Limits...
Expressions and clinical significance of autophagy-related markers Beclin1, LC3, and EGFR in human cervical squamous cell carcinoma.

Hu YF, Lei X, Zhang HY, Ma JW, Yang WW, Chen ML, Cui J, Zhao H - Onco Targets Ther (2015)

Bottom Line: The protein expression rates were analyzed with χ (2) and Fisher's exact tests.Differences in overall survival (OS) were determined using the Kaplan-Meier method and log-rank tests.Beclin1- or LC3-negativity with EGFR-positivity in cervical SCC was associated with a higher Federation International of Gynecology and Obstetrics (FIGO) stage (P=0.011 and P=0.013, respectively) and pelvic lymph node metastasis (P=0.036 and P=0.092, respectively).

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Yan'an University Affiliated Hospital, Yan'an, Shaanxi Province, People's Republic of China.

ABSTRACT

Purpose: We aimed to investigate the expression of EGFR and the autophagy-related markers Beclin1 and LC3 in cervical cancer.

Methods: Beclin1, LC3, and EGFR expression were analyzed in 80 samples of cervical squamous cell carcinoma (SCC), 40 samples of high-grade cervical intraepithelial neoplasia (CIN), and 40 samples of normal cervical tissues by immunohistochemistry. The protein expression rates were analyzed with χ (2) and Fisher's exact tests. Differences in overall survival (OS) were determined using the Kaplan-Meier method and log-rank tests.

Results: Cervical cancer, high-grade CIN, and normal cervical epithelial cells expressed Beclin1 in 26.2%, 77.5%, and 82.5% of patients, respectively, and expressed LC3 in 28.8%, 70.0%, and 75.0% of patients, respectively. There was a significant difference between cervical SCC and high-grade CIN or normal cervical epithelial cells (P=0.000). Cervical cancer cells, high-grade CIN cells, and normal cervical epithelial cells expressed EGFR in 68.8%, 62.5%, and 12.5% of patients, respectively. There was a significant difference between cervical SCC or high-grade CIN and normal cervical epithelial cells (P=0.000). No significant association between Beclin1 or LC3 or EGFR expression and various clinicopathological parameters was observed in cervical SCC. There was no significant correlation between Beclin1, LC3, EGFR expression, and 5-year OS rates of cervical SCC patients. Beclin1- or LC3-negativity with EGFR-positivity in cervical SCC was associated with a higher Federation International of Gynecology and Obstetrics (FIGO) stage (P=0.011 and P=0.013, respectively) and pelvic lymph node metastasis (P=0.036 and P=0.092, respectively). The 5-year OS rates did not significantly differ between Beclin1- or LC3-positive and -negative patients with positive EGFR.

Conclusion: Autophagy was downregulated and EGFR was upregulated in cervical SCC. Autophagy downregulation combined with EGFR upregulation promotes the progression of cervical SCC.

No MeSH data available.


Related in: MedlinePlus

Representative immunohistochemistry micrographs for Beclin1, LC3, and EGFR expression in cervical SCC, high-grade CIN, and normal cervical tissues.Notes: (A) HE staining of normal cervical tissues. (B) HE staining of high-grade CIN. (C) HE staining of cervical SCC. (D) Moderate positivity of Beclin1 in normal cervical tissues. (E) Moderate positivity of Beclin1 in high-grade CIN. (F) Moderate positivity of Beclin1 in cervical SCC. (G) Moderate positivity of LC3 in normal cervical tissues. (H) Moderate positivity of LC3 in high-grade CIN. (I) Weak positivity of LC3 in cervical SCC. (J) Weak positivity of EGFR in normal cervical tissues. (K) Moderate positivity of EGFR in high-grade CIN. (L) Strong positivity of EGFR in cervical SCC. Original magnification ×40 (A–C) and ×200 (D–L).Abbreviations: CIN, cervical intraepithelial neoplasia; HE, hematoxylin and eosin; SCC, squamous cell carcinoma.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4556028&req=5

f1-ott-8-2243: Representative immunohistochemistry micrographs for Beclin1, LC3, and EGFR expression in cervical SCC, high-grade CIN, and normal cervical tissues.Notes: (A) HE staining of normal cervical tissues. (B) HE staining of high-grade CIN. (C) HE staining of cervical SCC. (D) Moderate positivity of Beclin1 in normal cervical tissues. (E) Moderate positivity of Beclin1 in high-grade CIN. (F) Moderate positivity of Beclin1 in cervical SCC. (G) Moderate positivity of LC3 in normal cervical tissues. (H) Moderate positivity of LC3 in high-grade CIN. (I) Weak positivity of LC3 in cervical SCC. (J) Weak positivity of EGFR in normal cervical tissues. (K) Moderate positivity of EGFR in high-grade CIN. (L) Strong positivity of EGFR in cervical SCC. Original magnification ×40 (A–C) and ×200 (D–L).Abbreviations: CIN, cervical intraepithelial neoplasia; HE, hematoxylin and eosin; SCC, squamous cell carcinoma.

Mentions: Specific EGFR staining was observed mainly in the cytoplasm or cell membrane. Cervical cancer cells, high-grade CIN cells, and normal cervical epithelial cells expressed EGFR in 68.8% (55/80), 62.5% (25/40), and 12.5% (5/40) of patients, respectively (Table 1; Figure 1). There was a significant difference between cervical SCC or high-grade CIN and normal cervical epithelial cells (P=0.000) (Table 1; Figure 1).


