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Molecular characterization of carotenoid biosynthetic genes and carotenoid accumulation in Scutellaria baicalensis Georgi.

Tuan PA, Kim YB, Kim JK, Arasu MV, Al-Dhabi NA, Park SU - EXCLI J (2014)

Bottom Line: SbCHXB and SbZEP transcripts were expressed at relatively high levels in the roots, stems, and flowers and were expressed at low levels in the leaves, where carotenoids were mostly distributed.The predominant carotenoids in S.b aicalensiswere lutein and β-carotene, with abundant amounts found in the leaves (517.19 and 228.37 μg g(-1) dry weight, respectively).Our study on the biosynthesis of carotenoids in S. baicalensis will provide basic data for elucidating the contribution of carotenoids to the considerable medicinal properties of S. baicalensis.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Science, Chungnam National University, 99 Daehak-Ro, Yuseong-gu, Daejeon 305-764, Korea.

ABSTRACT
Scutellaria baicalensis has a wide range of biological activities and has been considered as an important traditional drug in Asia and North America for centuries. A partial-length cDNA clone encoding phytoene synthase (SbPSY) and full-length cDNA clonesencoding phytoene desaturase (SbPDS), ξ-carotene desaturase (SbZDS), β-ring carotene hydroxylase (SbCHXB), and zeaxanthin epoxidase (SbZEP)were identifiedin S. baicalensis. Sequence analyses revealed that these proteins share high identity and conserved domains with their orthologous genes. SbPSY, SbPDS, SbZDS, SbCHXB, and SbZEP were constitutively expressed in the roots, stems, leaves, and flowers of S.b aicalensis. SbPSY, SbPDS, and SbZDS were highly expressed in the stems, leaves, and flowers and showed low expression in the roots, where only trace amounts of carotenoids were detected. SbCHXB and SbZEP transcripts were expressed at relatively high levels in the roots, stems, and flowers and were expressed at low levels in the leaves, where carotenoids were mostly distributed. The predominant carotenoids in S.b aicalensiswere lutein and β-carotene, with abundant amounts found in the leaves (517.19 and 228.37 μg g(-1) dry weight, respectively). Our study on the biosynthesis of carotenoids in S. baicalensis will provide basic data for elucidating the contribution of carotenoids to the considerable medicinal properties of S. baicalensis.

No MeSH data available.


Multiple alignments of the amino acid sequences of SbCHXB with other CHXBs. Identical residues are indicated by a black background, and similar residues are shaded with a gray background. HXXXH and HXXHH, where X encodes for any amino acid, represent 4 conservatively spaced histidine motifs. InCHXB, Ipomoea nil (AB499058); VvCHXB, Vitis vinifera (XM_002273545); CaCHXB, Coffea arabica (DQ157169); LeCHXB, Lycopersicon esculentum (DQ864755).
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Figure 5: Multiple alignments of the amino acid sequences of SbCHXB with other CHXBs. Identical residues are indicated by a black background, and similar residues are shaded with a gray background. HXXXH and HXXHH, where X encodes for any amino acid, represent 4 conservatively spaced histidine motifs. InCHXB, Ipomoea nil (AB499058); VvCHXB, Vitis vinifera (XM_002273545); CaCHXB, Coffea arabica (DQ157169); LeCHXB, Lycopersicon esculentum (DQ864755).

Mentions: SbCHXB consisted of 1410 bp, with a 939-bp ORF encoding a protein of 312 amino acids (predicted molecular mass of 34.48 kDa; Figure 5(Fig. 5)). SbCHXB exhibited 75 % identity and 84 % similarity with Ipomoea nil CHXB, 76 % identity and 83 % similarity with Vitis vinifera CHXB, 78 % identity and 85 % similarity with C. arabica CHXB, and 74 % identity and 82 % similarity with Lycopersicon esculentum CHXB. Four conservatively spaced histidine motifs proposed to be involved in iron binding during hydroxylation reactions are presented in Figure 5(Fig. 5) (Bouvier et al., 1998[3]).


