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Characterization of Carriage Isolates of Neisseria meningitides in the Adolescents and Young Adults Population of Bogota (Colombia).

Moreno J, Hidalgo M, Duarte C, Sanabria O, Gabastou JM, Ibarz-Pavon AB - PLoS ONE (2015)

Bottom Line: Carriage was associated with cohabitation with smokers, and oral sex practices.Non-groupable and serogroup Y isolates were the most common capsule types found.Isolates presented a high genetic diversity, and circulation of the hypervirulent clonal complexes ST-23, ST-32 and ST-41/44 were detected.

View Article: PubMed Central - PubMed

Affiliation: Grupo de Microbiología, Instituto Nacional de Salud, Bogotá, Colombia.

ABSTRACT

Background: Meningococcal carriage studies are important to improve our understanding of the epidemiology of meningococcal disease. The aim of this study was to determine the prevalence of meningococcal carriage and the phenotypic and genotypic characteristics of isolates collected from a sample of students in the city of Bogotá, Colombia.

Materials and methods: A total of 1459 oropharyngeal samples were collected from students aged 15-21 years attending secondary schools and universities. Swabs were plated on a Thayer Martin agar and N. meningitidis was identified by standard microbiology methods and PCR.

Results: The overall carriage prevalence was 6.85%. Carriage was associated with cohabitation with smokers, and oral sex practices. Non-groupable and serogroup Y isolates were the most common capsule types found. Isolates presented a high genetic diversity, and circulation of the hypervirulent clonal complexes ST-23, ST-32 and ST-41/44 were detected.

Conclusion: The meningococcal carriage rate was lower than those reported in Europe and Africa, but higher than in other Latin American countries. Our data also revealed antigenic and genetic diversity of the isolates and the circulation of strains belonging to clonal complexes commonly associated with meningococcal disease.

No MeSH data available.


Related in: MedlinePlus

Distribution of serogroups according to N. meningitidis clonal complex.
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pone.0135497.g002: Distribution of serogroups according to N. meningitidis clonal complex.

Mentions: Sequence type was determined for 74 of the 100 meningococcal isolates. Overall, 72 isolates were grouped into one of 14 clonal complexes with different serotype:serosubtype combinations (Table 3). A total of 16 isolates (21.6%) belonged to the ST-23 clonal complex, of which 14 were group Y, and 12 presented the phenotype Y:14:NST. Most of the NG isolates were grouped into clonal complexes ST-53 (n = 13), ST-198 (n = 10), ST-1136 (n = 6) and ST-178 (n = 4). Group B isolates were distributed into the ST41/44, ST-32, ST-162 and ST-231 clonal complexes. Group W isolates belonged to the ST-22 complex, and two group C isolates, together with three NG were grouped into the ST-35 clonal complex (Fig 2).


Characterization of Carriage Isolates of Neisseria meningitides in the Adolescents and Young Adults Population of Bogota (Colombia).

Moreno J, Hidalgo M, Duarte C, Sanabria O, Gabastou JM, Ibarz-Pavon AB - PLoS ONE (2015)

Distribution of serogroups according to N. meningitidis clonal complex.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556189&req=5

pone.0135497.g002: Distribution of serogroups according to N. meningitidis clonal complex.
Mentions: Sequence type was determined for 74 of the 100 meningococcal isolates. Overall, 72 isolates were grouped into one of 14 clonal complexes with different serotype:serosubtype combinations (Table 3). A total of 16 isolates (21.6%) belonged to the ST-23 clonal complex, of which 14 were group Y, and 12 presented the phenotype Y:14:NST. Most of the NG isolates were grouped into clonal complexes ST-53 (n = 13), ST-198 (n = 10), ST-1136 (n = 6) and ST-178 (n = 4). Group B isolates were distributed into the ST41/44, ST-32, ST-162 and ST-231 clonal complexes. Group W isolates belonged to the ST-22 complex, and two group C isolates, together with three NG were grouped into the ST-35 clonal complex (Fig 2).

Bottom Line: Carriage was associated with cohabitation with smokers, and oral sex practices.Non-groupable and serogroup Y isolates were the most common capsule types found.Isolates presented a high genetic diversity, and circulation of the hypervirulent clonal complexes ST-23, ST-32 and ST-41/44 were detected.

View Article: PubMed Central - PubMed

Affiliation: Grupo de Microbiología, Instituto Nacional de Salud, Bogotá, Colombia.

ABSTRACT

Background: Meningococcal carriage studies are important to improve our understanding of the epidemiology of meningococcal disease. The aim of this study was to determine the prevalence of meningococcal carriage and the phenotypic and genotypic characteristics of isolates collected from a sample of students in the city of Bogotá, Colombia.

Materials and methods: A total of 1459 oropharyngeal samples were collected from students aged 15-21 years attending secondary schools and universities. Swabs were plated on a Thayer Martin agar and N. meningitidis was identified by standard microbiology methods and PCR.

Results: The overall carriage prevalence was 6.85%. Carriage was associated with cohabitation with smokers, and oral sex practices. Non-groupable and serogroup Y isolates were the most common capsule types found. Isolates presented a high genetic diversity, and circulation of the hypervirulent clonal complexes ST-23, ST-32 and ST-41/44 were detected.

Conclusion: The meningococcal carriage rate was lower than those reported in Europe and Africa, but higher than in other Latin American countries. Our data also revealed antigenic and genetic diversity of the isolates and the circulation of strains belonging to clonal complexes commonly associated with meningococcal disease.

No MeSH data available.


Related in: MedlinePlus