Differential reactivation of fetal/neonatal genes in mouse liver tumors induced in cirrhotic and non-cirrhotic conditions.
Bottom Line: We identified tumor-associated genes that were expressed differentially in the cirrhotic CCl4 model (H19, Igf2, Cbr3, and Krt20) and the non-cirrhotic DEN model (Tff3, Akr1c18, Gpc3, Afp, and Abcd2) as well as genes that were expressed comparably in both models (Ly6d, Slpi, Spink3, Scd2, and Cpe).The levels and patterns of mRNA expression of these genes were validated by quantitative RT-PCR analyses.There was a close relationship between the expression levels of Igf2 and H19 mRNA, which were activated in the cirrhotic models.
Affiliation: Division of Tumor Pathology, Department of Pathology, Asahikawa Medical University, Asahikawa, Japan.Show MeSH
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Mentions: Because the identified tumor-associated genes included well-known oncofetal genes, such as Igf2, H19, Gpc3, and Afp, we examined their mRNA expression during fetal and neonatal periods. Our results clearly showed that all of these genes, with the exceptions of Cbr3 and Cpe, were highly expressed in either the fetal or neonatal periods (Fig.4a), indicating that the differential activation of fetal/neonatal gene expression occurs in CCl4- and DEN-induced liver tumors. As expected, the protein expression of IGF2 and TFF3 and the mRNA expression of H19 were detected in hepatoblasts/hepatocytes during the fetal or neonatal period (Fig.4b).
Affiliation: Division of Tumor Pathology, Department of Pathology, Asahikawa Medical University, Asahikawa, Japan.