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Anticancer and apoptotic effects on cell proliferation of diosgenin isolated from Costus speciosus (Koen.) Sm.

Selim S, Al Jaouni S - BMC Complement Altern Med (2015)

Bottom Line: Diosgenin, a naturally occurring steroid saponin found abundantly in C. speciosus, is a well-known precursor of various synthetic steroidal drugs that are extensively used in the pharmaceutical industry.The results indicated that the treatment of HepG2 cells with the sample resulted in a cytotoxic effect as concluded from the IC50 value 32.62 μg/ml, while the treatment of HepG2 cells with paclitaxel, a known anti-cancer drug, resulted in an IC50 value of 0.48 μg/ml.The treatment of MCF-7 cells with the tested sample resulted in high inhibition in the cell viability, and resulted in an IC50 value of 11.03 μg/ml, while the treatment of MCF-7 cells with paclitaxel resulted in an IC50 value of 0.61 μg/ml.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Aljouf University, Sakaka, P.O. 2014, Saudi Arabia. sadomm2003@yahoo.com.

ABSTRACT

Background: Diosgenin, a naturally occurring steroid saponin found abundantly in C. speciosus, is a well-known precursor of various synthetic steroidal drugs that are extensively used in the pharmaceutical industry.

Methods: The present study was conducted to evaluate the in vitro anticancer and apoptotic effects on cell proliferation of diosgenin isolated from C. speciosus (Koen.) Sm.

Results: The results indicated that the treatment of HepG2 cells with the sample resulted in a cytotoxic effect as concluded from the IC50 value 32.62 μg/ml, while the treatment of HepG2 cells with paclitaxel, a known anti-cancer drug, resulted in an IC50 value of 0.48 μg/ml. The treatment of MCF-7 cells with the tested sample resulted in high inhibition in the cell viability, and resulted in an IC50 value of 11.03 μg/ml, while the treatment of MCF-7 cells with paclitaxel resulted in an IC50 value of 0.61 μg/ml. The levels of DR4 and caspase-3 were significantly increased (P<0.01) in MCF-7 cells treated with the tested sample compared to untreated cells and possessed a similar activity of paclitaxel in DR4 induction but lower induction in caspase-3. On the other hand the treatment of macrophages or lymphocytes with diosgenin (250 μg/ml) resulted in an induction in the cell proliferation up to 3.2-fold and 2.1-fold of control, respectively.

Conclusions: The results presented here may suggest that diosgenin isolated from C. speciosus possess anticancer and apoptotic effects on cell proliferation, and therefore, can be used as pharmaceuticals drugs.

No MeSH data available.


Related in: MedlinePlus

The cell population percentage of vital (white segments), apoptotic (black segment) and necrotic (gray segments) cells were counted after treatment with the tested sample and paclitaxel. Results represent the mean of three independent experiments
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Fig5: The cell population percentage of vital (white segments), apoptotic (black segment) and necrotic (gray segments) cells were counted after treatment with the tested sample and paclitaxel. Results represent the mean of three independent experiments

Mentions: Acridine orange (AO) stained both live and dead cells, while ethidium bromide (EB) stained only cells with lost membrane integrity. Living cells will appear green, while early apoptotic cells are containing bright green to yellow dots in the nuclei due to chromatin condensation and nuclear fragmentation. Late apoptotic cells also have EB (orange), but unlike necrotic cells, the late apoptotic cells exhibited condensed and fragmented nuclei. Necrotic cells are in deep orange to red, but have a nuclear morphology resembling that of living cells, with no condensed chromatin. The percentages of vital, apoptotic and necrotic cells were determined and compared with untreated cells, as demonstrated in Fig. 4. As shown in Fig. 5, MCF-7 cells treated with the tested sample and paclitaxel, using half IC50 concentration of each, showed a significant (P < 0.05) increase in the total percentage of cell death into >50 %, where there was a significant increase in both of apoptotic and necrotic cell populations, however the majority population in dead cells in the tested sample and paclitaxel was apoptotic population.Fig. 4


Anticancer and apoptotic effects on cell proliferation of diosgenin isolated from Costus speciosus (Koen.) Sm.

