Limits...
In Vitro Ion Chelating, Antioxidative Mechanism of Extracts from Fruits and Barks of Tetrapleura tetraptera and Their Protective Effects against Fenton Mediated Toxicity of Metal Ions on Liver Homogenates.

Moukette BM, Pieme AC, Biapa PC, Njimou JR, Stoller M, Bravi M, Yonkeu Ngogang J - Evid Based Complement Alternat Med (2015)

Bottom Line: The antioxidant activity of the extracts was investigated spectrophotometrically against several radicals (1,1-diphenyl-2-picrylhydrazyl (DPPH(•)), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS(•)), hydroxyl radical (HO(•)), and nitric oxide (NO(•))), followed by the ferric reducing power, total phenols, flavonoid, and flavonol contents.The results showed that the hydroethanolic extract of T. tetraptera (CFH) has the lowest IC50 value with the DPPH, ABTS, OH, and NO radicals.The same extract also exhibited the significantly higher level of total phenols (37.24 ± 2.00 CAE/g dried extract); flavonoids (11.36 ± 1.88 QE/g dried extract); and flavonols contents (3.95 ± 0.39 QE/g dried extract).

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biochemistry, Department of Biochemistry and Physiological Sciences, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, P.O. Box 1364, Yaounde, Cameroon.

ABSTRACT
The aim of the present study was to investigate the antioxidant activity and protective potential of T. tetraptera extracts against ion toxicity. The antioxidant activity of the extracts was investigated spectrophotometrically against several radicals (1,1-diphenyl-2-picrylhydrazyl (DPPH(•)), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS(•)), hydroxyl radical (HO(•)), and nitric oxide (NO(•))), followed by the ferric reducing power, total phenols, flavonoid, and flavonol contents. The effects of the extracts on catalase (CAT), superoxide dismutase (SOD), and peroxidase activities were also determined using the standard methods as well as the polyphenol profile using HPLC. The results showed that the hydroethanolic extract of T. tetraptera (CFH) has the lowest IC50 value with the DPPH, ABTS, OH, and NO radicals. The same extract also exhibited the significantly higher level of total phenols (37.24 ± 2.00 CAE/g dried extract); flavonoids (11.36 ± 1.88 QE/g dried extract); and flavonols contents (3.95 ± 0.39 QE/g dried extract). The HPLC profile of T. tetraptera revealed that eugenol (958.81 ± 00 mg/g DW), quercetin (353.78 ± 00 mg/g DW), and rutin (210.54 ± 00 mg/g DW) were higher in the fruit than the bark extracts. In conclusion, extracts from T. tetraptera may act as a protector against oxidative mediated ion toxicity.

No MeSH data available.


Related in: MedlinePlus

HPLC chromatograms of phenolic extracts from T. tetraptera recorded at 280 nm; (a) barks; (b) fruits (TR: 19.10: 3,4-OH benzoic acid; 33.49: apigenin; 25.67: caffeic acid; 23.48: catechin; 29.43: eugenol; 14.38; gallic acid; 25.11: O-coumaric; 21.91: OH-tyrosol; 30.52: P-coumaric acid. 42.19: quercetin; 29.45: rutin; 25.55: syringic acid; 17.35: theobromine; 21.77: tyrosol and 25.27: vanillic acid).
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4556075&req=5

fig1: HPLC chromatograms of phenolic extracts from T. tetraptera recorded at 280 nm; (a) barks; (b) fruits (TR: 19.10: 3,4-OH benzoic acid; 33.49: apigenin; 25.67: caffeic acid; 23.48: catechin; 29.43: eugenol; 14.38; gallic acid; 25.11: O-coumaric; 21.91: OH-tyrosol; 30.52: P-coumaric acid. 42.19: quercetin; 29.45: rutin; 25.55: syringic acid; 17.35: theobromine; 21.77: tyrosol and 25.27: vanillic acid).

Mentions: The HPLC profile of the polyphenols from bark and fruit extracts of T. tetraptera is represented in Figures 1(a) and 1(b) and Table 2. These results demonstrated that polyphenols molecules are more concentrated in the fruits than the barks. The most represented phenolic compounds such as eugenol, quercetin, rutin, and tyrosol are 5.88–10.79-fold more concentrated in the fruits than the barks (Table 2).


