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Livestock-Associated Methicillin Resistant and Methicillin Susceptible Staphylococcus aureus Sequence Type (CC)1 in European Farmed Animals: High Genetic Relatedness of Isolates from Italian Cattle Herds and Humans.

Alba P, Feltrin F, Cordaro G, Porrero MC, Kraushaar B, Argudín MA, Nykäsenoja S, Monaco M, Stegger M, Aarestrup FM, Butaye P, Franco A, Battisti A - PLoS ONE (2015)

Bottom Line: They often showed genetic features typical of human adaptation or present in human-associated CC1: Immune evasion cluster (IEC) genes sak and scn, or sea; sat and aphA3-mediated aminoglycoside resistance.As such, these strains may represent a greater human hazard than the porcine strains.The zoonotic capacity of CC1 LA-MRSA from livestock must be taken seriously and measures should be implemented at farm-level to prevent spill-over.

View Article: PubMed Central - PubMed

Affiliation: Istituto Zooprofilattico Sperimentale del Lazio e della Toscana "M. Aleandri", Diagnostic Department, Rome, Italy.

ABSTRACT
Methicillin-resistant Staphylococcus aureus (MRSA) Sequence Type (ST)1, Clonal Complex(CC)1, SCCmec V is one of the major Livestock-Associated (LA-) lineages in pig farming industry in Italy and is associated with pigs in other European countries. Recently, it has been increasingly detected in Italian dairy cattle herds. The aim of this study was to analyse the differences between ST1 MRSA and methicillin-susceptible S. aureus (MSSA) from cattle and pig herds in Italy and Europe and human isolates. Sixty-tree animal isolates from different holdings and 20 human isolates were characterized by pulsed-field gel electrophoresis (PFGE), spa-typing, SCCmec typing, and by micro-array analysis for several virulence, antimicrobial resistance, and strain/host-specific marker genes. Three major PFGE clusters were detected. The bovine isolates shared a high (≥90% to 100%) similarity with human isolates and carried the same SCCmec type IVa. They often showed genetic features typical of human adaptation or present in human-associated CC1: Immune evasion cluster (IEC) genes sak and scn, or sea; sat and aphA3-mediated aminoglycoside resistance. Contrary, typical markers of porcine origin in Italy and Spain, like erm(A) mediated macrolide-lincosamide-streptograminB, and of vga(A)-mediated pleuromutilin resistance were always absent in human and bovine isolates. Most of ST(CC)1 MRSA from dairy cattle were multidrug-resistant and contained virulence and immunomodulatory genes associated with full capability of colonizing humans. As such, these strains may represent a greater human hazard than the porcine strains. The zoonotic capacity of CC1 LA-MRSA from livestock must be taken seriously and measures should be implemented at farm-level to prevent spill-over.

No MeSH data available.


Related in: MedlinePlus

Dendrogram of SmaI PFGE macrorestriction patterns, with selected virulence and antimicrobial resistance marker genes and elements of Sequence Type 1, Clonal Complex 1 MSSA and MRSA from animals and humans.Legend: † LukF-PV(P83)-LukM; ‡ vwb (COL+MW2); ☼Ciprofloxacin resistance; § PFGE profile. Notes: *SCCmecV&SCCfus; ** SCCmec IV (2B&5), subtype IVa.
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pone.0137143.g001: Dendrogram of SmaI PFGE macrorestriction patterns, with selected virulence and antimicrobial resistance marker genes and elements of Sequence Type 1, Clonal Complex 1 MSSA and MRSA from animals and humans.Legend: † LukF-PV(P83)-LukM; ‡ vwb (COL+MW2); ☼Ciprofloxacin resistance; § PFGE profile. Notes: *SCCmecV&SCCfus; ** SCCmec IV (2B&5), subtype IVa.

Mentions: A total of 83 ST(CC)1 S. aureus (71 MRSA and 12 MSSA) were studied (Fig 1). The majority (n = 65) were from Italy (including 30 MRSA from pigs, 18 MRSA from bovines, 8 MRSA from humans; 1 MSSA from bovine, 1 MSSA from pig, 1 MSSA from ovine, and 6 MSSA from human) and the remainders from Finland (6 MRSA from pigs), Spain (4 MRSA and one MSSA from pigs and 2 MSSA from humans), Denmark (4 MRSA from humans), Cyprus (1 MRSA from pigs).


