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Phytochemical capacity of Nitraria retusa leaves extracts inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma.

Boubaker J, Mokdad Bzeouich I, Nasr N, Ben Ghozlen H, Mustapha N, Ghedira K, Chekir-Ghedira L - BMC Complement Altern Med (2015)

Bottom Line: Amounts of melanin and tyrosinase were measured spectrophotometrically at 475 nm.Extracts from Nitraria retusa exhibited significant anti-proliferative activity after 48 h of incubation.The tested extracts have a significant biological effect which may be due to their bioactive compounds.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Rue Avicenne, Monastir, 5000, Tunisia. jihed.boubaker@yahoo.fr.

ABSTRACT

Background: Here, phytochemical profile of Nitraria retusa (N. Retusa) leaf extracts was identified and their ability to induce apoptosis and inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma was evaluated.

Methods: The Apoptosis was evidenced by investigating DNA fragmentation, and Acridine orange/ethidium bromide staining. Amounts of melanin and tyrosinase were measured spectrophotometrically at 475 nm.

Results: Extracts from Nitraria retusa exhibited significant anti-proliferative activity after 48 h of incubation. Our result was confirmed by ladder DNA fragmentation profile. All extracts showed also the ability to enhance melanogenesis and tyrosinase activity of B16F10 melanoma cells.

Conclusion: The tested extracts have a significant biological effect which may be due to their bioactive compounds.

No MeSH data available.


Related in: MedlinePlus

DNA electrophoretic profiles of B16-F10 cells treated with different concentrations of Hex (hexane), Chl (chloroform), EA (ethyl acetate) and MeOH (methanol) extracts during 48 h h. DNA was separated on 1.5 % agarose gel. a PC: B16-F10 cell DNA; DNA of cells treated with b Hex 340 μg/assay Hex extract, c 170 μg/assay Hex extract, d 85 μg/assay Hex extract, e 80 μg/assay Chl extract, f 40 μg/assay Chl extract, g 20 μg/assay Chl extract, h 50 μg/assay EA extract, i 25 μg/assay EA extract, j 12.5 μg/assay EA extract, k 1000 μg/assay MeOH extract, l 500 μg/assay MeOH extract, m 250 μg/assay MeOH extract
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Fig2: DNA electrophoretic profiles of B16-F10 cells treated with different concentrations of Hex (hexane), Chl (chloroform), EA (ethyl acetate) and MeOH (methanol) extracts during 48 h h. DNA was separated on 1.5 % agarose gel. a PC: B16-F10 cell DNA; DNA of cells treated with b Hex 340 μg/assay Hex extract, c 170 μg/assay Hex extract, d 85 μg/assay Hex extract, e 80 μg/assay Chl extract, f 40 μg/assay Chl extract, g 20 μg/assay Chl extract, h 50 μg/assay EA extract, i 25 μg/assay EA extract, j 12.5 μg/assay EA extract, k 1000 μg/assay MeOH extract, l 500 μg/assay MeOH extract, m 250 μg/assay MeOH extract

Mentions: At exposure with different concentrations of Hex extract (Fig. 2, tracks b, c, d), Chl extract (Fig. 2, tracks e, f, g), EA extract (Fig. 2, tracks h, i, j) and MeOH extract (K,L,M) during 48 h, a fragmented DNA profile was clearly observed in B16-F10 cells treated with Chl and EA extracts, compared to untreated cells which did not provide a ladder DNA profile (Fig. 2, track a).Fig. 2


Phytochemical capacity of Nitraria retusa leaves extracts inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma.

Boubaker J, Mokdad Bzeouich I, Nasr N, Ben Ghozlen H, Mustapha N, Ghedira K, Chekir-Ghedira L - BMC Complement Altern Med (2015)

DNA electrophoretic profiles of B16-F10 cells treated with different concentrations of Hex (hexane), Chl (chloroform), EA (ethyl acetate) and MeOH (methanol) extracts during 48 h h. DNA was separated on 1.5 % agarose gel. a PC: B16-F10 cell DNA; DNA of cells treated with b Hex 340 μg/assay Hex extract, c 170 μg/assay Hex extract, d 85 μg/assay Hex extract, e 80 μg/assay Chl extract, f 40 μg/assay Chl extract, g 20 μg/assay Chl extract, h 50 μg/assay EA extract, i 25 μg/assay EA extract, j 12.5 μg/assay EA extract, k 1000 μg/assay MeOH extract, l 500 μg/assay MeOH extract, m 250 μg/assay MeOH extract
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4556050&req=5

Fig2: DNA electrophoretic profiles of B16-F10 cells treated with different concentrations of Hex (hexane), Chl (chloroform), EA (ethyl acetate) and MeOH (methanol) extracts during 48 h h. DNA was separated on 1.5 % agarose gel. a PC: B16-F10 cell DNA; DNA of cells treated with b Hex 340 μg/assay Hex extract, c 170 μg/assay Hex extract, d 85 μg/assay Hex extract, e 80 μg/assay Chl extract, f 40 μg/assay Chl extract, g 20 μg/assay Chl extract, h 50 μg/assay EA extract, i 25 μg/assay EA extract, j 12.5 μg/assay EA extract, k 1000 μg/assay MeOH extract, l 500 μg/assay MeOH extract, m 250 μg/assay MeOH extract
Mentions: At exposure with different concentrations of Hex extract (Fig. 2, tracks b, c, d), Chl extract (Fig. 2, tracks e, f, g), EA extract (Fig. 2, tracks h, i, j) and MeOH extract (K,L,M) during 48 h, a fragmented DNA profile was clearly observed in B16-F10 cells treated with Chl and EA extracts, compared to untreated cells which did not provide a ladder DNA profile (Fig. 2, track a).Fig. 2

Bottom Line: Amounts of melanin and tyrosinase were measured spectrophotometrically at 475 nm.Extracts from Nitraria retusa exhibited significant anti-proliferative activity after 48 h of incubation.The tested extracts have a significant biological effect which may be due to their bioactive compounds.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Rue Avicenne, Monastir, 5000, Tunisia. jihed.boubaker@yahoo.fr.

ABSTRACT

Background: Here, phytochemical profile of Nitraria retusa (N. Retusa) leaf extracts was identified and their ability to induce apoptosis and inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma was evaluated.

Methods: The Apoptosis was evidenced by investigating DNA fragmentation, and Acridine orange/ethidium bromide staining. Amounts of melanin and tyrosinase were measured spectrophotometrically at 475 nm.

Results: Extracts from Nitraria retusa exhibited significant anti-proliferative activity after 48 h of incubation. Our result was confirmed by ladder DNA fragmentation profile. All extracts showed also the ability to enhance melanogenesis and tyrosinase activity of B16F10 melanoma cells.

Conclusion: The tested extracts have a significant biological effect which may be due to their bioactive compounds.

No MeSH data available.


Related in: MedlinePlus