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Rhizoma Paridis Saponins Induces Cell Cycle Arrest and Apoptosis in Non-Small Cell Lung Carcinoma A549 Cells.

Zhang J, Yang Y, Lei L, Tian M - Med. Sci. Monit. (2015)

Bottom Line: However, the molecular mechanism of the anticancer effect of RPS is unknown.In addition, the effect was dose-dependent.Moreover, the results of qRT-PCR and Western blotting showed that p53 and cyclin-dependent kinase 2 (CDK2) were significantly downregulated, and that BCL2, BAX, and p21 were upregulated, by RPS treatment.

View Article: PubMed Central - PubMed

Affiliation: School of Bioengineering, Chengdu University, Chengdu, Sichuan, China (mainland).

ABSTRACT

Background: As a traditional Chinese medicine herb, Chonglou (Paris polyphylla var. chensiins) has been used as anticancer medicine in China in recent decades, as it can induce cell cycle arrest and apoptosis in numerous cancer cells. The saponins extract from the rhizoma of Chonglou [Rhizoma Paridis saponins (RPS)] is known as the main active component for anticancer treatment. However, the molecular mechanism of the anticancer effect of RPS is unknown.

Material and methods: The present study evaluated the effect of RPS in non-small-cell lung cancer (NSCLC) A549 cells using the 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide (MTT) assay and flow cytometry. Subsequently, the expression of several genes associated with cell cycle and apoptosis were detected by reverse transcription-quantitative polymerase chain reaction (qRT-PCR) and Western blotting.

Results: RPS was revealed to inhibit cell growth, causing a number of cells to accumulate in the G 1 phase of the cell cycle, leading to apoptosis. In addition, the effect was dose-dependent. Moreover, the results of qRT-PCR and Western blotting showed that p53 and cyclin-dependent kinase 2 (CDK2) were significantly downregulated, and that BCL2, BAX, and p21 were upregulated, by RPS treatment.

Conclusions: We speculated that the RPS could act on a pathway, including p53, p21, BCL2, BAX, and CDK2, and results in G1 cell cycle arrest and apoptosis in NSCLC cells.

No MeSH data available.


Related in: MedlinePlus

Effect of Rhizoma Paridis saponins (RPS) on expression of cell cycle and apoptosis-related genes. Results of (A–K) quantitative polymerase chain reaction for genes and (L) Western blotting for the proteins. * P<0.05, as determined by a Student’s t-test compared with the control group.
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f3-medscimonit-21-2535: Effect of Rhizoma Paridis saponins (RPS) on expression of cell cycle and apoptosis-related genes. Results of (A–K) quantitative polymerase chain reaction for genes and (L) Western blotting for the proteins. * P<0.05, as determined by a Student’s t-test compared with the control group.

Mentions: As shown in Figure 3, the expression of p53, BCL2 and CDK2 were significantly downregulated by RPS treatment (Figure 3A, 3D, 3I). In comparison, the expression of p21 and BAX were significantly upregulated by RPS treatment (Figure 3B, 3C). However, the expression of cyclins A/B1/D1/E and CDK4/6 did not show any clear changes (Figure 3E–3H, 3J, 3K). Subsequently, Western blotting was used to detect the protein expression of p53, p21, BAX, BCL2, and CDK2, and the results verified that the expression of the 5 proteins changed in accordance with the qPCR results (Figure 3L).


Rhizoma Paridis Saponins Induces Cell Cycle Arrest and Apoptosis in Non-Small Cell Lung Carcinoma A549 Cells.

Zhang J, Yang Y, Lei L, Tian M - Med. Sci. Monit. (2015)

Effect of Rhizoma Paridis saponins (RPS) on expression of cell cycle and apoptosis-related genes. Results of (A–K) quantitative polymerase chain reaction for genes and (L) Western blotting for the proteins. * P<0.05, as determined by a Student’s t-test compared with the control group.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4556165&req=5

f3-medscimonit-21-2535: Effect of Rhizoma Paridis saponins (RPS) on expression of cell cycle and apoptosis-related genes. Results of (A–K) quantitative polymerase chain reaction for genes and (L) Western blotting for the proteins. * P<0.05, as determined by a Student’s t-test compared with the control group.
Mentions: As shown in Figure 3, the expression of p53, BCL2 and CDK2 were significantly downregulated by RPS treatment (Figure 3A, 3D, 3I). In comparison, the expression of p21 and BAX were significantly upregulated by RPS treatment (Figure 3B, 3C). However, the expression of cyclins A/B1/D1/E and CDK4/6 did not show any clear changes (Figure 3E–3H, 3J, 3K). Subsequently, Western blotting was used to detect the protein expression of p53, p21, BAX, BCL2, and CDK2, and the results verified that the expression of the 5 proteins changed in accordance with the qPCR results (Figure 3L).

Bottom Line: However, the molecular mechanism of the anticancer effect of RPS is unknown.In addition, the effect was dose-dependent.Moreover, the results of qRT-PCR and Western blotting showed that p53 and cyclin-dependent kinase 2 (CDK2) were significantly downregulated, and that BCL2, BAX, and p21 were upregulated, by RPS treatment.

View Article: PubMed Central - PubMed

Affiliation: School of Bioengineering, Chengdu University, Chengdu, Sichuan, China (mainland).

ABSTRACT

Background: As a traditional Chinese medicine herb, Chonglou (Paris polyphylla var. chensiins) has been used as anticancer medicine in China in recent decades, as it can induce cell cycle arrest and apoptosis in numerous cancer cells. The saponins extract from the rhizoma of Chonglou [Rhizoma Paridis saponins (RPS)] is known as the main active component for anticancer treatment. However, the molecular mechanism of the anticancer effect of RPS is unknown.

Material and methods: The present study evaluated the effect of RPS in non-small-cell lung cancer (NSCLC) A549 cells using the 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide (MTT) assay and flow cytometry. Subsequently, the expression of several genes associated with cell cycle and apoptosis were detected by reverse transcription-quantitative polymerase chain reaction (qRT-PCR) and Western blotting.

Results: RPS was revealed to inhibit cell growth, causing a number of cells to accumulate in the G 1 phase of the cell cycle, leading to apoptosis. In addition, the effect was dose-dependent. Moreover, the results of qRT-PCR and Western blotting showed that p53 and cyclin-dependent kinase 2 (CDK2) were significantly downregulated, and that BCL2, BAX, and p21 were upregulated, by RPS treatment.

Conclusions: We speculated that the RPS could act on a pathway, including p53, p21, BCL2, BAX, and CDK2, and results in G1 cell cycle arrest and apoptosis in NSCLC cells.

No MeSH data available.


Related in: MedlinePlus