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Phytochemical capacity of Nitraria retusa leaves extracts inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma.

Boubaker J, Mokdad Bzeouich I, Nasr N, Ben Ghozlen H, Mustapha N, Ghedira K, Chekir-Ghedira L - BMC Complement Altern Med (2015)

Bottom Line: Amounts of melanin and tyrosinase were measured spectrophotometrically at 475 nm.Extracts from Nitraria retusa exhibited significant anti-proliferative activity after 48 h of incubation.The tested extracts have a significant biological effect which may be due to their bioactive compounds.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Rue Avicenne, Monastir, 5000, Tunisia. jihed.boubaker@yahoo.fr.

ABSTRACT

Background: Here, phytochemical profile of Nitraria retusa (N. Retusa) leaf extracts was identified and their ability to induce apoptosis and inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma was evaluated.

Methods: The Apoptosis was evidenced by investigating DNA fragmentation, and Acridine orange/ethidium bromide staining. Amounts of melanin and tyrosinase were measured spectrophotometrically at 475 nm.

Results: Extracts from Nitraria retusa exhibited significant anti-proliferative activity after 48 h of incubation. Our result was confirmed by ladder DNA fragmentation profile. All extracts showed also the ability to enhance melanogenesis and tyrosinase activity of B16F10 melanoma cells.

Conclusion: The tested extracts have a significant biological effect which may be due to their bioactive compounds.

No MeSH data available.


Related in: MedlinePlus

Effect of Nitraria retusa extracts on tyrosinase activity in B16-F10 cells after 48 h incubation. Values represent the means ± SD of three separate experiments. The statistical significance of results was evaluated by the Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001 means significant difference between control and treated sample
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Fig3: Effect of Nitraria retusa extracts on tyrosinase activity in B16-F10 cells after 48 h incubation. Values represent the means ± SD of three separate experiments. The statistical significance of results was evaluated by the Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001 means significant difference between control and treated sample

Mentions: Likewise, tyrosinase activity in B16-F10 cells treated with different extracts increases in a time-dependent manner (Fig. 3).Fig. 3


Phytochemical capacity of Nitraria retusa leaves extracts inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma.

Boubaker J, Mokdad Bzeouich I, Nasr N, Ben Ghozlen H, Mustapha N, Ghedira K, Chekir-Ghedira L - BMC Complement Altern Med (2015)

Effect of Nitraria retusa extracts on tyrosinase activity in B16-F10 cells after 48 h incubation. Values represent the means ± SD of three separate experiments. The statistical significance of results was evaluated by the Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001 means significant difference between control and treated sample
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4556050&req=5

Fig3: Effect of Nitraria retusa extracts on tyrosinase activity in B16-F10 cells after 48 h incubation. Values represent the means ± SD of three separate experiments. The statistical significance of results was evaluated by the Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001 means significant difference between control and treated sample
Mentions: Likewise, tyrosinase activity in B16-F10 cells treated with different extracts increases in a time-dependent manner (Fig. 3).Fig. 3

Bottom Line: Amounts of melanin and tyrosinase were measured spectrophotometrically at 475 nm.Extracts from Nitraria retusa exhibited significant anti-proliferative activity after 48 h of incubation.The tested extracts have a significant biological effect which may be due to their bioactive compounds.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Rue Avicenne, Monastir, 5000, Tunisia. jihed.boubaker@yahoo.fr.

ABSTRACT

Background: Here, phytochemical profile of Nitraria retusa (N. Retusa) leaf extracts was identified and their ability to induce apoptosis and inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma was evaluated.

Methods: The Apoptosis was evidenced by investigating DNA fragmentation, and Acridine orange/ethidium bromide staining. Amounts of melanin and tyrosinase were measured spectrophotometrically at 475 nm.

Results: Extracts from Nitraria retusa exhibited significant anti-proliferative activity after 48 h of incubation. Our result was confirmed by ladder DNA fragmentation profile. All extracts showed also the ability to enhance melanogenesis and tyrosinase activity of B16F10 melanoma cells.

Conclusion: The tested extracts have a significant biological effect which may be due to their bioactive compounds.

No MeSH data available.


Related in: MedlinePlus