Expressions and clinical significance of autophagy-related markers Beclin1, LC3, and EGFR in human cervical squamous cell carcinoma.

Hu YF, Lei X, Zhang HY, Ma JW, Yang WW, Chen ML, Cui J, Zhao H - Onco Targets Ther (2015)

Representative immunohistochemistry micrographs for Beclin1, LC3, and EGFR expression in cervical SCC, high-grade CIN, and normal cervical tissues.Notes: (A) HE staining of normal cervical tissues. (B) HE staining of high-grade CIN. (C) HE staining of cervical SCC. (D) Moderate positivity of Beclin1 in normal cervical tissues. (E) Moderate positivity of Beclin1 in high-grade CIN. (F) Moderate positivity of Beclin1 in cervical SCC. (G) Moderate positivity of LC3 in normal cervical tissues. (H) Moderate positivity of LC3 in high-grade CIN. (I) Weak positivity of LC3 in cervical SCC. (J) Weak positivity of EGFR in normal cervical tissues. (K) Moderate positivity of EGFR in high-grade CIN. (L) Strong positivity of EGFR in cervical SCC. Original magnification ×40 (A–C) and ×200 (D–L).Abbreviations: CIN, cervical intraepithelial neoplasia; HE, hematoxylin and eosin; SCC, squamous cell carcinoma.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556028&req=5

f1-ott-8-2243: Representative immunohistochemistry micrographs for Beclin1, LC3, and EGFR expression in cervical SCC, high-grade CIN, and normal cervical tissues.Notes: (A) HE staining of normal cervical tissues. (B) HE staining of high-grade CIN. (C) HE staining of cervical SCC. (D) Moderate positivity of Beclin1 in normal cervical tissues. (E) Moderate positivity of Beclin1 in high-grade CIN. (F) Moderate positivity of Beclin1 in cervical SCC. (G) Moderate positivity of LC3 in normal cervical tissues. (H) Moderate positivity of LC3 in high-grade CIN. (I) Weak positivity of LC3 in cervical SCC. (J) Weak positivity of EGFR in normal cervical tissues. (K) Moderate positivity of EGFR in high-grade CIN. (L) Strong positivity of EGFR in cervical SCC. Original magnification ×40 (A–C) and ×200 (D–L).Abbreviations: CIN, cervical intraepithelial neoplasia; HE, hematoxylin and eosin; SCC, squamous cell carcinoma.
Mentions: Specific EGFR staining was observed mainly in the cytoplasm or cell membrane. Cervical cancer cells, high-grade CIN cells, and normal cervical epithelial cells expressed EGFR in 68.8% (55/80), 62.5% (25/40), and 12.5% (5/40) of patients, respectively (Table 1; Figure 1). There was a significant difference between cervical SCC or high-grade CIN and normal cervical epithelial cells (P=0.000) (Table 1; Figure 1).

Bottom Line: The protein expression rates were analyzed with χ (2) and Fisher's exact tests.Differences in overall survival (OS) were determined using the Kaplan-Meier method and log-rank tests.Beclin1- or LC3-negativity with EGFR-positivity in cervical SCC was associated with a higher Federation International of Gynecology and Obstetrics (FIGO) stage (P=0.011 and P=0.013, respectively) and pelvic lymph node metastasis (P=0.036 and P=0.092, respectively).

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Yan'an University Affiliated Hospital, Yan'an, Shaanxi Province, People's Republic of China.

ABSTRACT

Purpose: We aimed to investigate the expression of EGFR and the autophagy-related markers Beclin1 and LC3 in cervical cancer.

Methods: Beclin1, LC3, and EGFR expression were analyzed in 80 samples of cervical squamous cell carcinoma (SCC), 40 samples of high-grade cervical intraepithelial neoplasia (CIN), and 40 samples of normal cervical tissues by immunohistochemistry. The protein expression rates were analyzed with χ (2) and Fisher's exact tests. Differences in overall survival (OS) were determined using the Kaplan-Meier method and log-rank tests.

Results: Cervical cancer, high-grade CIN, and normal cervical epithelial cells expressed Beclin1 in 26.2%, 77.5%, and 82.5% of patients, respectively, and expressed LC3 in 28.8%, 70.0%, and 75.0% of patients, respectively. There was a significant difference between cervical SCC and high-grade CIN or normal cervical epithelial cells (P=0.000). Cervical cancer cells, high-grade CIN cells, and normal cervical epithelial cells expressed EGFR in 68.8%, 62.5%, and 12.5% of patients, respectively. There was a significant difference between cervical SCC or high-grade CIN and normal cervical epithelial cells (P=0.000). No significant association between Beclin1 or LC3 or EGFR expression and various clinicopathological parameters was observed in cervical SCC. There was no significant correlation between Beclin1, LC3, EGFR expression, and 5-year OS rates of cervical SCC patients. Beclin1- or LC3-negativity with EGFR-positivity in cervical SCC was associated with a higher Federation International of Gynecology and Obstetrics (FIGO) stage (P=0.011 and P=0.013, respectively) and pelvic lymph node metastasis (P=0.036 and P=0.092, respectively). The 5-year OS rates did not significantly differ between Beclin1- or LC3-positive and -negative patients with positive EGFR.

Conclusion: Autophagy was downregulated and EGFR was upregulated in cervical SCC. Autophagy downregulation combined with EGFR upregulation promotes the progression of cervical SCC.

No MeSH data available.


Related in: MedlinePlus