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Differential reactivation of fetal/neonatal genes in mouse liver tumors induced in cirrhotic and non-cirrhotic conditions.

Chen X, Yamamoto M, Fujii K, Nagahama Y, Ooshio T, Xin B, Okada Y, Furukawa H, Nishikawa Y - Cancer Sci. (2015)

Bottom Line: We identified tumor-associated genes that were expressed differentially in the cirrhotic CCl4 model (H19, Igf2, Cbr3, and Krt20) and the non-cirrhotic DEN model (Tff3, Akr1c18, Gpc3, Afp, and Abcd2) as well as genes that were expressed comparably in both models (Ly6d, Slpi, Spink3, Scd2, and Cpe).The levels and patterns of mRNA expression of these genes were validated by quantitative RT-PCR analyses.There was a close relationship between the expression levels of Igf2 and H19 mRNA, which were activated in the cirrhotic models.

View Article: PubMed Central - PubMed

Affiliation: Division of Tumor Pathology, Department of Pathology, Asahikawa Medical University, Asahikawa, Japan.

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Relationship between mRNA expression of tumor-associated genes and tumor cell proliferation. Scatter plots of mRNA expression levels of selected tumor-associated genes and Ki-67 labeling index (%) in CCl4-induced tumors (n = 16) and diethylnitrosamine (DEN)-induced tumors (n = 15). Spearman correlation coefficients were used to test the association of mRNA expression and tumor cell proliferation.
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fig03: Relationship between mRNA expression of tumor-associated genes and tumor cell proliferation. Scatter plots of mRNA expression levels of selected tumor-associated genes and Ki-67 labeling index (%) in CCl4-induced tumors (n = 16) and diethylnitrosamine (DEN)-induced tumors (n = 15). Spearman correlation coefficients were used to test the association of mRNA expression and tumor cell proliferation.

Mentions: We next examined whether the mRNA expression of the tumor-associated genes correlated with tumor cell proliferation, as estimated by Ki-67 immunohistochemistry. In the CCl4 model, the expression levels of Cbr3 and Tff3 were correlated with the proliferative activity of the tumor cells (Fig.3). Although Igf2 encodes IGF2, which has been shown to play important roles in cell growth, Igf2 mRNA expression was not significantly correlated with tumor cell proliferation, similar to other genes (Figs3,S2). In the DEN model, the expression of none of the genes analyzed was related to the proliferative activity of the tumor cells (Figs3,S2).


Differential reactivation of fetal/neonatal genes in mouse liver tumors induced in cirrhotic and non-cirrhotic conditions.

Chen X, Yamamoto M, Fujii K, Nagahama Y, Ooshio T, Xin B, Okada Y, Furukawa H, Nishikawa Y - Cancer Sci. (2015)

Relationship between mRNA expression of tumor-associated genes and tumor cell proliferation. Scatter plots of mRNA expression levels of selected tumor-associated genes and Ki-67 labeling index (%) in CCl4-induced tumors (n = 16) and diethylnitrosamine (DEN)-induced tumors (n = 15). Spearman correlation coefficients were used to test the association of mRNA expression and tumor cell proliferation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556385&req=5

fig03: Relationship between mRNA expression of tumor-associated genes and tumor cell proliferation. Scatter plots of mRNA expression levels of selected tumor-associated genes and Ki-67 labeling index (%) in CCl4-induced tumors (n = 16) and diethylnitrosamine (DEN)-induced tumors (n = 15). Spearman correlation coefficients were used to test the association of mRNA expression and tumor cell proliferation.
Mentions: We next examined whether the mRNA expression of the tumor-associated genes correlated with tumor cell proliferation, as estimated by Ki-67 immunohistochemistry. In the CCl4 model, the expression levels of Cbr3 and Tff3 were correlated with the proliferative activity of the tumor cells (Fig.3). Although Igf2 encodes IGF2, which has been shown to play important roles in cell growth, Igf2 mRNA expression was not significantly correlated with tumor cell proliferation, similar to other genes (Figs3,S2). In the DEN model, the expression of none of the genes analyzed was related to the proliferative activity of the tumor cells (Figs3,S2).

Bottom Line: We identified tumor-associated genes that were expressed differentially in the cirrhotic CCl4 model (H19, Igf2, Cbr3, and Krt20) and the non-cirrhotic DEN model (Tff3, Akr1c18, Gpc3, Afp, and Abcd2) as well as genes that were expressed comparably in both models (Ly6d, Slpi, Spink3, Scd2, and Cpe).The levels and patterns of mRNA expression of these genes were validated by quantitative RT-PCR analyses.There was a close relationship between the expression levels of Igf2 and H19 mRNA, which were activated in the cirrhotic models.

View Article: PubMed Central - PubMed

Affiliation: Division of Tumor Pathology, Department of Pathology, Asahikawa Medical University, Asahikawa, Japan.

Show MeSH
Related in: MedlinePlus