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Characterization of Carriage Isolates of Neisseria meningitides in the Adolescents and Young Adults Population of Bogota (Colombia).

Moreno J, Hidalgo M, Duarte C, Sanabria O, Gabastou JM, Ibarz-Pavon AB - PLoS ONE (2015)

Bottom Line: Carriage was associated with cohabitation with smokers, and oral sex practices.Non-groupable and serogroup Y isolates were the most common capsule types found.Isolates presented a high genetic diversity, and circulation of the hypervirulent clonal complexes ST-23, ST-32 and ST-41/44 were detected.

View Article: PubMed Central - PubMed

Affiliation: Grupo de Microbiología, Instituto Nacional de Salud, Bogotá, Colombia.

ABSTRACT

Background: Meningococcal carriage studies are important to improve our understanding of the epidemiology of meningococcal disease. The aim of this study was to determine the prevalence of meningococcal carriage and the phenotypic and genotypic characteristics of isolates collected from a sample of students in the city of Bogotá, Colombia.

Materials and methods: A total of 1459 oropharyngeal samples were collected from students aged 15-21 years attending secondary schools and universities. Swabs were plated on a Thayer Martin agar and N. meningitidis was identified by standard microbiology methods and PCR.

Results: The overall carriage prevalence was 6.85%. Carriage was associated with cohabitation with smokers, and oral sex practices. Non-groupable and serogroup Y isolates were the most common capsule types found. Isolates presented a high genetic diversity, and circulation of the hypervirulent clonal complexes ST-23, ST-32 and ST-41/44 were detected.

Conclusion: The meningococcal carriage rate was lower than those reported in Europe and Africa, but higher than in other Latin American countries. Our data also revealed antigenic and genetic diversity of the isolates and the circulation of strains belonging to clonal complexes commonly associated with meningococcal disease.

No MeSH data available.


Related in: MedlinePlus

Identification of serogroups of N. meningitidis with conventional slide agglutination and detection of capsule  (cnl) region.The figure represents the number of isolates of N. meningitidis by serogroup. Capsulated isolates for each serogroup are represed in black, whereas non-capsulated appear in white. For the NG, those presenting the cnl insertion appear in grey, and those that could not be serogrouped by either PCR or agglutination are represented in black.
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pone.0135497.g001: Identification of serogroups of N. meningitidis with conventional slide agglutination and detection of capsule (cnl) region.The figure represents the number of isolates of N. meningitidis by serogroup. Capsulated isolates for each serogroup are represed in black, whereas non-capsulated appear in white. For the NG, those presenting the cnl insertion appear in grey, and those that could not be serogrouped by either PCR or agglutination are represented in black.

Mentions: Using the PCR methodology, 32 isolates presented the cnl locus, and 25 were non-groupable. Group Y was identified in 22 isolates, of which 10 (45.5%) were expressing the capsule. A total of 15 isolates were group B, of which 7 (46.6%) were capsulated. All 3 serogroup C isolates were capsulated, and one of three W also expressed the capsule (Fig 1). A total of 31 different serotype:serosubtype combinations were found. The combination NT:NST was common among NG and groups C and W isolates. The combination 14:NST was mainly associated to group Y isolates. NG isolates were predominant among the combinations 15:P1.16 (7.0%) and NT:P1 (Table 3).


Characterization of Carriage Isolates of Neisseria meningitides in the Adolescents and Young Adults Population of Bogota (Colombia).

Moreno J, Hidalgo M, Duarte C, Sanabria O, Gabastou JM, Ibarz-Pavon AB - PLoS ONE (2015)

Identification of serogroups of N. meningitidis with conventional slide agglutination and detection of capsule  (cnl) region.The figure represents the number of isolates of N. meningitidis by serogroup. Capsulated isolates for each serogroup are represed in black, whereas non-capsulated appear in white. For the NG, those presenting the cnl insertion appear in grey, and those that could not be serogrouped by either PCR or agglutination are represented in black.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556189&req=5

pone.0135497.g001: Identification of serogroups of N. meningitidis with conventional slide agglutination and detection of capsule (cnl) region.The figure represents the number of isolates of N. meningitidis by serogroup. Capsulated isolates for each serogroup are represed in black, whereas non-capsulated appear in white. For the NG, those presenting the cnl insertion appear in grey, and those that could not be serogrouped by either PCR or agglutination are represented in black.
Mentions: Using the PCR methodology, 32 isolates presented the cnl locus, and 25 were non-groupable. Group Y was identified in 22 isolates, of which 10 (45.5%) were expressing the capsule. A total of 15 isolates were group B, of which 7 (46.6%) were capsulated. All 3 serogroup C isolates were capsulated, and one of three W also expressed the capsule (Fig 1). A total of 31 different serotype:serosubtype combinations were found. The combination NT:NST was common among NG and groups C and W isolates. The combination 14:NST was mainly associated to group Y isolates. NG isolates were predominant among the combinations 15:P1.16 (7.0%) and NT:P1 (Table 3).

Bottom Line: Carriage was associated with cohabitation with smokers, and oral sex practices.Non-groupable and serogroup Y isolates were the most common capsule types found.Isolates presented a high genetic diversity, and circulation of the hypervirulent clonal complexes ST-23, ST-32 and ST-41/44 were detected.

View Article: PubMed Central - PubMed

Affiliation: Grupo de Microbiología, Instituto Nacional de Salud, Bogotá, Colombia.

ABSTRACT

Background: Meningococcal carriage studies are important to improve our understanding of the epidemiology of meningococcal disease. The aim of this study was to determine the prevalence of meningococcal carriage and the phenotypic and genotypic characteristics of isolates collected from a sample of students in the city of Bogotá, Colombia.

Materials and methods: A total of 1459 oropharyngeal samples were collected from students aged 15-21 years attending secondary schools and universities. Swabs were plated on a Thayer Martin agar and N. meningitidis was identified by standard microbiology methods and PCR.

Results: The overall carriage prevalence was 6.85%. Carriage was associated with cohabitation with smokers, and oral sex practices. Non-groupable and serogroup Y isolates were the most common capsule types found. Isolates presented a high genetic diversity, and circulation of the hypervirulent clonal complexes ST-23, ST-32 and ST-41/44 were detected.

Conclusion: The meningococcal carriage rate was lower than those reported in Europe and Africa, but higher than in other Latin American countries. Our data also revealed antigenic and genetic diversity of the isolates and the circulation of strains belonging to clonal complexes commonly associated with meningococcal disease.

No MeSH data available.


Related in: MedlinePlus