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In Vitro Ion Chelating, Antioxidative Mechanism of Extracts from Fruits and Barks of Tetrapleura tetraptera and Their Protective Effects against Fenton Mediated Toxicity of Metal Ions on Liver Homogenates.

Moukette BM, Pieme AC, Biapa PC, Njimou JR, Stoller M, Bravi M, Yonkeu Ngogang J - Evid Based Complement Alternat Med (2015)

Bottom Line: The antioxidant activity of the extracts was investigated spectrophotometrically against several radicals (1,1-diphenyl-2-picrylhydrazyl (DPPH(•)), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS(•)), hydroxyl radical (HO(•)), and nitric oxide (NO(•))), followed by the ferric reducing power, total phenols, flavonoid, and flavonol contents.The results showed that the hydroethanolic extract of T. tetraptera (CFH) has the lowest IC50 value with the DPPH, ABTS, OH, and NO radicals.The same extract also exhibited the significantly higher level of total phenols (37.24 ± 2.00 CAE/g dried extract); flavonoids (11.36 ± 1.88 QE/g dried extract); and flavonols contents (3.95 ± 0.39 QE/g dried extract).

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biochemistry, Department of Biochemistry and Physiological Sciences, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, P.O. Box 1364, Yaounde, Cameroon.

ABSTRACT
The aim of the present study was to investigate the antioxidant activity and protective potential of T. tetraptera extracts against ion toxicity. The antioxidant activity of the extracts was investigated spectrophotometrically against several radicals (1,1-diphenyl-2-picrylhydrazyl (DPPH(•)), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS(•)), hydroxyl radical (HO(•)), and nitric oxide (NO(•))), followed by the ferric reducing power, total phenols, flavonoid, and flavonol contents. The effects of the extracts on catalase (CAT), superoxide dismutase (SOD), and peroxidase activities were also determined using the standard methods as well as the polyphenol profile using HPLC. The results showed that the hydroethanolic extract of T. tetraptera (CFH) has the lowest IC50 value with the DPPH, ABTS, OH, and NO radicals. The same extract also exhibited the significantly higher level of total phenols (37.24 ± 2.00 CAE/g dried extract); flavonoids (11.36 ± 1.88 QE/g dried extract); and flavonols contents (3.95 ± 0.39 QE/g dried extract). The HPLC profile of T. tetraptera revealed that eugenol (958.81 ± 00 mg/g DW), quercetin (353.78 ± 00 mg/g DW), and rutin (210.54 ± 00 mg/g DW) were higher in the fruit than the bark extracts. In conclusion, extracts from T. tetraptera may act as a protector against oxidative mediated ion toxicity.

No MeSH data available.


Related in: MedlinePlus

Principal component analysis results on F1 × F2 axis of in vitro antioxidant assays on rat liver enzymes for the tested extracts. Values are expressed as mean ± SD of three replicates. CEH: T. tetraptera hydroethanolic extract (bark); CFH: T. tetraptera hydroethanolic extract (fruit); CEE: T. tetraptera ethanolic extract (bark); CFE: T. tetraptera ethanolic extract (fruit); MOLYBDAT: phosphomolybdenum test; SOD: SOD activity test; catalase: catalase activity test; peroxidase: peroxidase activity test; flavonols: flavonol assay; polyphenol: polyphenol assay; flavonoids: flavonoid assay; FRAP: FRAP antioxidant test; INHIB MDA: MDA inhibition percentage.
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fig10: Principal component analysis results on F1 × F2 axis of in vitro antioxidant assays on rat liver enzymes for the tested extracts. Values are expressed as mean ± SD of three replicates. CEH: T. tetraptera hydroethanolic extract (bark); CFH: T. tetraptera hydroethanolic extract (fruit); CEE: T. tetraptera ethanolic extract (bark); CFE: T. tetraptera ethanolic extract (fruit); MOLYBDAT: phosphomolybdenum test; SOD: SOD activity test; catalase: catalase activity test; peroxidase: peroxidase activity test; flavonols: flavonol assay; polyphenol: polyphenol assay; flavonoids: flavonoid assay; FRAP: FRAP antioxidant test; INHIB MDA: MDA inhibition percentage.

Mentions: The correlation, principal component analysis of the antioxidant assays of the different extracts are presented in Figures 9 and 10 and Tables 4 and 5. The different samples and the antioxidant tests have been projected in a single system; according to the ACP analysis, 93.08% of the variation exists in this system with a contribution of 88.02% for the axis F1 and 5.06 for the axis F2.


