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In Vivo Delivery of Tinospora cordifolia Root Extract Preventing Radiation-Induced Dystrophies in Mice Ovaries.

Sharma R - Evid Based Complement Alternat Med (2015)

Bottom Line: The present study aims at finding radioprotective effect of Tinospora cordifolia (Willd.) Miers root extract (TCRE) in ovaries.To understand the mechanism of radioprotection, lipid peroxidation (LPO) and glutathione (GSH) levels were also measured in all groups.TCRE supplementation rendered significant protection to ovaries by restoring follicle counts; it also reduced LPO levels and increased GSH levels in ovaries.

View Article: PubMed Central - PubMed

Affiliation: Amity Education Valley, Kant Kalwar, NH-11C, Jaipur Delhi Highway, Jaipur, Rajasthan 303007, India ; Department of Zoology, University of Rajasthan, JLN Marg, Jaipur, Rajasthan 302004, India.

ABSTRACT
Unconscious and unplanned radiation exposures are a severe threat to gonads particularly ovaries. The present study aims at finding radioprotective effect of Tinospora cordifolia (Willd.) Miers root extract (TCRE) in ovaries. Swiss albino mice were divided into four groups: Group 1 served as "normal" and is administered double distilled water and Group 2 is given TCRE with optimum dosage selected as 75 mg/mice. Group 3 serving the purpose of "irradiated control" were exposed to 2.5 Gy gamma radiation. Group 4 (experimental) were administered optimum dosage of TCRE with prior exposure to 2.5 Gy gamma radiation. Follicle cell counts were scored at autopsy intervals of 24 hrs, 3 days, 7 days, 15 days, and 30 days after gamma irradiation. To understand the mechanism of radioprotection, lipid peroxidation (LPO) and glutathione (GSH) levels were also measured in all groups. TCRE supplementation rendered significant protection to ovaries by restoring follicle counts; it also reduced LPO levels and increased GSH levels in ovaries. It implies that TCRE administration protects ovaries against radiation exposure.

No MeSH data available.


Related in: MedlinePlus

Follicle cell population at various intervals after exposure to 2.5 Gy gamma radiation with (experimental) or without (irradiated control) TCRE.
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Related In: Results  -  Collection


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fig2: Follicle cell population at various intervals after exposure to 2.5 Gy gamma radiation with (experimental) or without (irradiated control) TCRE.

Mentions: In Group 3 there is “p ≤ 0.001” significant decrease in follicle cell population comparable to normal (Group 1) for category “A.” Similarly, follicle cell population continues to decrease (p ≤ 0.001) till 30th day in Group 3 for categories “B” and “C.” In TCRE treated animals (Group 4) for category “A,” there is 8.47% (p ≤ 0.001) increase in number of follicles comparable to Group 3 at 24 hrs. Similar restorative behavior is being experienced by categories “B” and “C” as shown in Figure 2.


In Vivo Delivery of Tinospora cordifolia Root Extract Preventing Radiation-Induced Dystrophies in Mice Ovaries.

Sharma R - Evid Based Complement Alternat Med (2015)

Follicle cell population at various intervals after exposure to 2.5 Gy gamma radiation with (experimental) or without (irradiated control) TCRE.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4556323&req=5

fig2: Follicle cell population at various intervals after exposure to 2.5 Gy gamma radiation with (experimental) or without (irradiated control) TCRE.
Mentions: In Group 3 there is “p ≤ 0.001” significant decrease in follicle cell population comparable to normal (Group 1) for category “A.” Similarly, follicle cell population continues to decrease (p ≤ 0.001) till 30th day in Group 3 for categories “B” and “C.” In TCRE treated animals (Group 4) for category “A,” there is 8.47% (p ≤ 0.001) increase in number of follicles comparable to Group 3 at 24 hrs. Similar restorative behavior is being experienced by categories “B” and “C” as shown in Figure 2.

Bottom Line: The present study aims at finding radioprotective effect of Tinospora cordifolia (Willd.) Miers root extract (TCRE) in ovaries.To understand the mechanism of radioprotection, lipid peroxidation (LPO) and glutathione (GSH) levels were also measured in all groups.TCRE supplementation rendered significant protection to ovaries by restoring follicle counts; it also reduced LPO levels and increased GSH levels in ovaries.

View Article: PubMed Central - PubMed

Affiliation: Amity Education Valley, Kant Kalwar, NH-11C, Jaipur Delhi Highway, Jaipur, Rajasthan 303007, India ; Department of Zoology, University of Rajasthan, JLN Marg, Jaipur, Rajasthan 302004, India.

ABSTRACT
Unconscious and unplanned radiation exposures are a severe threat to gonads particularly ovaries. The present study aims at finding radioprotective effect of Tinospora cordifolia (Willd.) Miers root extract (TCRE) in ovaries. Swiss albino mice were divided into four groups: Group 1 served as "normal" and is administered double distilled water and Group 2 is given TCRE with optimum dosage selected as 75 mg/mice. Group 3 serving the purpose of "irradiated control" were exposed to 2.5 Gy gamma radiation. Group 4 (experimental) were administered optimum dosage of TCRE with prior exposure to 2.5 Gy gamma radiation. Follicle cell counts were scored at autopsy intervals of 24 hrs, 3 days, 7 days, 15 days, and 30 days after gamma irradiation. To understand the mechanism of radioprotection, lipid peroxidation (LPO) and glutathione (GSH) levels were also measured in all groups. TCRE supplementation rendered significant protection to ovaries by restoring follicle counts; it also reduced LPO levels and increased GSH levels in ovaries. It implies that TCRE administration protects ovaries against radiation exposure.

No MeSH data available.


Related in: MedlinePlus