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Effects of magnetic nanoparticles of Fe3O4 combinated with gambogic acid on apoptosis of SMMC-7721 cells.

Tian L, Chen BA, Cheng J, Guo QL - Onco Targets Ther (2015)

Bottom Line: The apoptotic rate of SMMC-7721 cells induced by combination of GA with Fe3O4-MNP was higher than that by GA alone.The expression levels of caspase-3 and caspase-8 after co-treatment of GA and Fe3O4-MNP were higher than that exposed to either GA or Fe3O4-MNP alone, while the levels of bcl-2 were downregulated.Fe3O4-MNP can promote GA-induced apoptosis of SMMC-7721 cells, which may be related to the downregulation of Bcl-2 and upregulation of caspase-3.

View Article: PubMed Central - PubMed

Affiliation: Department of Hematology and Oncology (Key Department of Jiangsu Medicine), The Affiliated Zhongda Hospital, Medical School, Southeast University, Nanjing, Jiangsu, People's Republic of China.

ABSTRACT

Objective: This study aims to investigate the potential benefit of combination therapy with magnetic nanoparticles of Fe3O4 (Fe3O4-MNP) and gambogic acid (GA) on SMMC-7721 cells.

Methods: The inhibition of proliferation of SMMC-7721 cells was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Cell apoptosis was calculated and analyzed by flow cytometry, and the expressions of the apoptosis-related protein were detected by Western blot.

Results: GA enhanced the cytotoxicity of SMMC-7721 cells in a dose-dependent manner. The Fe3O4-MNP itself had no obviously inhibitory effect, but it could enhance the effect of GA on proliferation of SMMC-7721 cells. The apoptotic rate of SMMC-7721 cells induced by combination of GA with Fe3O4-MNP was higher than that by GA alone. The expression levels of caspase-3 and caspase-8 after co-treatment of GA and Fe3O4-MNP were higher than that exposed to either GA or Fe3O4-MNP alone, while the levels of bcl-2 were downregulated.

Conclusion: Fe3O4-MNP can promote GA-induced apoptosis of SMMC-7721 cells, which may be related to the downregulation of Bcl-2 and upregulation of caspase-3.

No MeSH data available.


Related in: MedlinePlus

Molecular structure of GA.Abbreviation: GA, gambogic acid.
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f1-ott-8-2285: Molecular structure of GA.Abbreviation: GA, gambogic acid.

Mentions: Gambogic acid (GA; C38H44O8) (Figure 1) is one of active components of gamboge, a traditional Chinese medicine, which is a dry resin secreted from Garcinia hanburyi.1 It is reported in traditional Chinese medicine that GA is cold, acidic, acerbic, and poisonous. In addition, GA has a significant anticancer effect on a wide variety of solid tumors, including lymphoma, colorectal cancer, and glioblastoma.2–4 However, the molecular mechanisms of its antitumor activity and its effects on hepatocellular carcinoma (HCC) cells are still poorly understood and await further investigations.5


Effects of magnetic nanoparticles of Fe3O4 combinated with gambogic acid on apoptosis of SMMC-7721 cells.

Tian L, Chen BA, Cheng J, Guo QL - Onco Targets Ther (2015)

Molecular structure of GA.Abbreviation: GA, gambogic acid.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556044&req=5

f1-ott-8-2285: Molecular structure of GA.Abbreviation: GA, gambogic acid.
Mentions: Gambogic acid (GA; C38H44O8) (Figure 1) is one of active components of gamboge, a traditional Chinese medicine, which is a dry resin secreted from Garcinia hanburyi.1 It is reported in traditional Chinese medicine that GA is cold, acidic, acerbic, and poisonous. In addition, GA has a significant anticancer effect on a wide variety of solid tumors, including lymphoma, colorectal cancer, and glioblastoma.2–4 However, the molecular mechanisms of its antitumor activity and its effects on hepatocellular carcinoma (HCC) cells are still poorly understood and await further investigations.5

Bottom Line: The apoptotic rate of SMMC-7721 cells induced by combination of GA with Fe3O4-MNP was higher than that by GA alone.The expression levels of caspase-3 and caspase-8 after co-treatment of GA and Fe3O4-MNP were higher than that exposed to either GA or Fe3O4-MNP alone, while the levels of bcl-2 were downregulated.Fe3O4-MNP can promote GA-induced apoptosis of SMMC-7721 cells, which may be related to the downregulation of Bcl-2 and upregulation of caspase-3.

View Article: PubMed Central - PubMed

Affiliation: Department of Hematology and Oncology (Key Department of Jiangsu Medicine), The Affiliated Zhongda Hospital, Medical School, Southeast University, Nanjing, Jiangsu, People's Republic of China.

ABSTRACT

Objective: This study aims to investigate the potential benefit of combination therapy with magnetic nanoparticles of Fe3O4 (Fe3O4-MNP) and gambogic acid (GA) on SMMC-7721 cells.

Methods: The inhibition of proliferation of SMMC-7721 cells was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Cell apoptosis was calculated and analyzed by flow cytometry, and the expressions of the apoptosis-related protein were detected by Western blot.

Results: GA enhanced the cytotoxicity of SMMC-7721 cells in a dose-dependent manner. The Fe3O4-MNP itself had no obviously inhibitory effect, but it could enhance the effect of GA on proliferation of SMMC-7721 cells. The apoptotic rate of SMMC-7721 cells induced by combination of GA with Fe3O4-MNP was higher than that by GA alone. The expression levels of caspase-3 and caspase-8 after co-treatment of GA and Fe3O4-MNP were higher than that exposed to either GA or Fe3O4-MNP alone, while the levels of bcl-2 were downregulated.

Conclusion: Fe3O4-MNP can promote GA-induced apoptosis of SMMC-7721 cells, which may be related to the downregulation of Bcl-2 and upregulation of caspase-3.

No MeSH data available.


Related in: MedlinePlus