Differential reactivation of fetal/neonatal genes in mouse liver tumors induced in cirrhotic and non-cirrhotic conditions.
Bottom Line: We identified tumor-associated genes that were expressed differentially in the cirrhotic CCl4 model (H19, Igf2, Cbr3, and Krt20) and the non-cirrhotic DEN model (Tff3, Akr1c18, Gpc3, Afp, and Abcd2) as well as genes that were expressed comparably in both models (Ly6d, Slpi, Spink3, Scd2, and Cpe).The levels and patterns of mRNA expression of these genes were validated by quantitative RT-PCR analyses.There was a close relationship between the expression levels of Igf2 and H19 mRNA, which were activated in the cirrhotic models.
Affiliation: Division of Tumor Pathology, Department of Pathology, Asahikawa Medical University, Asahikawa, Japan.Show MeSH
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Mentions: We next examined whether the mRNA expression of the tumor-associated genes correlated with tumor cell proliferation, as estimated by Ki-67 immunohistochemistry. In the CCl4 model, the expression levels of Cbr3 and Tff3 were correlated with the proliferative activity of the tumor cells (Fig.3). Although Igf2 encodes IGF2, which has been shown to play important roles in cell growth, Igf2 mRNA expression was not significantly correlated with tumor cell proliferation, similar to other genes (Figs3,S2). In the DEN model, the expression of none of the genes analyzed was related to the proliferative activity of the tumor cells (Figs3,S2).
Affiliation: Division of Tumor Pathology, Department of Pathology, Asahikawa Medical University, Asahikawa, Japan.