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53BP1 suppresses epithelial-mesenchymal transition by downregulating ZEB1 through microRNA-200b/429 in breast cancer.

Kong X, Ding X, Li X, Gao S, Yang Q - Cancer Sci. (2015)

Bottom Line: Consistently, in MCF-7 breast cancer cells, low 53BP1 expression reduced E-cadherin expression, resulting in increased migration and invasion.These effects were reversed by miR-200b and miR-429 inhibition or overexpression.It was also found that 53BP1 was associated with lymph node metastasis.

View Article: PubMed Central - PubMed

Affiliation: Department of Breast Surgery, Qilu Hospital, Shandong University, Jinan, China.

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Related in: MedlinePlus

Negative effects of 53BP1 on migration and invasion were mediated by miR-200b and miR-429 in breast cancer cells. (a) MDA-MB-231-53BP1 overexpressed cells were transfected with miR-Control (Con) and miR-200b/429 inhibitors (Inh). Migration and invasion assays were carried out. (b) MCF-7-sh53BP1 cells were transfected with miR-Control and miR-200b/429 mimics. Migration and invasion assays were carried out. All experiments were performed at least three times. Error bars, ±SEM. *P < 0.05 versus miR-Control, #P < 0.05 versus miR-Control (Student’s t-test).
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fig04: Negative effects of 53BP1 on migration and invasion were mediated by miR-200b and miR-429 in breast cancer cells. (a) MDA-MB-231-53BP1 overexpressed cells were transfected with miR-Control (Con) and miR-200b/429 inhibitors (Inh). Migration and invasion assays were carried out. (b) MCF-7-sh53BP1 cells were transfected with miR-Control and miR-200b/429 mimics. Migration and invasion assays were carried out. All experiments were performed at least three times. Error bars, ±SEM. *P < 0.05 versus miR-Control, #P < 0.05 versus miR-Control (Student’s t-test).

Mentions: Furthermore, treatments with miR-200b/429 inhibitors and mimics reversed the effects of 53BP1 on migration and invasion in MDA-MB-231-53BP1 cells and MCF-7-sh53BP1 cells (Fig.4), indicating that miR-200b/429 mediated 53BP1’s negative regulation of migration and invasion.


53BP1 suppresses epithelial-mesenchymal transition by downregulating ZEB1 through microRNA-200b/429 in breast cancer.

Kong X, Ding X, Li X, Gao S, Yang Q - Cancer Sci. (2015)

Negative effects of 53BP1 on migration and invasion were mediated by miR-200b and miR-429 in breast cancer cells. (a) MDA-MB-231-53BP1 overexpressed cells were transfected with miR-Control (Con) and miR-200b/429 inhibitors (Inh). Migration and invasion assays were carried out. (b) MCF-7-sh53BP1 cells were transfected with miR-Control and miR-200b/429 mimics. Migration and invasion assays were carried out. All experiments were performed at least three times. Error bars, ±SEM. *P < 0.05 versus miR-Control, #P < 0.05 versus miR-Control (Student’s t-test).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556386&req=5

fig04: Negative effects of 53BP1 on migration and invasion were mediated by miR-200b and miR-429 in breast cancer cells. (a) MDA-MB-231-53BP1 overexpressed cells were transfected with miR-Control (Con) and miR-200b/429 inhibitors (Inh). Migration and invasion assays were carried out. (b) MCF-7-sh53BP1 cells were transfected with miR-Control and miR-200b/429 mimics. Migration and invasion assays were carried out. All experiments were performed at least three times. Error bars, ±SEM. *P < 0.05 versus miR-Control, #P < 0.05 versus miR-Control (Student’s t-test).
Mentions: Furthermore, treatments with miR-200b/429 inhibitors and mimics reversed the effects of 53BP1 on migration and invasion in MDA-MB-231-53BP1 cells and MCF-7-sh53BP1 cells (Fig.4), indicating that miR-200b/429 mediated 53BP1’s negative regulation of migration and invasion.

Bottom Line: Consistently, in MCF-7 breast cancer cells, low 53BP1 expression reduced E-cadherin expression, resulting in increased migration and invasion.These effects were reversed by miR-200b and miR-429 inhibition or overexpression.It was also found that 53BP1 was associated with lymph node metastasis.

View Article: PubMed Central - PubMed

Affiliation: Department of Breast Surgery, Qilu Hospital, Shandong University, Jinan, China.

Show MeSH
Related in: MedlinePlus