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Anti-Oxidative Effect of Myrtenal in Prevention and Treatment of Colon Cancer Induced by 1, 2-Dimethyl Hydrazine (DMH) in Experimental Animals.

Lokeshkumar B, Sathishkumar V, Nandakumar N, Rengarajan T, Madankumar A, Balasubramanian MP - Biomol Ther (Seoul) (2015)

Bottom Line: Monoterpenes are a major part of plant essential oils found largely in fruits, vegetables and herbs.The Invivo results exhibit the elevated antioxidant and lipid peroxidation levels in DMH treated animals.It is thus concluded that myrtenal exhibits excellent free radical scavenging activity and anticancer activity through the suppression of colon carcinoma in Wistar albino rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology and Environmental Toxicology, Dr. A. L. MPGIBMS, University of Madras, Taramani Campus, Chennai- 600 113, Tamilnadu, India.

ABSTRACT
Colon cancer is considered as the precarious forms of cancer in many developed countries, with few to no symptoms; the tumor is often diagnosed in the later stages of cancer. Monoterpenes are a major part of plant essential oils found largely in fruits, vegetables and herbs. The cellular and molecular activities show therapeutic progression that may reduce the risk of developing cancer by modulating the factors responsible for colon carcinogenesis. Colon cancer was induced with DMH with a dose of (20 mg/Kg/body weight) for 15 weeks by subcutaneous injection once in a week. Myrtenal treatment was started with (230 mg/Kg/body weight) by intragastric administration, one week prior to DMH induction and continued till the experimental period of 30 weeks. The Invivo results exhibit the elevated antioxidant and lipid peroxidation levels in DMH treated animals. The Histopathological analysis of colon tissues well supported the biochemical alterations and inevitably proves the protective role of Myrtenal. Treatment with myrtenal to cancer bearing animals resulted in a remarkable increase in the inherent antioxidants and excellent modulation in the morphological and physiological nature of the colon tissue. It is thus concluded that myrtenal exhibits excellent free radical scavenging activity and anticancer activity through the suppression of colon carcinoma in Wistar albino rats.

No MeSH data available.


Related in: MedlinePlus

Effect of myrtenal on MDA levels in colon tissue of control and experimental animals. Group I control animals express normal levels of MDA, a biomarker for lipid peroxidation. Group II DMH induced animals show increased MDA levels as a result of lipid peroxidation and enhanced cell division by DNA methylating adducts by DMH. Group III Myrtenal treated animals express significant reduction in the levels of MDA as a result of gradual regeneration of the colon cells. Group IV Myrtenal alone treated animals posses no significant changes expressing no cytotoxic effect on the experimental animals. Results are expressed as mean ± S.D for six animals. aGroup II, III & IV compared with Group I, bGroup III compared with Group II,*p<0.05, NSNot significant.
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f1-bt-23-471: Effect of myrtenal on MDA levels in colon tissue of control and experimental animals. Group I control animals express normal levels of MDA, a biomarker for lipid peroxidation. Group II DMH induced animals show increased MDA levels as a result of lipid peroxidation and enhanced cell division by DNA methylating adducts by DMH. Group III Myrtenal treated animals express significant reduction in the levels of MDA as a result of gradual regeneration of the colon cells. Group IV Myrtenal alone treated animals posses no significant changes expressing no cytotoxic effect on the experimental animals. Results are expressed as mean ± S.D for six animals. aGroup II, III & IV compared with Group I, bGroup III compared with Group II,*p<0.05, NSNot significant.

Mentions: In our study, MDA levels were found to be significantly increased in group II cancer bearing animals compared to group I control animals (Fig. 1). Myrtenal administration significantly reduced the peroxidation reaction in group III animals compared with group II cancer bearing animals. However, no significant changes were observed in group IV myrtenal alone treated animals. Enzymic and non-enzymic antioxidant levels are shown in Fig. 2 with a significant reduction of SOD, CAT, GPX, and GR activities in DMH alone-treated group II animals as compared to the control. But on myrtenal supplementation in group III the decreased levels were significantly elevated as compared to the DMH-treated group II (Fig. 2). The concentrations of α-tocopherol and vitamin C were reduced in DMH treated group II cancer bearing animals as compared to group I control animals. But on Myrtenal supplementation, the concentration of α-tocopherol and vitamin C were significantly increased (p<0.05) as compared to the DMH-treated group II animals. No significant changes were observed in group IV myrtenal alone treated animals.