In Vitro Ion Chelating, Antioxidative Mechanism of Extracts from Fruits and Barks of Tetrapleura tetraptera and Their Protective Effects against Fenton Mediated Toxicity of Metal Ions on Liver Homogenates.

Moukette BM, Pieme AC, Biapa PC, Njimou JR, Stoller M, Bravi M, Yonkeu Ngogang J - Evid Based Complement Alternat Med (2015)

Principal component analysis results on F1 × F2 axis of in vitro antioxidant assays on rat liver enzymes for the tested extracts. Values are expressed as mean ± SD of three replicates. CEH: T. tetraptera hydroethanolic extract (bark); CFH: T. tetraptera hydroethanolic extract (fruit); CEE: T. tetraptera ethanolic extract (bark); CFE: T. tetraptera ethanolic extract (fruit); MOLYBDAT: phosphomolybdenum test; SOD: SOD activity test; catalase: catalase activity test; peroxidase: peroxidase activity test; flavonols: flavonol assay; polyphenol: polyphenol assay; flavonoids: flavonoid assay; FRAP: FRAP antioxidant test; INHIB MDA: MDA inhibition percentage.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4556075&req=5

fig10: Principal component analysis results on F1 × F2 axis of in vitro antioxidant assays on rat liver enzymes for the tested extracts. Values are expressed as mean ± SD of three replicates. CEH: T. tetraptera hydroethanolic extract (bark); CFH: T. tetraptera hydroethanolic extract (fruit); CEE: T. tetraptera ethanolic extract (bark); CFE: T. tetraptera ethanolic extract (fruit); MOLYBDAT: phosphomolybdenum test; SOD: SOD activity test; catalase: catalase activity test; peroxidase: peroxidase activity test; flavonols: flavonol assay; polyphenol: polyphenol assay; flavonoids: flavonoid assay; FRAP: FRAP antioxidant test; INHIB MDA: MDA inhibition percentage.
Mentions: The correlation, principal component analysis of the antioxidant assays of the different extracts are presented in Figures 9 and 10 and Tables 4 and 5. The different samples and the antioxidant tests have been projected in a single system; according to the ACP analysis, 93.08% of the variation exists in this system with a contribution of 88.02% for the axis F1 and 5.06 for the axis F2.

Bottom Line: The antioxidant activity of the extracts was investigated spectrophotometrically against several radicals (1,1-diphenyl-2-picrylhydrazyl (DPPH(•)), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS(•)), hydroxyl radical (HO(•)), and nitric oxide (NO(•))), followed by the ferric reducing power, total phenols, flavonoid, and flavonol contents.The results showed that the hydroethanolic extract of T. tetraptera (CFH) has the lowest IC50 value with the DPPH, ABTS, OH, and NO radicals.The same extract also exhibited the significantly higher level of total phenols (37.24 ± 2.00 CAE/g dried extract); flavonoids (11.36 ± 1.88 QE/g dried extract); and flavonols contents (3.95 ± 0.39 QE/g dried extract).

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biochemistry, Department of Biochemistry and Physiological Sciences, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, P.O. Box 1364, Yaounde, Cameroon.

ABSTRACT
The aim of the present study was to investigate the antioxidant activity and protective potential of T. tetraptera extracts against ion toxicity. The antioxidant activity of the extracts was investigated spectrophotometrically against several radicals (1,1-diphenyl-2-picrylhydrazyl (DPPH(•)), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS(•)), hydroxyl radical (HO(•)), and nitric oxide (NO(•))), followed by the ferric reducing power, total phenols, flavonoid, and flavonol contents. The effects of the extracts on catalase (CAT), superoxide dismutase (SOD), and peroxidase activities were also determined using the standard methods as well as the polyphenol profile using HPLC. The results showed that the hydroethanolic extract of T. tetraptera (CFH) has the lowest IC50 value with the DPPH, ABTS, OH, and NO radicals. The same extract also exhibited the significantly higher level of total phenols (37.24 ± 2.00 CAE/g dried extract); flavonoids (11.36 ± 1.88 QE/g dried extract); and flavonols contents (3.95 ± 0.39 QE/g dried extract). The HPLC profile of T. tetraptera revealed that eugenol (958.81 ± 00 mg/g DW), quercetin (353.78 ± 00 mg/g DW), and rutin (210.54 ± 00 mg/g DW) were higher in the fruit than the bark extracts. In conclusion, extracts from T. tetraptera may act as a protector against oxidative mediated ion toxicity.

No MeSH data available.


Related in: MedlinePlus