Quantitative proteomic analysis of mitochondria from human ovarian cancer cells and their paclitaxel-resistant sublines.
Bottom Line: Identification of the relevant proteins in mitochondria will help in clarifying the possible mechanisms and in selecting effective chemotherapy for patients with paclitaxel resistance.Among them, mimitin and 14-3-3 ζ/δ were selected for further research.Multivariate analyses demonstrated that lower expression levels of the mimitin and 14-3-3 ζ/δ proteins were positively associated with shorter progression-free survival (PFS) and overall survival (OS) in patients with primary ovarian cancer (mimitin: PFS: P = 0.041, OS: P = 0.003; 14-3-3 ζ/δ: PFS: P = 0.031, OS: P = 0.011).
Affiliation: Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.Show MeSH
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Mentions: To validate the expression levels of mimitin and 14-3-3 ζ/δ identified by LC-FTICR MS, the protein levels in mitochondrion-enriched fractions were measured in each cell line using western blotting. As shown in Figure3, the protein levels of mimitin and 14-3-3 ζ/δ were higher in the SKOV3 and A2780 cell lines than in their paclitaxel-resistant counterparts. The results were consistent with those of the LC-FTICR MS.
Affiliation: Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.