Differential reactivation of fetal/neonatal genes in mouse liver tumors induced in cirrhotic and non-cirrhotic conditions.
Bottom Line: We identified tumor-associated genes that were expressed differentially in the cirrhotic CCl4 model (H19, Igf2, Cbr3, and Krt20) and the non-cirrhotic DEN model (Tff3, Akr1c18, Gpc3, Afp, and Abcd2) as well as genes that were expressed comparably in both models (Ly6d, Slpi, Spink3, Scd2, and Cpe).The levels and patterns of mRNA expression of these genes were validated by quantitative RT-PCR analyses.There was a close relationship between the expression levels of Igf2 and H19 mRNA, which were activated in the cirrhotic models.
Affiliation: Division of Tumor Pathology, Department of Pathology, Asahikawa Medical University, Asahikawa, Japan.Show MeSH
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Mentions: The mRNA expression data of the 15 tumor-associated genes in the four different liver tumor models (CCl4-induced, DEN-induced, TAA-induced, spontaneous) were subjected to unsupervised 2-D hierarchical cluster analysis (Fig.6). Interestingly, the mRNA expression profiles of the 15 genes almost clearly segregated control liver tissues, cirrhotic tissues, and tumors that had developed in the cirrhotic and non-cirrhotic backgrounds. Furthermore, there were several clusters of between two and four transcripts, which characterized tumors that had developed in the cirrhotic background (H19, Igf2, and Igf2bp3), in the non-cirrhotic background (Tff3, Akr1c18, Abcd2, and Gpc3), and in either background (Scd2 and Slpi; Cpe and Ly6d).
Affiliation: Division of Tumor Pathology, Department of Pathology, Asahikawa Medical University, Asahikawa, Japan.