Molecular characterization of carotenoid biosynthetic genes and carotenoid accumulation in Scutellaria baicalensis Georgi.

Tuan PA, Kim YB, Kim JK, Arasu MV, Al-Dhabi NA, Park SU - EXCLI J (2014)

Multiple alignments of the amino acid sequences of SbCHXB with other CHXBs. Identical residues are indicated by a black background, and similar residues are shaded with a gray background. HXXXH and HXXHH, where X encodes for any amino acid, represent 4 conservatively spaced histidine motifs. InCHXB, Ipomoea nil (AB499058); VvCHXB, Vitis vinifera (XM_002273545); CaCHXB, Coffea arabica (DQ157169); LeCHXB, Lycopersicon esculentum (DQ864755).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4556017&req=5

Figure 5: Multiple alignments of the amino acid sequences of SbCHXB with other CHXBs. Identical residues are indicated by a black background, and similar residues are shaded with a gray background. HXXXH and HXXHH, where X encodes for any amino acid, represent 4 conservatively spaced histidine motifs. InCHXB, Ipomoea nil (AB499058); VvCHXB, Vitis vinifera (XM_002273545); CaCHXB, Coffea arabica (DQ157169); LeCHXB, Lycopersicon esculentum (DQ864755).
Mentions: SbCHXB consisted of 1410 bp, with a 939-bp ORF encoding a protein of 312 amino acids (predicted molecular mass of 34.48 kDa; Figure 5(Fig. 5)). SbCHXB exhibited 75 % identity and 84 % similarity with Ipomoea nil CHXB, 76 % identity and 83 % similarity with Vitis vinifera CHXB, 78 % identity and 85 % similarity with C. arabica CHXB, and 74 % identity and 82 % similarity with Lycopersicon esculentum CHXB. Four conservatively spaced histidine motifs proposed to be involved in iron binding during hydroxylation reactions are presented in Figure 5(Fig. 5) (Bouvier et al., 1998[3]).

Bottom Line: SbCHXB and SbZEP transcripts were expressed at relatively high levels in the roots, stems, and flowers and were expressed at low levels in the leaves, where carotenoids were mostly distributed.The predominant carotenoids in S.b aicalensiswere lutein and β-carotene, with abundant amounts found in the leaves (517.19 and 228.37 μg g(-1) dry weight, respectively).Our study on the biosynthesis of carotenoids in S. baicalensis will provide basic data for elucidating the contribution of carotenoids to the considerable medicinal properties of S. baicalensis.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Science, Chungnam National University, 99 Daehak-Ro, Yuseong-gu, Daejeon 305-764, Korea.

ABSTRACT
Scutellaria baicalensis has a wide range of biological activities and has been considered as an important traditional drug in Asia and North America for centuries. A partial-length cDNA clone encoding phytoene synthase (SbPSY) and full-length cDNA clonesencoding phytoene desaturase (SbPDS), ξ-carotene desaturase (SbZDS), β-ring carotene hydroxylase (SbCHXB), and zeaxanthin epoxidase (SbZEP)were identifiedin S. baicalensis. Sequence analyses revealed that these proteins share high identity and conserved domains with their orthologous genes. SbPSY, SbPDS, SbZDS, SbCHXB, and SbZEP were constitutively expressed in the roots, stems, leaves, and flowers of S.b aicalensis. SbPSY, SbPDS, and SbZDS were highly expressed in the stems, leaves, and flowers and showed low expression in the roots, where only trace amounts of carotenoids were detected. SbCHXB and SbZEP transcripts were expressed at relatively high levels in the roots, stems, and flowers and were expressed at low levels in the leaves, where carotenoids were mostly distributed. The predominant carotenoids in S.b aicalensiswere lutein and β-carotene, with abundant amounts found in the leaves (517.19 and 228.37 μg g(-1) dry weight, respectively). Our study on the biosynthesis of carotenoids in S. baicalensis will provide basic data for elucidating the contribution of carotenoids to the considerable medicinal properties of S. baicalensis.

No MeSH data available.