Limits...
Quantitative proteomic analysis of mitochondria from human ovarian cancer cells and their paclitaxel-resistant sublines.

Chen M, Huang H, He H, Ying W, Liu X, Dai Z, Yin J, Mao N, Qian X, Pan L - Cancer Sci. (2015)

Bottom Line: Identification of the relevant proteins in mitochondria will help in clarifying the possible mechanisms and in selecting effective chemotherapy for patients with paclitaxel resistance.Among them, mimitin and 14-3-3 ζ/δ were selected for further research.Multivariate analyses demonstrated that lower expression levels of the mimitin and 14-3-3 ζ/δ proteins were positively associated with shorter progression-free survival (PFS) and overall survival (OS) in patients with primary ovarian cancer (mimitin: PFS: P = 0.041, OS: P = 0.003; 14-3-3 ζ/δ: PFS: P = 0.031, OS: P = 0.011).

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Show MeSH

Related in: MedlinePlus

Western blotting was used to validate the expression levels of mimitin and 14-3-3 ζ/δ in two paclitaxel-resistant cell lines, SKOV3-TR (TSmt) and A2780-TR (TAmt), and their parental sensitive cell lines, SKOV3 (Smt) and A2780 (Amt). The proteins were located at 20 and 28 kDa. COX4 was the loading control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4556398&req=5

fig03: Western blotting was used to validate the expression levels of mimitin and 14-3-3 ζ/δ in two paclitaxel-resistant cell lines, SKOV3-TR (TSmt) and A2780-TR (TAmt), and their parental sensitive cell lines, SKOV3 (Smt) and A2780 (Amt). The proteins were located at 20 and 28 kDa. COX4 was the loading control.

Mentions: To validate the expression levels of mimitin and 14-3-3 ζ/δ identified by LC-FTICR MS, the protein levels in mitochondrion-enriched fractions were measured in each cell line using western blotting. As shown in Figure3, the protein levels of mimitin and 14-3-3 ζ/δ were higher in the SKOV3 and A2780 cell lines than in their paclitaxel-resistant counterparts. The results were consistent with those of the LC-FTICR MS.


Quantitative proteomic analysis of mitochondria from human ovarian cancer cells and their paclitaxel-resistant sublines.

Chen M, Huang H, He H, Ying W, Liu X, Dai Z, Yin J, Mao N, Qian X, Pan L - Cancer Sci. (2015)

Western blotting was used to validate the expression levels of mimitin and 14-3-3 ζ/δ in two paclitaxel-resistant cell lines, SKOV3-TR (TSmt) and A2780-TR (TAmt), and their parental sensitive cell lines, SKOV3 (Smt) and A2780 (Amt). The proteins were located at 20 and 28 kDa. COX4 was the loading control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556398&req=5

fig03: Western blotting was used to validate the expression levels of mimitin and 14-3-3 ζ/δ in two paclitaxel-resistant cell lines, SKOV3-TR (TSmt) and A2780-TR (TAmt), and their parental sensitive cell lines, SKOV3 (Smt) and A2780 (Amt). The proteins were located at 20 and 28 kDa. COX4 was the loading control.
Mentions: To validate the expression levels of mimitin and 14-3-3 ζ/δ identified by LC-FTICR MS, the protein levels in mitochondrion-enriched fractions were measured in each cell line using western blotting. As shown in Figure3, the protein levels of mimitin and 14-3-3 ζ/δ were higher in the SKOV3 and A2780 cell lines than in their paclitaxel-resistant counterparts. The results were consistent with those of the LC-FTICR MS.

Bottom Line: Identification of the relevant proteins in mitochondria will help in clarifying the possible mechanisms and in selecting effective chemotherapy for patients with paclitaxel resistance.Among them, mimitin and 14-3-3 ζ/δ were selected for further research.Multivariate analyses demonstrated that lower expression levels of the mimitin and 14-3-3 ζ/δ proteins were positively associated with shorter progression-free survival (PFS) and overall survival (OS) in patients with primary ovarian cancer (mimitin: PFS: P = 0.041, OS: P = 0.003; 14-3-3 ζ/δ: PFS: P = 0.031, OS: P = 0.011).

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Show MeSH
Related in: MedlinePlus