Limits...
Chondroprotective Effects of Wogonin in Experimental Models of Osteoarthritis in vitro and in vivo.

Park JS, Lee HJ, Lee DY, Jo HS, Jeong JH, Kim DH, Nam DC, Lee CJ, Hwang SC - Biomol Ther (Seoul) (2015)

Bottom Line: In rabbit articular chondrocytes, the effects of wogonin on IL-1β-induced production and proteolytic activity of MMP-3 were investigated using western blot analysis and casein zymography, respectively.The effect of wogonin on MMP-3 protein production was also examined in vivo.These results suggest that wogonin can regulate the gene expression and production of MMP-3, by directly acting on articular chondrocytes.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery and Institute of Health Sciences, School of Medicine and Hospital, Gyeongsang National University, Jinju 660-701, Republic of Korea.

ABSTRACT
We evaluated the chondroprotective effects of wogonin by investigating its effects on the gene expression and production of matrix metalloproteinase-3 (MMP-3) in primary cultured rabbit articular chondrocytes, as well as on production of MMP-3 in the rat knee. Rabbit articular chondrocytes were cultured in a monolayer, and RT-PCR was used to measure interleukin-1β (IL-1β)-induced expression of MMP-3, MMP-1, MMP-13, a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4), and type II collagen. In rabbit articular chondrocytes, the effects of wogonin on IL-1β-induced production and proteolytic activity of MMP-3 were investigated using western blot analysis and casein zymography, respectively. The effect of wogonin on MMP-3 protein production was also examined in vivo. In rabbit articular chondrocytes, wogonin inhibited the expression of MMP-3, MMP-1, MMP-13, and ADAMTS-4, but increased expression of type II collagen. Furthermore, wogonin inhibited the production and proteolytic activity of MMP-3 in vitro, and inhibited production of MMP-3 protein in vivo. These results suggest that wogonin can regulate the gene expression and production of MMP-3, by directly acting on articular chondrocytes.

No MeSH data available.


Related in: MedlinePlus

Effect of wogonin on proliferation of rabbit chondrocytes. Chondrocytes were incubated for 72 h in the presence of varying concentrations of wogonin. Cell viability was determined using SRB assay as described in Materials and Methods. Each bar represents a mean ± S.E.M. of three independent experiments in comparison with that of the control set at 100%.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4556204&req=5

f3-bt-23-442: Effect of wogonin on proliferation of rabbit chondrocytes. Chondrocytes were incubated for 72 h in the presence of varying concentrations of wogonin. Cell viability was determined using SRB assay as described in Materials and Methods. Each bar represents a mean ± S.E.M. of three independent experiments in comparison with that of the control set at 100%.

Mentions: To check the potential cytotoxicity of wogonin to cultured rabbit chondrocytes, effect of wogonin on proliferation of rabbit chondrocytes using SRB assay. As can be seen in Fig. 3, wogonin showed no significant cytotoxicity at the concentrations of 1, 10, 50, and 100 μM. The numbers of cells in wogonin-treated cultures were 100 ± 15%, 102 ± 13%, 92 ± 16%, 91 ± 14%, and 85 ± 11% for control, 1, 10, 50, and 100 μM wogonin, respectively.


Chondroprotective Effects of Wogonin in Experimental Models of Osteoarthritis in vitro and in vivo.

Park JS, Lee HJ, Lee DY, Jo HS, Jeong JH, Kim DH, Nam DC, Lee CJ, Hwang SC - Biomol Ther (Seoul) (2015)

Effect of wogonin on proliferation of rabbit chondrocytes. Chondrocytes were incubated for 72 h in the presence of varying concentrations of wogonin. Cell viability was determined using SRB assay as described in Materials and Methods. Each bar represents a mean ± S.E.M. of three independent experiments in comparison with that of the control set at 100%.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556204&req=5

f3-bt-23-442: Effect of wogonin on proliferation of rabbit chondrocytes. Chondrocytes were incubated for 72 h in the presence of varying concentrations of wogonin. Cell viability was determined using SRB assay as described in Materials and Methods. Each bar represents a mean ± S.E.M. of three independent experiments in comparison with that of the control set at 100%.
Mentions: To check the potential cytotoxicity of wogonin to cultured rabbit chondrocytes, effect of wogonin on proliferation of rabbit chondrocytes using SRB assay. As can be seen in Fig. 3, wogonin showed no significant cytotoxicity at the concentrations of 1, 10, 50, and 100 μM. The numbers of cells in wogonin-treated cultures were 100 ± 15%, 102 ± 13%, 92 ± 16%, 91 ± 14%, and 85 ± 11% for control, 1, 10, 50, and 100 μM wogonin, respectively.

Bottom Line: In rabbit articular chondrocytes, the effects of wogonin on IL-1β-induced production and proteolytic activity of MMP-3 were investigated using western blot analysis and casein zymography, respectively.The effect of wogonin on MMP-3 protein production was also examined in vivo.These results suggest that wogonin can regulate the gene expression and production of MMP-3, by directly acting on articular chondrocytes.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery and Institute of Health Sciences, School of Medicine and Hospital, Gyeongsang National University, Jinju 660-701, Republic of Korea.

ABSTRACT
We evaluated the chondroprotective effects of wogonin by investigating its effects on the gene expression and production of matrix metalloproteinase-3 (MMP-3) in primary cultured rabbit articular chondrocytes, as well as on production of MMP-3 in the rat knee. Rabbit articular chondrocytes were cultured in a monolayer, and RT-PCR was used to measure interleukin-1β (IL-1β)-induced expression of MMP-3, MMP-1, MMP-13, a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4), and type II collagen. In rabbit articular chondrocytes, the effects of wogonin on IL-1β-induced production and proteolytic activity of MMP-3 were investigated using western blot analysis and casein zymography, respectively. The effect of wogonin on MMP-3 protein production was also examined in vivo. In rabbit articular chondrocytes, wogonin inhibited the expression of MMP-3, MMP-1, MMP-13, and ADAMTS-4, but increased expression of type II collagen. Furthermore, wogonin inhibited the production and proteolytic activity of MMP-3 in vitro, and inhibited production of MMP-3 protein in vivo. These results suggest that wogonin can regulate the gene expression and production of MMP-3, by directly acting on articular chondrocytes.

No MeSH data available.


Related in: MedlinePlus