In Vitro Ion Chelating, Antioxidative Mechanism of Extracts from Fruits and Barks of Tetrapleura tetraptera and Their Protective Effects against Fenton Mediated Toxicity of Metal Ions on Liver Homogenates.

Moukette BM, Pieme AC, Biapa PC, Njimou JR, Stoller M, Bravi M, Yonkeu Ngogang J - Evid Based Complement Alternat Med (2015)

HPLC chromatograms of phenolic extracts from T. tetraptera recorded at 280 nm; (a) barks; (b) fruits (TR: 19.10: 3,4-OH benzoic acid; 33.49: apigenin; 25.67: caffeic acid; 23.48: catechin; 29.43: eugenol; 14.38; gallic acid; 25.11: O-coumaric; 21.91: OH-tyrosol; 30.52: P-coumaric acid. 42.19: quercetin; 29.45: rutin; 25.55: syringic acid; 17.35: theobromine; 21.77: tyrosol and 25.27: vanillic acid).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4556075&req=5

fig1: HPLC chromatograms of phenolic extracts from T. tetraptera recorded at 280 nm; (a) barks; (b) fruits (TR: 19.10: 3,4-OH benzoic acid; 33.49: apigenin; 25.67: caffeic acid; 23.48: catechin; 29.43: eugenol; 14.38; gallic acid; 25.11: O-coumaric; 21.91: OH-tyrosol; 30.52: P-coumaric acid. 42.19: quercetin; 29.45: rutin; 25.55: syringic acid; 17.35: theobromine; 21.77: tyrosol and 25.27: vanillic acid).
Mentions: The HPLC profile of the polyphenols from bark and fruit extracts of T. tetraptera is represented in Figures 1(a) and 1(b) and Table 2. These results demonstrated that polyphenols molecules are more concentrated in the fruits than the barks. The most represented phenolic compounds such as eugenol, quercetin, rutin, and tyrosol are 5.88–10.79-fold more concentrated in the fruits than the barks (Table 2).

Bottom Line: The antioxidant activity of the extracts was investigated spectrophotometrically against several radicals (1,1-diphenyl-2-picrylhydrazyl (DPPH(•)), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS(•)), hydroxyl radical (HO(•)), and nitric oxide (NO(•))), followed by the ferric reducing power, total phenols, flavonoid, and flavonol contents.The results showed that the hydroethanolic extract of T. tetraptera (CFH) has the lowest IC50 value with the DPPH, ABTS, OH, and NO radicals.The same extract also exhibited the significantly higher level of total phenols (37.24 ± 2.00 CAE/g dried extract); flavonoids (11.36 ± 1.88 QE/g dried extract); and flavonols contents (3.95 ± 0.39 QE/g dried extract).

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biochemistry, Department of Biochemistry and Physiological Sciences, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, P.O. Box 1364, Yaounde, Cameroon.

ABSTRACT
The aim of the present study was to investigate the antioxidant activity and protective potential of T. tetraptera extracts against ion toxicity. The antioxidant activity of the extracts was investigated spectrophotometrically against several radicals (1,1-diphenyl-2-picrylhydrazyl (DPPH(•)), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS(•)), hydroxyl radical (HO(•)), and nitric oxide (NO(•))), followed by the ferric reducing power, total phenols, flavonoid, and flavonol contents. The effects of the extracts on catalase (CAT), superoxide dismutase (SOD), and peroxidase activities were also determined using the standard methods as well as the polyphenol profile using HPLC. The results showed that the hydroethanolic extract of T. tetraptera (CFH) has the lowest IC50 value with the DPPH, ABTS, OH, and NO radicals. The same extract also exhibited the significantly higher level of total phenols (37.24 ± 2.00 CAE/g dried extract); flavonoids (11.36 ± 1.88 QE/g dried extract); and flavonols contents (3.95 ± 0.39 QE/g dried extract). The HPLC profile of T. tetraptera revealed that eugenol (958.81 ± 00 mg/g DW), quercetin (353.78 ± 00 mg/g DW), and rutin (210.54 ± 00 mg/g DW) were higher in the fruit than the bark extracts. In conclusion, extracts from T. tetraptera may act as a protector against oxidative mediated ion toxicity.

No MeSH data available.


Related in: MedlinePlus