Livestock-Associated Methicillin Resistant and Methicillin Susceptible Staphylococcus aureus Sequence Type (CC)1 in European Farmed Animals: High Genetic Relatedness of Isolates from Italian Cattle Herds and Humans.

Alba P, Feltrin F, Cordaro G, Porrero MC, Kraushaar B, Argudín MA, Nykäsenoja S, Monaco M, Stegger M, Aarestrup FM, Butaye P, Franco A, Battisti A - PLoS ONE (2015)

Dendrogram of SmaI PFGE macrorestriction patterns, with selected virulence and antimicrobial resistance marker genes and elements of Sequence Type 1, Clonal Complex 1 MSSA and MRSA from animals and humans.Legend: † LukF-PV(P83)-LukM; ‡ vwb (COL+MW2); ☼Ciprofloxacin resistance; § PFGE profile. Notes: *SCCmecV&SCCfus; ** SCCmec IV (2B&5), subtype IVa.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556339&req=5

pone.0137143.g001: Dendrogram of SmaI PFGE macrorestriction patterns, with selected virulence and antimicrobial resistance marker genes and elements of Sequence Type 1, Clonal Complex 1 MSSA and MRSA from animals and humans.Legend: † LukF-PV(P83)-LukM; ‡ vwb (COL+MW2); ☼Ciprofloxacin resistance; § PFGE profile. Notes: *SCCmecV&SCCfus; ** SCCmec IV (2B&5), subtype IVa.
Mentions: A total of 83 ST(CC)1 S. aureus (71 MRSA and 12 MSSA) were studied (Fig 1). The majority (n = 65) were from Italy (including 30 MRSA from pigs, 18 MRSA from bovines, 8 MRSA from humans; 1 MSSA from bovine, 1 MSSA from pig, 1 MSSA from ovine, and 6 MSSA from human) and the remainders from Finland (6 MRSA from pigs), Spain (4 MRSA and one MSSA from pigs and 2 MSSA from humans), Denmark (4 MRSA from humans), Cyprus (1 MRSA from pigs).

Bottom Line: They often showed genetic features typical of human adaptation or present in human-associated CC1: Immune evasion cluster (IEC) genes sak and scn, or sea; sat and aphA3-mediated aminoglycoside resistance.As such, these strains may represent a greater human hazard than the porcine strains.The zoonotic capacity of CC1 LA-MRSA from livestock must be taken seriously and measures should be implemented at farm-level to prevent spill-over.

View Article: PubMed Central - PubMed

Affiliation: Istituto Zooprofilattico Sperimentale del Lazio e della Toscana "M. Aleandri", Diagnostic Department, Rome, Italy.

ABSTRACT
Methicillin-resistant Staphylococcus aureus (MRSA) Sequence Type (ST)1, Clonal Complex(CC)1, SCCmec V is one of the major Livestock-Associated (LA-) lineages in pig farming industry in Italy and is associated with pigs in other European countries. Recently, it has been increasingly detected in Italian dairy cattle herds. The aim of this study was to analyse the differences between ST1 MRSA and methicillin-susceptible S. aureus (MSSA) from cattle and pig herds in Italy and Europe and human isolates. Sixty-tree animal isolates from different holdings and 20 human isolates were characterized by pulsed-field gel electrophoresis (PFGE), spa-typing, SCCmec typing, and by micro-array analysis for several virulence, antimicrobial resistance, and strain/host-specific marker genes. Three major PFGE clusters were detected. The bovine isolates shared a high (≥90% to 100%) similarity with human isolates and carried the same SCCmec type IVa. They often showed genetic features typical of human adaptation or present in human-associated CC1: Immune evasion cluster (IEC) genes sak and scn, or sea; sat and aphA3-mediated aminoglycoside resistance. Contrary, typical markers of porcine origin in Italy and Spain, like erm(A) mediated macrolide-lincosamide-streptograminB, and of vga(A)-mediated pleuromutilin resistance were always absent in human and bovine isolates. Most of ST(CC)1 MRSA from dairy cattle were multidrug-resistant and contained virulence and immunomodulatory genes associated with full capability of colonizing humans. As such, these strains may represent a greater human hazard than the porcine strains. The zoonotic capacity of CC1 LA-MRSA from livestock must be taken seriously and measures should be implemented at farm-level to prevent spill-over.

No MeSH data available.


Related in: MedlinePlus