Anti-Oxidative Effect of Myrtenal in Prevention and Treatment of Colon Cancer Induced by 1, 2-Dimethyl Hydrazine (DMH) in Experimental Animals.

Lokeshkumar B, Sathishkumar V, Nandakumar N, Rengarajan T, Madankumar A, Balasubramanian MP - Biomol Ther (Seoul) (2015)

Effect of myrtenal on MDA levels in colon tissue of control and experimental animals. Group I control animals express normal levels of MDA, a biomarker for lipid peroxidation. Group II DMH induced animals show increased MDA levels as a result of lipid peroxidation and enhanced cell division by DNA methylating adducts by DMH. Group III Myrtenal treated animals express significant reduction in the levels of MDA as a result of gradual regeneration of the colon cells. Group IV Myrtenal alone treated animals posses no significant changes expressing no cytotoxic effect on the experimental animals. Results are expressed as mean ± S.D for six animals. aGroup II, III & IV compared with Group I, bGroup III compared with Group II,*p<0.05, NSNot significant.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556208&req=5

f1-bt-23-471: Effect of myrtenal on MDA levels in colon tissue of control and experimental animals. Group I control animals express normal levels of MDA, a biomarker for lipid peroxidation. Group II DMH induced animals show increased MDA levels as a result of lipid peroxidation and enhanced cell division by DNA methylating adducts by DMH. Group III Myrtenal treated animals express significant reduction in the levels of MDA as a result of gradual regeneration of the colon cells. Group IV Myrtenal alone treated animals posses no significant changes expressing no cytotoxic effect on the experimental animals. Results are expressed as mean ± S.D for six animals. aGroup II, III & IV compared with Group I, bGroup III compared with Group II,*p<0.05, NSNot significant.
Mentions: In our study, MDA levels were found to be significantly increased in group II cancer bearing animals compared to group I control animals (Fig. 1). Myrtenal administration significantly reduced the peroxidation reaction in group III animals compared with group II cancer bearing animals. However, no significant changes were observed in group IV myrtenal alone treated animals. Enzymic and non-enzymic antioxidant levels are shown in Fig. 2 with a significant reduction of SOD, CAT, GPX, and GR activities in DMH alone-treated group II animals as compared to the control. But on myrtenal supplementation in group III the decreased levels were significantly elevated as compared to the DMH-treated group II (Fig. 2). The concentrations of α-tocopherol and vitamin C were reduced in DMH treated group II cancer bearing animals as compared to group I control animals. But on Myrtenal supplementation, the concentration of α-tocopherol and vitamin C were significantly increased (p<0.05) as compared to the DMH-treated group II animals. No significant changes were observed in group IV myrtenal alone treated animals.

Bottom Line: Monoterpenes are a major part of plant essential oils found largely in fruits, vegetables and herbs.The Invivo results exhibit the elevated antioxidant and lipid peroxidation levels in DMH treated animals.It is thus concluded that myrtenal exhibits excellent free radical scavenging activity and anticancer activity through the suppression of colon carcinoma in Wistar albino rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology and Environmental Toxicology, Dr. A. L. MPGIBMS, University of Madras, Taramani Campus, Chennai- 600 113, Tamilnadu, India.

ABSTRACT
Colon cancer is considered as the precarious forms of cancer in many developed countries, with few to no symptoms; the tumor is often diagnosed in the later stages of cancer. Monoterpenes are a major part of plant essential oils found largely in fruits, vegetables and herbs. The cellular and molecular activities show therapeutic progression that may reduce the risk of developing cancer by modulating the factors responsible for colon carcinogenesis. Colon cancer was induced with DMH with a dose of (20 mg/Kg/body weight) for 15 weeks by subcutaneous injection once in a week. Myrtenal treatment was started with (230 mg/Kg/body weight) by intragastric administration, one week prior to DMH induction and continued till the experimental period of 30 weeks. The Invivo results exhibit the elevated antioxidant and lipid peroxidation levels in DMH treated animals. The Histopathological analysis of colon tissues well supported the biochemical alterations and inevitably proves the protective role of Myrtenal. Treatment with myrtenal to cancer bearing animals resulted in a remarkable increase in the inherent antioxidants and excellent modulation in the morphological and physiological nature of the colon tissue. It is thus concluded that myrtenal exhibits excellent free radical scavenging activity and anticancer activity through the suppression of colon carcinoma in Wistar albino rats.

No MeSH data available.


Related in: MedlinePlus