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Enhanced detection sensitivity of Escherichia coli O157:H7 using surface-modified gold nanorods.

Ramasamy M, Yi DK, An SS - Int J Nanomedicine (2015)

Bottom Line: Endogenous membrane peroxidase, an enzyme present on the surface of O157, was used for the colorimetric detection of bacteria by catalytic oxidation of the peroxidase substrate.In this study, we have analyzed the impact of the synthesized bare gold nanorods (AuNRs) and silica-coated AuNRs on the growth of E. coli O157.This result can be particularly important for the enzymatic analysis of surface treated AuNRs in various microbiological applicants.

View Article: PubMed Central - PubMed

Affiliation: School of Chemical Engineering, Yeungnam University, Gyeongsan, Republic of Korea.

ABSTRACT
Escherichia coli O157:H7 (O157) is a Gram negative and highly virulent bacteria found in food and water sources, and is a leading cause of chronic diseases worldwide. Diagnosis and prevention from the infection require simple and rapid analysis methods for the detection of pathogens, including O157. Endogenous membrane peroxidase, an enzyme present on the surface of O157, was used for the colorimetric detection of bacteria by catalytic oxidation of the peroxidase substrate. In this study, we have analyzed the impact of the synthesized bare gold nanorods (AuNRs) and silica-coated AuNRs on the growth of E. coli O157. Along with the membrane peroxidase activity of O157, other bacteria strains were analyzed. Different concentrations of nanorods were used to analyze the growth responses, enzymatic changes, and morphological alterations of bacteria by measuring optical density, 3,3',5,5'-tetramethylbenzidine assay, flow cytometry analysis, and microscopy studies. The results revealed that O157 showed higher and continuous membrane peroxidase activity than other bacteria. Furthermore, O157 treated with bare AuNRs showed a decreased growth rate in comparison with the bacteria with surface modified AuNRs. Interestingly, silica-coated AuNRs favored the growth of bacteria and also increased membrane peroxidase activity. This result can be particularly important for the enzymatic analysis of surface treated AuNRs in various microbiological applicants.

No MeSH data available.


Related in: MedlinePlus

Bacterial dynamic growth curve of E. coli O157, E. faecalis, B. subtilis, and S. typhi in LB medium.Abbreviations: OD, optical density; LB, Lysogeny Broth; E. coli O157, Escherichia coli O157; E. faecalis, Enterococcus faecalis; B. subtilis, Bacillus subtilis; S. typhi, Salmonella typhimurium.
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f2-ijn-10-179: Bacterial dynamic growth curve of E. coli O157, E. faecalis, B. subtilis, and S. typhi in LB medium.Abbreviations: OD, optical density; LB, Lysogeny Broth; E. coli O157, Escherichia coli O157; E. faecalis, Enterococcus faecalis; B. subtilis, Bacillus subtilis; S. typhi, Salmonella typhimurium.

Mentions: Comparison among the regular growth rate of different bacterial cells is shown in Figure 2. The obtained results showed that at 10 hours, B. subtilis showed higher growth, and E. coli O157 showed slower growth compared to E. faecalis and S. typhi, which showed normal growth rate at the same incubation conditions. Since all bacteria showed similar growth phase, these bacteria were used to analyze EMP activities.


Enhanced detection sensitivity of Escherichia coli O157:H7 using surface-modified gold nanorods.

Ramasamy M, Yi DK, An SS - Int J Nanomedicine (2015)

Bacterial dynamic growth curve of E. coli O157, E. faecalis, B. subtilis, and S. typhi in LB medium.Abbreviations: OD, optical density; LB, Lysogeny Broth; E. coli O157, Escherichia coli O157; E. faecalis, Enterococcus faecalis; B. subtilis, Bacillus subtilis; S. typhi, Salmonella typhimurium.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556300&req=5

f2-ijn-10-179: Bacterial dynamic growth curve of E. coli O157, E. faecalis, B. subtilis, and S. typhi in LB medium.Abbreviations: OD, optical density; LB, Lysogeny Broth; E. coli O157, Escherichia coli O157; E. faecalis, Enterococcus faecalis; B. subtilis, Bacillus subtilis; S. typhi, Salmonella typhimurium.
Mentions: Comparison among the regular growth rate of different bacterial cells is shown in Figure 2. The obtained results showed that at 10 hours, B. subtilis showed higher growth, and E. coli O157 showed slower growth compared to E. faecalis and S. typhi, which showed normal growth rate at the same incubation conditions. Since all bacteria showed similar growth phase, these bacteria were used to analyze EMP activities.

Bottom Line: Endogenous membrane peroxidase, an enzyme present on the surface of O157, was used for the colorimetric detection of bacteria by catalytic oxidation of the peroxidase substrate.In this study, we have analyzed the impact of the synthesized bare gold nanorods (AuNRs) and silica-coated AuNRs on the growth of E. coli O157.This result can be particularly important for the enzymatic analysis of surface treated AuNRs in various microbiological applicants.

View Article: PubMed Central - PubMed

Affiliation: School of Chemical Engineering, Yeungnam University, Gyeongsan, Republic of Korea.

ABSTRACT
Escherichia coli O157:H7 (O157) is a Gram negative and highly virulent bacteria found in food and water sources, and is a leading cause of chronic diseases worldwide. Diagnosis and prevention from the infection require simple and rapid analysis methods for the detection of pathogens, including O157. Endogenous membrane peroxidase, an enzyme present on the surface of O157, was used for the colorimetric detection of bacteria by catalytic oxidation of the peroxidase substrate. In this study, we have analyzed the impact of the synthesized bare gold nanorods (AuNRs) and silica-coated AuNRs on the growth of E. coli O157. Along with the membrane peroxidase activity of O157, other bacteria strains were analyzed. Different concentrations of nanorods were used to analyze the growth responses, enzymatic changes, and morphological alterations of bacteria by measuring optical density, 3,3',5,5'-tetramethylbenzidine assay, flow cytometry analysis, and microscopy studies. The results revealed that O157 showed higher and continuous membrane peroxidase activity than other bacteria. Furthermore, O157 treated with bare AuNRs showed a decreased growth rate in comparison with the bacteria with surface modified AuNRs. Interestingly, silica-coated AuNRs favored the growth of bacteria and also increased membrane peroxidase activity. This result can be particularly important for the enzymatic analysis of surface treated AuNRs in various microbiological applicants.

No MeSH data available.


Related in: MedlinePlus