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Effects of magnetic nanoparticles of Fe3O4 combinated with gambogic acid on apoptosis of SMMC-7721 cells.

Tian L, Chen BA, Cheng J, Guo QL - Onco Targets Ther (2015)

Bottom Line: This study aims to investigate the potential benefit of combination therapy with magnetic nanoparticles of Fe3O4 (Fe3O4-MNP) and gambogic acid (GA) on SMMC-7721 cells.The Fe3O4-MNP itself had no obviously inhibitory effect, but it could enhance the effect of GA on proliferation of SMMC-7721 cells.Fe3O4-MNP can promote GA-induced apoptosis of SMMC-7721 cells, which may be related to the downregulation of Bcl-2 and upregulation of caspase-3.

View Article: PubMed Central - PubMed

Affiliation: Department of Hematology and Oncology (Key Department of Jiangsu Medicine), The Affiliated Zhongda Hospital, Medical School, Southeast University, Nanjing, Jiangsu, People's Republic of China.

ABSTRACT

Objective: This study aims to investigate the potential benefit of combination therapy with magnetic nanoparticles of Fe3O4 (Fe3O4-MNP) and gambogic acid (GA) on SMMC-7721 cells.

Methods: The inhibition of proliferation of SMMC-7721 cells was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Cell apoptosis was calculated and analyzed by flow cytometry, and the expressions of the apoptosis-related protein were detected by Western blot.

Results: GA enhanced the cytotoxicity of SMMC-7721 cells in a dose-dependent manner. The Fe3O4-MNP itself had no obviously inhibitory effect, but it could enhance the effect of GA on proliferation of SMMC-7721 cells. The apoptotic rate of SMMC-7721 cells induced by combination of GA with Fe3O4-MNP was higher than that by GA alone. The expression levels of caspase-3 and caspase-8 after co-treatment of GA and Fe3O4-MNP were higher than that exposed to either GA or Fe3O4-MNP alone, while the levels of bcl-2 were downregulated.

Conclusion: Fe3O4-MNP can promote GA-induced apoptosis of SMMC-7721 cells, which may be related to the downregulation of Bcl-2 and upregulation of caspase-3.

No MeSH data available.


Inhibitory effects of the combination of 20 μg/mL Fe3O4-MNP with different concentrations of GA on growth of SMMC-7721 cells by MTT assay for 48 h.Note: *P<0.05.Abbreviations: Fe3O4-MNP, magnetic nanoparticles of Fe3O4; GA, gambogic acid; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; h, hours.
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f4-ott-8-2285: Inhibitory effects of the combination of 20 μg/mL Fe3O4-MNP with different concentrations of GA on growth of SMMC-7721 cells by MTT assay for 48 h.Note: *P<0.05.Abbreviations: Fe3O4-MNP, magnetic nanoparticles of Fe3O4; GA, gambogic acid; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; h, hours.

Mentions: We noted that Fe3O4-MNP at less than 20 μg/mL concentration had no obviously effect on SMMC-7721 proliferation (P>0.05) (Figure 3). GA enhanced the inhibition of proliferation on SMMC-7721 cells in a dose manner. The IC50 value after incubation with GA for 48 hours was 2.40±0.11 μmol/L (P<0.05). Then we investigated the combination of 20 μg/mL Fe3O4-MNP with different concentrations of GA to find the optimal combination to affect SMMC-7721 cells. It was observed that the addition of Fe3O4-MNP could indeed increase the inhibition of SMMC-7721 cells with GA, and 20 μg/mL Fe3O4-MNP could decrease the IC50 value of GA to 1.29±0.07 μmol/L for 48 hours (P<0.05) (Figure 4).


Effects of magnetic nanoparticles of Fe3O4 combinated with gambogic acid on apoptosis of SMMC-7721 cells.

Tian L, Chen BA, Cheng J, Guo QL - Onco Targets Ther (2015)

Inhibitory effects of the combination of 20 μg/mL Fe3O4-MNP with different concentrations of GA on growth of SMMC-7721 cells by MTT assay for 48 h.Note: *P<0.05.Abbreviations: Fe3O4-MNP, magnetic nanoparticles of Fe3O4; GA, gambogic acid; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; h, hours.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556044&req=5

f4-ott-8-2285: Inhibitory effects of the combination of 20 μg/mL Fe3O4-MNP with different concentrations of GA on growth of SMMC-7721 cells by MTT assay for 48 h.Note: *P<0.05.Abbreviations: Fe3O4-MNP, magnetic nanoparticles of Fe3O4; GA, gambogic acid; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; h, hours.
Mentions: We noted that Fe3O4-MNP at less than 20 μg/mL concentration had no obviously effect on SMMC-7721 proliferation (P>0.05) (Figure 3). GA enhanced the inhibition of proliferation on SMMC-7721 cells in a dose manner. The IC50 value after incubation with GA for 48 hours was 2.40±0.11 μmol/L (P<0.05). Then we investigated the combination of 20 μg/mL Fe3O4-MNP with different concentrations of GA to find the optimal combination to affect SMMC-7721 cells. It was observed that the addition of Fe3O4-MNP could indeed increase the inhibition of SMMC-7721 cells with GA, and 20 μg/mL Fe3O4-MNP could decrease the IC50 value of GA to 1.29±0.07 μmol/L for 48 hours (P<0.05) (Figure 4).

Bottom Line: This study aims to investigate the potential benefit of combination therapy with magnetic nanoparticles of Fe3O4 (Fe3O4-MNP) and gambogic acid (GA) on SMMC-7721 cells.The Fe3O4-MNP itself had no obviously inhibitory effect, but it could enhance the effect of GA on proliferation of SMMC-7721 cells.Fe3O4-MNP can promote GA-induced apoptosis of SMMC-7721 cells, which may be related to the downregulation of Bcl-2 and upregulation of caspase-3.

View Article: PubMed Central - PubMed

Affiliation: Department of Hematology and Oncology (Key Department of Jiangsu Medicine), The Affiliated Zhongda Hospital, Medical School, Southeast University, Nanjing, Jiangsu, People's Republic of China.

ABSTRACT

Objective: This study aims to investigate the potential benefit of combination therapy with magnetic nanoparticles of Fe3O4 (Fe3O4-MNP) and gambogic acid (GA) on SMMC-7721 cells.

Methods: The inhibition of proliferation of SMMC-7721 cells was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Cell apoptosis was calculated and analyzed by flow cytometry, and the expressions of the apoptosis-related protein were detected by Western blot.

Results: GA enhanced the cytotoxicity of SMMC-7721 cells in a dose-dependent manner. The Fe3O4-MNP itself had no obviously inhibitory effect, but it could enhance the effect of GA on proliferation of SMMC-7721 cells. The apoptotic rate of SMMC-7721 cells induced by combination of GA with Fe3O4-MNP was higher than that by GA alone. The expression levels of caspase-3 and caspase-8 after co-treatment of GA and Fe3O4-MNP were higher than that exposed to either GA or Fe3O4-MNP alone, while the levels of bcl-2 were downregulated.

Conclusion: Fe3O4-MNP can promote GA-induced apoptosis of SMMC-7721 cells, which may be related to the downregulation of Bcl-2 and upregulation of caspase-3.

No MeSH data available.