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Viral infections in prostate carcinomas in Chilean patients.

Rodríguez H, Levican J, Muñoz JP, Carrillo D, Acevedo ML, Gaggero A, León O, Gheit T, Espinoza-Navarro O, Castillo J, Gallegos I, Tommasino M, Aguayo F - Infect. Agents Cancer (2015)

Bottom Line: HPV and JCPyV were not detected in any specimens (0/69, 0 %); whereas, BKPyV was detected in 6/69 PCas (8.7 %).We did not find a statistically significant association between the presence of BKPyV and age (p = 0.198) or Gleason score (p = 0.268).In addition, 2/6 (33 %) BKPyV positive specimens showed detectable levels of TAg transcripts.

View Article: PubMed Central - PubMed

Affiliation: Anatomy and Development Biology Program, Institute of Biomedical Sciences (ICBM), Faculty of Medicine, Universidad de Chile, Santiago, Chile.

ABSTRACT

Background: A few viruses have been detected in prostate cancer, however their role in the development of this malignancy has not been determined. The aim of this study was to analyze the presence and functionality of human papillomavirus (HPV) and polyomaviruses (BKPyV and JCPyV) in prostate carcinomas in Chilean patients.

Methods: Sixty-nine primary prostate carcinomas were analyzed for the presence of HPV, BKPyV and JCPyV using standard polymerase chain reaction protocols. In addition, when samples were positive for HPyV, large T antigen (TAg) transcripts were analyzed using reverse transcriptase PCR.

Results: HPV and JCPyV were not detected in any specimens (0/69, 0 %); whereas, BKPyV was detected in 6/69 PCas (8.7 %). We did not find a statistically significant association between the presence of BKPyV and age (p = 0.198) or Gleason score (p = 0.268). In addition, 2/6 (33 %) BKPyV positive specimens showed detectable levels of TAg transcripts.

Conclusions: There was no association between HPV or JCPyV presence and prostate cancer development. The presence of BKPyV in a small subset of prostate carcinomas in Chilean patients could indicate that this virus plays a potential role in prostate cancer development and requires further investigation.

No MeSH data available.


Related in: MedlinePlus

Dissociation curves and melting analysis of BKPyV positive and negative cases. The observed Tm was 80.8ºC.
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Fig1: Dissociation curves and melting analysis of BKPyV positive and negative cases. The observed Tm was 80.8ºC.

Mentions: Amplification of a 110 bp fragment of b-globin gave a similar yield in all samples. Using a highly sensitive genotyping assay (TS-MPG), HPV genomes were not detected in any of the samples. In addition, the absence of HPV genomes in all samples was confirmed by an independent GP5+/6+ PCR assay [26]. In contrast, for polyomaviruses we found that 6/69 (8.7 %) samples were positive by qPCR for BKPyV. Table 2 shows BKPyV detection and its association with clinical-pathological features. No statistically significant differences were found between age (p = 0.198), Gleason score (p = 0.268) or hospital (p = 0.402) and BKPyV presence in PCa. The dissociation curves for BKPyV and melting analysis of BKPyV positive and negative cases are shown in the Fig. 1.Table 2


Viral infections in prostate carcinomas in Chilean patients.

Rodríguez H, Levican J, Muñoz JP, Carrillo D, Acevedo ML, Gaggero A, León O, Gheit T, Espinoza-Navarro O, Castillo J, Gallegos I, Tommasino M, Aguayo F - Infect. Agents Cancer (2015)

Dissociation curves and melting analysis of BKPyV positive and negative cases. The observed Tm was 80.8ºC.
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4556319&req=5

Fig1: Dissociation curves and melting analysis of BKPyV positive and negative cases. The observed Tm was 80.8ºC.
Mentions: Amplification of a 110 bp fragment of b-globin gave a similar yield in all samples. Using a highly sensitive genotyping assay (TS-MPG), HPV genomes were not detected in any of the samples. In addition, the absence of HPV genomes in all samples was confirmed by an independent GP5+/6+ PCR assay [26]. In contrast, for polyomaviruses we found that 6/69 (8.7 %) samples were positive by qPCR for BKPyV. Table 2 shows BKPyV detection and its association with clinical-pathological features. No statistically significant differences were found between age (p = 0.198), Gleason score (p = 0.268) or hospital (p = 0.402) and BKPyV presence in PCa. The dissociation curves for BKPyV and melting analysis of BKPyV positive and negative cases are shown in the Fig. 1.Table 2

Bottom Line: HPV and JCPyV were not detected in any specimens (0/69, 0 %); whereas, BKPyV was detected in 6/69 PCas (8.7 %).We did not find a statistically significant association between the presence of BKPyV and age (p = 0.198) or Gleason score (p = 0.268).In addition, 2/6 (33 %) BKPyV positive specimens showed detectable levels of TAg transcripts.

View Article: PubMed Central - PubMed

Affiliation: Anatomy and Development Biology Program, Institute of Biomedical Sciences (ICBM), Faculty of Medicine, Universidad de Chile, Santiago, Chile.

ABSTRACT

Background: A few viruses have been detected in prostate cancer, however their role in the development of this malignancy has not been determined. The aim of this study was to analyze the presence and functionality of human papillomavirus (HPV) and polyomaviruses (BKPyV and JCPyV) in prostate carcinomas in Chilean patients.

Methods: Sixty-nine primary prostate carcinomas were analyzed for the presence of HPV, BKPyV and JCPyV using standard polymerase chain reaction protocols. In addition, when samples were positive for HPyV, large T antigen (TAg) transcripts were analyzed using reverse transcriptase PCR.

Results: HPV and JCPyV were not detected in any specimens (0/69, 0 %); whereas, BKPyV was detected in 6/69 PCas (8.7 %). We did not find a statistically significant association between the presence of BKPyV and age (p = 0.198) or Gleason score (p = 0.268). In addition, 2/6 (33 %) BKPyV positive specimens showed detectable levels of TAg transcripts.

Conclusions: There was no association between HPV or JCPyV presence and prostate cancer development. The presence of BKPyV in a small subset of prostate carcinomas in Chilean patients could indicate that this virus plays a potential role in prostate cancer development and requires further investigation.

No MeSH data available.


Related in: MedlinePlus