Basophil activation by mosquito extracts in patients with hypersensitivity to mosquito bites.
Bottom Line: We found evidence of EBV infection in NK cells through in situ hybridization that detected EBV-encoded small RNA-1, and flow cytometry showed HLA-DR expression on almost all NK cells.Basophil activation tests with the extracts of epidemic mosquitoes Culex pipiens pallens and Aedes albopictus showed positive responses to one or both extracts in all samples from patients with HMB, suggesting the presence of mosquito antigen-specific IgE and its binding to basophils.In particular, the extract of Aedes albopictus was able to activate basophils in all available patient samples.
Affiliation: Department of Pediatrics, School of Medicine, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.Show MeSH
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Mentions: As shown in Figure3(a), basophil activation occurred with exposure to the extracts of epidemic mosquitoes in all samples from patients with HMB, but no activation occurred in the absence of stimulation. Basophils from normal controls were not activated by mosquito antigens. When we examined in detail basophil activation on exposure to the respective antigens in samples from patients with HMB, we detected basophil activation on exposure to the extract of Ae. Albopictus, except in P4. Basophils from P2, P3 and P4 were activated by the extract of C. p. pallens. Both the extracts activated basophils in P2 and P3.
Affiliation: Department of Pediatrics, School of Medicine, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.