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Expressions and clinical significance of autophagy-related markers Beclin1, LC3, and EGFR in human cervical squamous cell carcinoma.

Hu YF, Lei X, Zhang HY, Ma JW, Yang WW, Chen ML, Cui J, Zhao H - Onco Targets Ther (2015)

Bottom Line: The protein expression rates were analyzed with χ (2) and Fisher's exact tests.Differences in overall survival (OS) were determined using the Kaplan-Meier method and log-rank tests.Beclin1- or LC3-negativity with EGFR-positivity in cervical SCC was associated with a higher Federation International of Gynecology and Obstetrics (FIGO) stage (P=0.011 and P=0.013, respectively) and pelvic lymph node metastasis (P=0.036 and P=0.092, respectively).

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Yan'an University Affiliated Hospital, Yan'an, Shaanxi Province, People's Republic of China.

ABSTRACT

Purpose: We aimed to investigate the expression of EGFR and the autophagy-related markers Beclin1 and LC3 in cervical cancer.

Methods: Beclin1, LC3, and EGFR expression were analyzed in 80 samples of cervical squamous cell carcinoma (SCC), 40 samples of high-grade cervical intraepithelial neoplasia (CIN), and 40 samples of normal cervical tissues by immunohistochemistry. The protein expression rates were analyzed with χ (2) and Fisher's exact tests. Differences in overall survival (OS) were determined using the Kaplan-Meier method and log-rank tests.

Results: Cervical cancer, high-grade CIN, and normal cervical epithelial cells expressed Beclin1 in 26.2%, 77.5%, and 82.5% of patients, respectively, and expressed LC3 in 28.8%, 70.0%, and 75.0% of patients, respectively. There was a significant difference between cervical SCC and high-grade CIN or normal cervical epithelial cells (P=0.000). Cervical cancer cells, high-grade CIN cells, and normal cervical epithelial cells expressed EGFR in 68.8%, 62.5%, and 12.5% of patients, respectively. There was a significant difference between cervical SCC or high-grade CIN and normal cervical epithelial cells (P=0.000). No significant association between Beclin1 or LC3 or EGFR expression and various clinicopathological parameters was observed in cervical SCC. There was no significant correlation between Beclin1, LC3, EGFR expression, and 5-year OS rates of cervical SCC patients. Beclin1- or LC3-negativity with EGFR-positivity in cervical SCC was associated with a higher Federation International of Gynecology and Obstetrics (FIGO) stage (P=0.011 and P=0.013, respectively) and pelvic lymph node metastasis (P=0.036 and P=0.092, respectively). The 5-year OS rates did not significantly differ between Beclin1- or LC3-positive and -negative patients with positive EGFR.

Conclusion: Autophagy was downregulated and EGFR was upregulated in cervical SCC. Autophagy downregulation combined with EGFR upregulation promotes the progression of cervical SCC.

No MeSH data available.


Related in: MedlinePlus

The univariate survival analyses with Kaplan–Meier method and log-rank test. There were no significant differences in the 5-year OS rate between the Beclin1- or LC3- or EGFR-positive and -negative patients with cervical SCC. (A–C) There was no significant difference in the 5-year OS rate between the Beclin1- or LC3-positive and -negative patients with positive EGFR expression (D and E).Abbreviations: OS, overall survival; SCC, squamous cell carcinoma; NS, not significant.
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f2-ott-8-2243: The univariate survival analyses with Kaplan–Meier method and log-rank test. There were no significant differences in the 5-year OS rate between the Beclin1- or LC3- or EGFR-positive and -negative patients with cervical SCC. (A–C) There was no significant difference in the 5-year OS rate between the Beclin1- or LC3-positive and -negative patients with positive EGFR expression (D and E).Abbreviations: OS, overall survival; SCC, squamous cell carcinoma; NS, not significant.

Mentions: The average duration of follow up was 63.5 months (range 8–79 months). The 5-year OS rates of Beclin1-negative and -positive patients were 71.2% and 85.7%, respectively (χ2=1.69, P=0.194) (Figure 2A). The 5-year OS rates of LC3-negative and -positive patients were 71.9% and 82.6%, respectively (χ2=0.889, P=0.346) (Figure 2B). The 5-year OS rates of EGFR-negative and -positive patients were 88.0% and 69.1%, respectively (χ2=3.182, P=0.074) (Figure 2C). The 5-year OS rates of Beclin1-negative and -positive patients with positive EGFR were 64.1% and 81.3%, respectively (χ2=1.482, P=0.224) (Figure 2D). The 5-year OS rates of LC3-positive and -negative patients with positive EGFR were 64.3% and 84.6%, respectively (χ2=1.693, P=0.193) (Figure 2E).


