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Evaluation of dual-wavelength excitation autofluorescence imaging of colorectal tumours with a high-sensitivity CMOS imager: a cross-sectional study.

Kominami Y, Yoshida S, Tanaka S, Miyaki R, Sanomura Y, Seo MW, Kagawa K, Kawahito S, Arimoto H, Yamada K, Chayama K - BMC Gastroenterol (2015)

Bottom Line: Ratio images (F365ex/F405ex) were created to evaluate the lesion brightness compared with that of normal mucosa, and specimens were categorized into a no signal or high signal group.Adenomas and adenocarcinomas were depicted in 87 ratio images, with 86.2% (n = 75) in the High signal group.SSA/P was depicted in 20 ratio images, with 70.0% (n = 14) in the High signal group.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology and Metabolism, Graduate School of Biomedical and Health Science, Hiroshima University, Hiroshima, Japan. kominami@hiroshima-u.ac.jp.

ABSTRACT

Background: It is important to devise efficient and easy methods of detecting colorectal tumours to reduce mortality from colorectal cancer. Dual-wavelength excitation autofluorescence intensity can be used to visualize colorectal tumours. Therefore, we evaluated dual-wavelength excitation autofluorescence images of colorectal tumours obtained with a newly developed, high-sensitivity complementary metal-oxide-semiconductor (CMOS) imager.

Methods: A total 107 colorectal tumours (44 adenomas, 43 adenocarcinomas with intramucosal invasion, and 20 sessile serrated adenoma/polyps [SSA/Ps]) in 98 patients who underwent endoscopic tumour resection were included. The specimens were irradiated with excitation light at 365 nm and 405 nm, and autofluorescence images measured with a 475 ± 25-nm band pass filter were obtained using a new, high-sensitivity CMOS imager. Ratio images (F365ex/F405ex) were created to evaluate the lesion brightness compared with that of normal mucosa, and specimens were categorized into a no signal or high signal group.

Results: Adenomas and adenocarcinomas were depicted in 87 ratio images, with 86.2% (n = 75) in the High signal group. SSA/P was depicted in 20 ratio images, with 70.0% (n = 14) in the High signal group.

Conclusions: Dual-wavelength excitation autofluorescence images of colorectal tumours can be acquired using our high-sensitivity CMOS imager, and are useful in detecting colorectal tumours.

No MeSH data available.


Related in: MedlinePlus

Representative autofluorescence ratio image. Autofluorescence images were obtained with a high-sensitivity CMOS imager by irradiating the resected specimen with 405-nm followed by 365-nm excitation light. The ratio images were then created by dividing the F365ex (365 nm) by F405ex (405 nm). a. Colon tumour observed during endoscopy. b. Fresh resected specimen. c. Autofluorescence image at 365-nm excitation (F365ex). d. Autofluorescence imaging at 405-nm excitation (F405ex). e. The calculated ratio image. f. Resected formalin-fixed specimen
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Fig1: Representative autofluorescence ratio image. Autofluorescence images were obtained with a high-sensitivity CMOS imager by irradiating the resected specimen with 405-nm followed by 365-nm excitation light. The ratio images were then created by dividing the F365ex (365 nm) by F405ex (405 nm). a. Colon tumour observed during endoscopy. b. Fresh resected specimen. c. Autofluorescence image at 365-nm excitation (F365ex). d. Autofluorescence imaging at 405-nm excitation (F405ex). e. The calculated ratio image. f. Resected formalin-fixed specimen

Mentions: Sequential autofluorescence surface images of specimens containing both tumour and normal tissue were obtained using a band pass filter (475 ± 25 nm) at a 365-nm excitation (F365ex) followed by a 405-nm excitation (F405ex), as shown in Fig. 1.Fig. 1


Evaluation of dual-wavelength excitation autofluorescence imaging of colorectal tumours with a high-sensitivity CMOS imager: a cross-sectional study.

Kominami Y, Yoshida S, Tanaka S, Miyaki R, Sanomura Y, Seo MW, Kagawa K, Kawahito S, Arimoto H, Yamada K, Chayama K - BMC Gastroenterol (2015)

Representative autofluorescence ratio image. Autofluorescence images were obtained with a high-sensitivity CMOS imager by irradiating the resected specimen with 405-nm followed by 365-nm excitation light. The ratio images were then created by dividing the F365ex (365 nm) by F405ex (405 nm). a. Colon tumour observed during endoscopy. b. Fresh resected specimen. c. Autofluorescence image at 365-nm excitation (F365ex). d. Autofluorescence imaging at 405-nm excitation (F405ex). e. The calculated ratio image. f. Resected formalin-fixed specimen
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4556006&req=5

Fig1: Representative autofluorescence ratio image. Autofluorescence images were obtained with a high-sensitivity CMOS imager by irradiating the resected specimen with 405-nm followed by 365-nm excitation light. The ratio images were then created by dividing the F365ex (365 nm) by F405ex (405 nm). a. Colon tumour observed during endoscopy. b. Fresh resected specimen. c. Autofluorescence image at 365-nm excitation (F365ex). d. Autofluorescence imaging at 405-nm excitation (F405ex). e. The calculated ratio image. f. Resected formalin-fixed specimen
Mentions: Sequential autofluorescence surface images of specimens containing both tumour and normal tissue were obtained using a band pass filter (475 ± 25 nm) at a 365-nm excitation (F365ex) followed by a 405-nm excitation (F405ex), as shown in Fig. 1.Fig. 1

Bottom Line: Ratio images (F365ex/F405ex) were created to evaluate the lesion brightness compared with that of normal mucosa, and specimens were categorized into a no signal or high signal group.Adenomas and adenocarcinomas were depicted in 87 ratio images, with 86.2% (n = 75) in the High signal group.SSA/P was depicted in 20 ratio images, with 70.0% (n = 14) in the High signal group.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology and Metabolism, Graduate School of Biomedical and Health Science, Hiroshima University, Hiroshima, Japan. kominami@hiroshima-u.ac.jp.

ABSTRACT

Background: It is important to devise efficient and easy methods of detecting colorectal tumours to reduce mortality from colorectal cancer. Dual-wavelength excitation autofluorescence intensity can be used to visualize colorectal tumours. Therefore, we evaluated dual-wavelength excitation autofluorescence images of colorectal tumours obtained with a newly developed, high-sensitivity complementary metal-oxide-semiconductor (CMOS) imager.

Methods: A total 107 colorectal tumours (44 adenomas, 43 adenocarcinomas with intramucosal invasion, and 20 sessile serrated adenoma/polyps [SSA/Ps]) in 98 patients who underwent endoscopic tumour resection were included. The specimens were irradiated with excitation light at 365 nm and 405 nm, and autofluorescence images measured with a 475 ± 25-nm band pass filter were obtained using a new, high-sensitivity CMOS imager. Ratio images (F365ex/F405ex) were created to evaluate the lesion brightness compared with that of normal mucosa, and specimens were categorized into a no signal or high signal group.

Results: Adenomas and adenocarcinomas were depicted in 87 ratio images, with 86.2% (n = 75) in the High signal group. SSA/P was depicted in 20 ratio images, with 70.0% (n = 14) in the High signal group.

Conclusions: Dual-wavelength excitation autofluorescence images of colorectal tumours can be acquired using our high-sensitivity CMOS imager, and are useful in detecting colorectal tumours.

No MeSH data available.


Related in: MedlinePlus