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Robust and high resolution hyperpolarized metabolic imaging of the rat heart at 7 T with 3D spectral-spatial EPI.

Miller JJ, Lau AZ, Teh I, Schneider JE, Kinchesh P, Smart S, Ball V, Sibson NR, Tyler DJ - Magn Reson Med (2015)

Bottom Line: Significant differences in myocardial pyruvate dehydrogenase flux were observed between the three groups of animals, concomitant with the known biochemistry.The field of view enabled the simultaneous multi-organ acquisition of metabolic information from the rat, which is of great utility for preclinical research in cardiovascular disease.Magnetic Resonance in Medicine published by Wiley Periodicals, Inc. on behalf of International Society for Magnetic Resonance.

View Article: PubMed Central - PubMed

Affiliation: Department of Physics, Clarendon Laboratory, University of Oxford, England, UK.

No MeSH data available.


Related in: MedlinePlus

Representative axial maximum intensity projections of data acquired from animals fed and dosed with DCA. Images shown concatenated with z decreasing from left to right; the reference cine images (top) span the neck (top left), heart (top middle), and anterior lobes of the liver (top right). Pyruvate was resolved in the ventricles of the heart, and additionally in the inferior vena cava. The production of hyperpolarized bicarbonate occurred predominantly in the myocardium, and in this instance it was not possible to resolve metabolism in segments distal from the chest wall. Hyperpolarized [1-13C] lactate was also detected after production in the myocardium, and, to a far lesser extent, the liver.
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Figure 4: Representative axial maximum intensity projections of data acquired from animals fed and dosed with DCA. Images shown concatenated with z decreasing from left to right; the reference cine images (top) span the neck (top left), heart (top middle), and anterior lobes of the liver (top right). Pyruvate was resolved in the ventricles of the heart, and additionally in the inferior vena cava. The production of hyperpolarized bicarbonate occurred predominantly in the myocardium, and in this instance it was not possible to resolve metabolism in segments distal from the chest wall. Hyperpolarized [1-13C] lactate was also detected after production in the myocardium, and, to a far lesser extent, the liver.

Mentions: We were able to resolve the metabolism of prepolarized [1-13C]pyruvate in the in vivo rat heart with a reconstructed spatiotemporal resolution of 1 × 1 × 1.9 mm3 and ~1.8 s. The three-dimensional sequence reports on hyperpolarized metabolism in a 64 × 64 × 45.5 mm3 FOV, which was sufficient to encompass the whole heart and the liver. The large field of view was advantageous as we obtained information from multiple organs at the same point in time. The data were five-dimensional and an example maximum intensity projection (summed over time) showing the acquisition volume is illustrated in Figure 4.


Robust and high resolution hyperpolarized metabolic imaging of the rat heart at 7 T with 3D spectral-spatial EPI.

Miller JJ, Lau AZ, Teh I, Schneider JE, Kinchesh P, Smart S, Ball V, Sibson NR, Tyler DJ - Magn Reson Med (2015)

Representative axial maximum intensity projections of data acquired from animals fed and dosed with DCA. Images shown concatenated with z decreasing from left to right; the reference cine images (top) span the neck (top left), heart (top middle), and anterior lobes of the liver (top right). Pyruvate was resolved in the ventricles of the heart, and additionally in the inferior vena cava. The production of hyperpolarized bicarbonate occurred predominantly in the myocardium, and in this instance it was not possible to resolve metabolism in segments distal from the chest wall. Hyperpolarized [1-13C] lactate was also detected after production in the myocardium, and, to a far lesser extent, the liver.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556070&req=5

Figure 4: Representative axial maximum intensity projections of data acquired from animals fed and dosed with DCA. Images shown concatenated with z decreasing from left to right; the reference cine images (top) span the neck (top left), heart (top middle), and anterior lobes of the liver (top right). Pyruvate was resolved in the ventricles of the heart, and additionally in the inferior vena cava. The production of hyperpolarized bicarbonate occurred predominantly in the myocardium, and in this instance it was not possible to resolve metabolism in segments distal from the chest wall. Hyperpolarized [1-13C] lactate was also detected after production in the myocardium, and, to a far lesser extent, the liver.
Mentions: We were able to resolve the metabolism of prepolarized [1-13C]pyruvate in the in vivo rat heart with a reconstructed spatiotemporal resolution of 1 × 1 × 1.9 mm3 and ~1.8 s. The three-dimensional sequence reports on hyperpolarized metabolism in a 64 × 64 × 45.5 mm3 FOV, which was sufficient to encompass the whole heart and the liver. The large field of view was advantageous as we obtained information from multiple organs at the same point in time. The data were five-dimensional and an example maximum intensity projection (summed over time) showing the acquisition volume is illustrated in Figure 4.

Bottom Line: Significant differences in myocardial pyruvate dehydrogenase flux were observed between the three groups of animals, concomitant with the known biochemistry.The field of view enabled the simultaneous multi-organ acquisition of metabolic information from the rat, which is of great utility for preclinical research in cardiovascular disease.Magnetic Resonance in Medicine published by Wiley Periodicals, Inc. on behalf of International Society for Magnetic Resonance.

View Article: PubMed Central - PubMed

Affiliation: Department of Physics, Clarendon Laboratory, University of Oxford, England, UK.

No MeSH data available.


Related in: MedlinePlus