53BP1 suppresses epithelial-mesenchymal transition by downregulating ZEB1 through microRNA-200b/429 in breast cancer.
Bottom Line: Consistently, in MCF-7 breast cancer cells, low 53BP1 expression reduced E-cadherin expression, resulting in increased migration and invasion.These effects were reversed by miR-200b and miR-429 inhibition or overexpression.It was also found that 53BP1 was associated with lymph node metastasis.
Affiliation: Department of Breast Surgery, Qilu Hospital, Shandong University, Jinan, China.Show MeSH
Related in: MedlinePlus
Mentions: Our results were confirmed in vivo in a tumor xenograft mice model conducted previously. Immunohistochemistry analysis of MDA-MB-231-53BP1 tumors showed that ZEB1 and vimentin expression decreased, whereas E-cadherin expression increased. Consistently, ZEB1 and vimentin increased and E-cadherin expression decreased in MCF-7-sh53BP1 tumor sections (Fig.5). Taken together, these data indicated that 53BP1 is a positive regulator of E-cadherin through downregulating the expression of the E-cadherin repressor ZEB1.
Affiliation: Department of Breast Surgery, Qilu Hospital, Shandong University, Jinan, China.