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Chondroprotective Effects of Wogonin in Experimental Models of Osteoarthritis in vitro and in vivo.

Park JS, Lee HJ, Lee DY, Jo HS, Jeong JH, Kim DH, Nam DC, Lee CJ, Hwang SC - Biomol Ther (Seoul) (2015)

Bottom Line: In rabbit articular chondrocytes, the effects of wogonin on IL-1β-induced production and proteolytic activity of MMP-3 were investigated using western blot analysis and casein zymography, respectively.The effect of wogonin on MMP-3 protein production was also examined in vivo.These results suggest that wogonin can regulate the gene expression and production of MMP-3, by directly acting on articular chondrocytes.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery and Institute of Health Sciences, School of Medicine and Hospital, Gyeongsang National University, Jinju 660-701, Republic of Korea.

ABSTRACT
We evaluated the chondroprotective effects of wogonin by investigating its effects on the gene expression and production of matrix metalloproteinase-3 (MMP-3) in primary cultured rabbit articular chondrocytes, as well as on production of MMP-3 in the rat knee. Rabbit articular chondrocytes were cultured in a monolayer, and RT-PCR was used to measure interleukin-1β (IL-1β)-induced expression of MMP-3, MMP-1, MMP-13, a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4), and type II collagen. In rabbit articular chondrocytes, the effects of wogonin on IL-1β-induced production and proteolytic activity of MMP-3 were investigated using western blot analysis and casein zymography, respectively. The effect of wogonin on MMP-3 protein production was also examined in vivo. In rabbit articular chondrocytes, wogonin inhibited the expression of MMP-3, MMP-1, MMP-13, and ADAMTS-4, but increased expression of type II collagen. Furthermore, wogonin inhibited the production and proteolytic activity of MMP-3 in vitro, and inhibited production of MMP-3 protein in vivo. These results suggest that wogonin can regulate the gene expression and production of MMP-3, by directly acting on articular chondrocytes.

No MeSH data available.


Effect of wogonin on IL-1β-induced production of MMP-3 from rabbit articular chondrocytes. Primary cultured rabbit articular chondrocytes were pretreated with varying concentrations (1, 10, 50, and 100 μM) of wogonin for 2 h and then stimulated with IL-1β (10 ng/mL) for 24 h. Culture supernatants were collected for measurement of the levels of produced MMP-3 by western blot analysis. Three independent experiments were performed and the representative data were shown (cont: control, concentration unit is μM). *Significantly different from control (p<0.05). †Significantly different from IL-1β alone (p<0.05).
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f5-bt-23-442: Effect of wogonin on IL-1β-induced production of MMP-3 from rabbit articular chondrocytes. Primary cultured rabbit articular chondrocytes were pretreated with varying concentrations (1, 10, 50, and 100 μM) of wogonin for 2 h and then stimulated with IL-1β (10 ng/mL) for 24 h. Culture supernatants were collected for measurement of the levels of produced MMP-3 by western blot analysis. Three independent experiments were performed and the representative data were shown (cont: control, concentration unit is μM). *Significantly different from control (p<0.05). †Significantly different from IL-1β alone (p<0.05).

Mentions: If wogonin can affect the MMP-3 gene expression at the transcriptional level, it should be investigated whether wogonin affects IL-1β-induced production of MMP-3 proteins from rabbit articular chondrocytes. As shown in Fig. 5, stimulation with IL-1β (10 ng/mL) increased production of MMP-3 from chondrocytes. However, wogonin inhibited the effects of IL-1β on MMP-3 production. This result means that wogonin can control the steps of protein synthesis (production) of MMP-3.


Chondroprotective Effects of Wogonin in Experimental Models of Osteoarthritis in vitro and in vivo.

Park JS, Lee HJ, Lee DY, Jo HS, Jeong JH, Kim DH, Nam DC, Lee CJ, Hwang SC - Biomol Ther (Seoul) (2015)

Effect of wogonin on IL-1β-induced production of MMP-3 from rabbit articular chondrocytes. Primary cultured rabbit articular chondrocytes were pretreated with varying concentrations (1, 10, 50, and 100 μM) of wogonin for 2 h and then stimulated with IL-1β (10 ng/mL) for 24 h. Culture supernatants were collected for measurement of the levels of produced MMP-3 by western blot analysis. Three independent experiments were performed and the representative data were shown (cont: control, concentration unit is μM). *Significantly different from control (p<0.05). †Significantly different from IL-1β alone (p<0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556204&req=5

f5-bt-23-442: Effect of wogonin on IL-1β-induced production of MMP-3 from rabbit articular chondrocytes. Primary cultured rabbit articular chondrocytes were pretreated with varying concentrations (1, 10, 50, and 100 μM) of wogonin for 2 h and then stimulated with IL-1β (10 ng/mL) for 24 h. Culture supernatants were collected for measurement of the levels of produced MMP-3 by western blot analysis. Three independent experiments were performed and the representative data were shown (cont: control, concentration unit is μM). *Significantly different from control (p<0.05). †Significantly different from IL-1β alone (p<0.05).
Mentions: If wogonin can affect the MMP-3 gene expression at the transcriptional level, it should be investigated whether wogonin affects IL-1β-induced production of MMP-3 proteins from rabbit articular chondrocytes. As shown in Fig. 5, stimulation with IL-1β (10 ng/mL) increased production of MMP-3 from chondrocytes. However, wogonin inhibited the effects of IL-1β on MMP-3 production. This result means that wogonin can control the steps of protein synthesis (production) of MMP-3.

Bottom Line: In rabbit articular chondrocytes, the effects of wogonin on IL-1β-induced production and proteolytic activity of MMP-3 were investigated using western blot analysis and casein zymography, respectively.The effect of wogonin on MMP-3 protein production was also examined in vivo.These results suggest that wogonin can regulate the gene expression and production of MMP-3, by directly acting on articular chondrocytes.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery and Institute of Health Sciences, School of Medicine and Hospital, Gyeongsang National University, Jinju 660-701, Republic of Korea.

ABSTRACT
We evaluated the chondroprotective effects of wogonin by investigating its effects on the gene expression and production of matrix metalloproteinase-3 (MMP-3) in primary cultured rabbit articular chondrocytes, as well as on production of MMP-3 in the rat knee. Rabbit articular chondrocytes were cultured in a monolayer, and RT-PCR was used to measure interleukin-1β (IL-1β)-induced expression of MMP-3, MMP-1, MMP-13, a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4), and type II collagen. In rabbit articular chondrocytes, the effects of wogonin on IL-1β-induced production and proteolytic activity of MMP-3 were investigated using western blot analysis and casein zymography, respectively. The effect of wogonin on MMP-3 protein production was also examined in vivo. In rabbit articular chondrocytes, wogonin inhibited the expression of MMP-3, MMP-1, MMP-13, and ADAMTS-4, but increased expression of type II collagen. Furthermore, wogonin inhibited the production and proteolytic activity of MMP-3 in vitro, and inhibited production of MMP-3 protein in vivo. These results suggest that wogonin can regulate the gene expression and production of MMP-3, by directly acting on articular chondrocytes.

No MeSH data available.