speciosus (Koen.) Sm.

Selim S, Al Jaouni S - BMC Complement Altern Med (2015)

The effect of treatment with the tested sample (black bars, 50 % of IC50) on apoptotic cell death pathway in MCF-7 cells compared with paclitaxel activity (gray bars). DR4 and caspase-3 levels in treated cells were compared with control cells. The data represented as number of folds in relation to control absorbance readings, which were 7.3, and 6.5 milliabsorbance, respectively
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4556405&req=5

Fig6: The effect of treatment with the tested sample (black bars, 50 % of IC50) on apoptotic cell death pathway in MCF-7 cells compared with paclitaxel activity (gray bars). DR4 and caspase-3 levels in treated cells were compared with control cells. The data represented as number of folds in relation to control absorbance readings, which were 7.3, and 6.5 milliabsorbance, respectively
Mentions: Apoptosis can be started through two distinct pathways, the death receptor (extrinsic pathway) mitochondrial mediated (intrinsic pathway). Both lead to activation of the caspase cascades "executioner caspases" such as caspase-3, −6 and −7. The active executioner caspases bind cellular substrates, leading to the characteristic biochemical and morphological changes observed in dying cells. This is followed by chromatin condensation, nuclear shrinkage and DNA fragmentation. Binding of cytoskeletal proteins leads to cell fragmentation and formation of apoptotic bodies [22, 23]. As shown in Fig. 6, the levels of DR4 and caspase-3 were significantly increased (P < 0.01) in MCF-7 cells treated with the tested sample compared to untreated cells and possessed a similar activity of paclitaxel in DR4 induction but lower induction in caspase-3.Fig. 6

Bottom Line: Diosgenin, a naturally occurring steroid saponin found abundantly in C. speciosus, is a well-known precursor of various synthetic steroidal drugs that are extensively used in the pharmaceutical industry.The results indicated that the treatment of HepG2 cells with the sample resulted in a cytotoxic effect as concluded from the IC50 value 32.62 μg/ml, while the treatment of HepG2 cells with paclitaxel, a known anti-cancer drug, resulted in an IC50 value of 0.48 μg/ml.The treatment of MCF-7 cells with the tested sample resulted in high inhibition in the cell viability, and resulted in an IC50 value of 11.03 μg/ml, while the treatment of MCF-7 cells with paclitaxel resulted in an IC50 value of 0.61 μg/ml.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Aljouf University, Sakaka, P.O. 2014, Saudi Arabia. sadomm2003@yahoo.com.

ABSTRACT

Background: Diosgenin, a naturally occurring steroid saponin found abundantly in C. speciosus, is a well-known precursor of various synthetic steroidal drugs that are extensively used in the pharmaceutical industry.

Methods: The present study was conducted to evaluate the in vitro anticancer and apoptotic effects on cell proliferation of diosgenin isolated from C. speciosus (Koen.) Sm.

Results: The results indicated that the treatment of HepG2 cells with the sample resulted in a cytotoxic effect as concluded from the IC50 value 32.62 μg/ml, while the treatment of HepG2 cells with paclitaxel, a known anti-cancer drug, resulted in an IC50 value of 0.48 μg/ml. The treatment of MCF-7 cells with the tested sample resulted in high inhibition in the cell viability, and resulted in an IC50 value of 11.03 μg/ml, while the treatment of MCF-7 cells with paclitaxel resulted in an IC50 value of 0.61 μg/ml. The levels of DR4 and caspase-3 were significantly increased (P<0.01) in MCF-7 cells treated with the tested sample compared to untreated cells and possessed a similar activity of paclitaxel in DR4 induction but lower induction in caspase-3. On the other hand the treatment of macrophages or lymphocytes with diosgenin (250 μg/ml) resulted in an induction in the cell proliferation up to 3.2-fold and 2.1-fold of control, respectively.

Conclusions: The results presented here may suggest that diosgenin isolated from C. speciosus possess anticancer and apoptotic effects on cell proliferation, and therefore, can be used as pharmaceuticals drugs.

No MeSH data available.


Related in: MedlinePlus