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Robust and high resolution hyperpolarized metabolic imaging of the rat heart at 7 T with 3D spectral-spatial EPI.

Miller JJ, Lau AZ, Teh I, Schneider JE, Kinchesh P, Smart S, Ball V, Sibson NR, Tyler DJ - Magn Reson Med (2015)

Bottom Line: Significant differences in myocardial pyruvate dehydrogenase flux were observed between the three groups of animals, concomitant with the known biochemistry.The field of view enabled the simultaneous multi-organ acquisition of metabolic information from the rat, which is of great utility for preclinical research in cardiovascular disease.Magnetic Resonance in Medicine published by Wiley Periodicals, Inc. on behalf of International Society for Magnetic Resonance.

View Article: PubMed Central - PubMed

Affiliation: Department of Physics, Clarendon Laboratory, University of Oxford, England, UK.

No MeSH data available.


Related in: MedlinePlus

The ratio of maximum bicarbonate/pyruvate was significantly different across the three groups of animals scanned, reflecting the known inhibition of PDK by DCA. (* denotes P < 0.05; ** P < 10−5.)
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Figure 7: The ratio of maximum bicarbonate/pyruvate was significantly different across the three groups of animals scanned, reflecting the known inhibition of PDK by DCA. (* denotes P < 0.05; ** P < 10−5.)

Mentions: Reflecting the known modulation of PDK by both DCA and fasting, significant differences in the ratio of the summed myocardial maximum bicarbonate to maximum pyruvate were observed across the three groups of animals scanned (Fig. 7; Student’s t-test P = 0.03 between fasted and fed, and fed and DCA; P = 0.0008 between fasted and DCA). The administration of DCA serves to approximately double this ratio compared to the fed animals, and quadruple it compared to fasted animals (DCA to fed: 1.6 ± 0.3, DCA to fasted: 3.9 ± 2.8; values ratio means ± S.E.M. propagated in quadrature). This doubling of PDH flux between fed animals and those dosed with DCA is the same as that obtained when this experiment was performed by simple unlocalized 13C spectroscopy following hyperpolarized [1-13C]pyruvate injection (18).


Robust and high resolution hyperpolarized metabolic imaging of the rat heart at 7 T with 3D spectral-spatial EPI.

Miller JJ, Lau AZ, Teh I, Schneider JE, Kinchesh P, Smart S, Ball V, Sibson NR, Tyler DJ - Magn Reson Med (2015)

The ratio of maximum bicarbonate/pyruvate was significantly different across the three groups of animals scanned, reflecting the known inhibition of PDK by DCA. (* denotes P < 0.05; ** P < 10−5.)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556070&req=5

Figure 7: The ratio of maximum bicarbonate/pyruvate was significantly different across the three groups of animals scanned, reflecting the known inhibition of PDK by DCA. (* denotes P < 0.05; ** P < 10−5.)
Mentions: Reflecting the known modulation of PDK by both DCA and fasting, significant differences in the ratio of the summed myocardial maximum bicarbonate to maximum pyruvate were observed across the three groups of animals scanned (Fig. 7; Student’s t-test P = 0.03 between fasted and fed, and fed and DCA; P = 0.0008 between fasted and DCA). The administration of DCA serves to approximately double this ratio compared to the fed animals, and quadruple it compared to fasted animals (DCA to fed: 1.6 ± 0.3, DCA to fasted: 3.9 ± 2.8; values ratio means ± S.E.M. propagated in quadrature). This doubling of PDH flux between fed animals and those dosed with DCA is the same as that obtained when this experiment was performed by simple unlocalized 13C spectroscopy following hyperpolarized [1-13C]pyruvate injection (18).

Bottom Line: Significant differences in myocardial pyruvate dehydrogenase flux were observed between the three groups of animals, concomitant with the known biochemistry.The field of view enabled the simultaneous multi-organ acquisition of metabolic information from the rat, which is of great utility for preclinical research in cardiovascular disease.Magnetic Resonance in Medicine published by Wiley Periodicals, Inc. on behalf of International Society for Magnetic Resonance.

View Article: PubMed Central - PubMed

Affiliation: Department of Physics, Clarendon Laboratory, University of Oxford, England, UK.

No MeSH data available.


Related in: MedlinePlus