Selim S, Al Jaouni S - BMC Complement Altern Med (2015)

The cell population percentage of vital (white segments), apoptotic (black segment) and necrotic (gray segments) cells were counted after treatment with the tested sample and paclitaxel. Results represent the mean of three independent experiments
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4556405&req=5

Fig5: The cell population percentage of vital (white segments), apoptotic (black segment) and necrotic (gray segments) cells were counted after treatment with the tested sample and paclitaxel. Results represent the mean of three independent experiments
Mentions: Acridine orange (AO) stained both live and dead cells, while ethidium bromide (EB) stained only cells with lost membrane integrity. Living cells will appear green, while early apoptotic cells are containing bright green to yellow dots in the nuclei due to chromatin condensation and nuclear fragmentation. Late apoptotic cells also have EB (orange), but unlike necrotic cells, the late apoptotic cells exhibited condensed and fragmented nuclei. Necrotic cells are in deep orange to red, but have a nuclear morphology resembling that of living cells, with no condensed chromatin. The percentages of vital, apoptotic and necrotic cells were determined and compared with untreated cells, as demonstrated in Fig. 4. As shown in Fig. 5, MCF-7 cells treated with the tested sample and paclitaxel, using half IC50 concentration of each, showed a significant (P < 0.05) increase in the total percentage of cell death into >50 %, where there was a significant increase in both of apoptotic and necrotic cell populations, however the majority population in dead cells in the tested sample and paclitaxel was apoptotic population.Fig. 4

Bottom Line: Diosgenin, a naturally occurring steroid saponin found abundantly in C. speciosus, is a well-known precursor of various synthetic steroidal drugs that are extensively used in the pharmaceutical industry.The results indicated that the treatment of HepG2 cells with the sample resulted in a cytotoxic effect as concluded from the IC50 value 32.62 μg/ml, while the treatment of HepG2 cells with paclitaxel, a known anti-cancer drug, resulted in an IC50 value of 0.48 μg/ml.The treatment of MCF-7 cells with the tested sample resulted in high inhibition in the cell viability, and resulted in an IC50 value of 11.03 μg/ml, while the treatment of MCF-7 cells with paclitaxel resulted in an IC50 value of 0.61 μg/ml.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Aljouf University, Sakaka, P.O. 2014, Saudi Arabia. sadomm2003@yahoo.com.

ABSTRACT

Background: Diosgenin, a naturally occurring steroid saponin found abundantly in C. speciosus, is a well-known precursor of various synthetic steroidal drugs that are extensively used in the pharmaceutical industry.

Methods: The present study was conducted to evaluate the in vitro anticancer and apoptotic effects on cell proliferation of diosgenin isolated from C. speciosus (Koen.) Sm.

Results: The results indicated that the treatment of HepG2 cells with the sample resulted in a cytotoxic effect as concluded from the IC50 value 32.62 μg/ml, while the treatment of HepG2 cells with paclitaxel, a known anti-cancer drug, resulted in an IC50 value of 0.48 μg/ml. The treatment of MCF-7 cells with the tested sample resulted in high inhibition in the cell viability, and resulted in an IC50 value of 11.03 μg/ml, while the treatment of MCF-7 cells with paclitaxel resulted in an IC50 value of 0.61 μg/ml. The levels of DR4 and caspase-3 were significantly increased (P<0.01) in MCF-7 cells treated with the tested sample compared to untreated cells and possessed a similar activity of paclitaxel in DR4 induction but lower induction in caspase-3. On the other hand the treatment of macrophages or lymphocytes with diosgenin (250 μg/ml) resulted in an induction in the cell proliferation up to 3.2-fold and 2.1-fold of control, respectively.

Conclusions: The results presented here may suggest that diosgenin isolated from C. speciosus possess anticancer and apoptotic effects on cell proliferation, and therefore, can be used as pharmaceuticals drugs.

No MeSH data available.


Related in: MedlinePlus