Expressions and clinical significance of autophagy-related markers Beclin1, LC3, and EGFR in human cervical squamous cell carcinoma.

Hu YF, Lei X, Zhang HY, Ma JW, Yang WW, Chen ML, Cui J, Zhao H - Onco Targets Ther (2015)

The univariate survival analyses with Kaplan–Meier method and log-rank test. There were no significant differences in the 5-year OS rate between the Beclin1- or LC3- or EGFR-positive and -negative patients with cervical SCC. (A–C) There was no significant difference in the 5-year OS rate between the Beclin1- or LC3-positive and -negative patients with positive EGFR expression (D and E).Abbreviations: OS, overall survival; SCC, squamous cell carcinoma; NS, not significant.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4556028&req=5

f2-ott-8-2243: The univariate survival analyses with Kaplan–Meier method and log-rank test. There were no significant differences in the 5-year OS rate between the Beclin1- or LC3- or EGFR-positive and -negative patients with cervical SCC. (A–C) There was no significant difference in the 5-year OS rate between the Beclin1- or LC3-positive and -negative patients with positive EGFR expression (D and E).Abbreviations: OS, overall survival; SCC, squamous cell carcinoma; NS, not significant.
Mentions: The average duration of follow up was 63.5 months (range 8–79 months). The 5-year OS rates of Beclin1-negative and -positive patients were 71.2% and 85.7%, respectively (χ2=1.69, P=0.194) (Figure 2A). The 5-year OS rates of LC3-negative and -positive patients were 71.9% and 82.6%, respectively (χ2=0.889, P=0.346) (Figure 2B). The 5-year OS rates of EGFR-negative and -positive patients were 88.0% and 69.1%, respectively (χ2=3.182, P=0.074) (Figure 2C). The 5-year OS rates of Beclin1-negative and -positive patients with positive EGFR were 64.1% and 81.3%, respectively (χ2=1.482, P=0.224) (Figure 2D). The 5-year OS rates of LC3-positive and -negative patients with positive EGFR were 64.3% and 84.6%, respectively (χ2=1.693, P=0.193) (Figure 2E).

Bottom Line: The protein expression rates were analyzed with χ (2) and Fisher's exact tests.Differences in overall survival (OS) were determined using the Kaplan-Meier method and log-rank tests.Beclin1- or LC3-negativity with EGFR-positivity in cervical SCC was associated with a higher Federation International of Gynecology and Obstetrics (FIGO) stage (P=0.011 and P=0.013, respectively) and pelvic lymph node metastasis (P=0.036 and P=0.092, respectively).

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Yan'an University Affiliated Hospital, Yan'an, Shaanxi Province, People's Republic of China.

ABSTRACT

Purpose: We aimed to investigate the expression of EGFR and the autophagy-related markers Beclin1 and LC3 in cervical cancer.

Methods: Beclin1, LC3, and EGFR expression were analyzed in 80 samples of cervical squamous cell carcinoma (SCC), 40 samples of high-grade cervical intraepithelial neoplasia (CIN), and 40 samples of normal cervical tissues by immunohistochemistry. The protein expression rates were analyzed with χ (2) and Fisher's exact tests. Differences in overall survival (OS) were determined using the Kaplan-Meier method and log-rank tests.

Results: Cervical cancer, high-grade CIN, and normal cervical epithelial cells expressed Beclin1 in 26.2%, 77.5%, and 82.5% of patients, respectively, and expressed LC3 in 28.8%, 70.0%, and 75.0% of patients, respectively. There was a significant difference between cervical SCC and high-grade CIN or normal cervical epithelial cells (P=0.000). Cervical cancer cells, high-grade CIN cells, and normal cervical epithelial cells expressed EGFR in 68.8%, 62.5%, and 12.5% of patients, respectively. There was a significant difference between cervical SCC or high-grade CIN and normal cervical epithelial cells (P=0.000). No significant association between Beclin1 or LC3 or EGFR expression and various clinicopathological parameters was observed in cervical SCC. There was no significant correlation between Beclin1, LC3, EGFR expression, and 5-year OS rates of cervical SCC patients. Beclin1- or LC3-negativity with EGFR-positivity in cervical SCC was associated with a higher Federation International of Gynecology and Obstetrics (FIGO) stage (P=0.011 and P=0.013, respectively) and pelvic lymph node metastasis (P=0.036 and P=0.092, respectively). The 5-year OS rates did not significantly differ between Beclin1- or LC3-positive and -negative patients with positive EGFR.

Conclusion: Autophagy was downregulated and EGFR was upregulated in cervical SCC. Autophagy downregulation combined with EGFR upregulation promotes the progression of cervical SCC.

No MeSH data available.


Related in: MedlinePlus