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Immunosuppressive effects of the standardized extract of Phyllanthus amarus on cellular immune responses in Wistar-Kyoto rats.

Ilangkovan M, Jantan I, Mesaik MA, Bukhari SN - Drug Des Devel Ther (2015)

Bottom Line: The results in P. amarus-treated rats also demonstrated a dose-dependent inhibition of both lipopolysaccharide-stimulated B-cell proliferation and concanavalin A-stimulated T-cell proliferation as compared with sensitized control.At a dose of 400 mg/kg (P<0.01), there was a significant decrease in the (%) expression of CD4(+) and CD8(+) in splenocytes and in serum cytokines of T helper (Th1) (IL-2 and IFN-γ) and Th2 (IL-4).In conclusion, P. amarus showed effective immunosuppressive activities in cellular immune response, by various immune regulatory mechanisms, and may be useful for improvement of immune-related disorders.

View Article: PubMed Central - PubMed

Affiliation: Drug and Herbal Research Center, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.

ABSTRACT
Phyllanthus amarus (family: Euphorbiaceae) is of immense interest due to its wide spectrum of biological activities. In the present study, the standardized 80% ethanol extract of P. amarus was investigated for its modulatory activity on various cellular immune parameters, including chemotaxis of neutrophils, engulfment of Escherichia coli by neutrophils, and Mac-1 expression, in leukocytes isolated from treated/nontreated Wistar-Kyoto rats. The detailed cell-mediated activity of P. amarus was also investigated, including analysis of the effects on T- and B-cell proliferation and CD4(+) and CD8(+) T-cell subsets in splenic mononuclear cells, and estimation of serum cytokine production by activated T-cells. The main components of the extract, phyllanthin, hypophyllanthin, corilagin, geraniin, ellagic acid, and gallic acid were identified and quantitatively analyzed in the extracts, using validated reversed-phase high-performance liquid chromatography (HPLC) methods. N-formyl-methionyl-leucyl-phenylalanine (fMLP)-induced neutrophils isolated from rats administered with the extract of P. amarus, at doses ranging from 100 to 400 mg/kg for 14 days, revealed a significant dose-dependent reduction in neutrophil migration (P<0.05). Similar patterns of inhibition were also observed in phagocytic activity and in fMLP-induced changes in expression of β2 integrin polymorphonuclear neutrophils. The results in P. amarus-treated rats also demonstrated a dose-dependent inhibition of both lipopolysaccharide-stimulated B-cell proliferation and concanavalin A-stimulated T-cell proliferation as compared with sensitized control. At a dose of 400 mg/kg (P<0.01), there was a significant decrease in the (%) expression of CD4(+) and CD8(+) in splenocytes and in serum cytokines of T helper (Th1) (IL-2 and IFN-γ) and Th2 (IL-4). In conclusion, P. amarus showed effective immunosuppressive activities in cellular immune response, by various immune regulatory mechanisms, and may be useful for improvement of immune-related disorders.

No MeSH data available.


Related in: MedlinePlus

Flow cytometric evaluation of the effect of Phyllanthus amarus (100, 200, and 400 mg/kg) on phagocytosis of FITC-conjugated E. coli by neutrophils isolated from treated rats.Abbreviations: Cyclo, cyclophosphamide; FITC, fluorescein isothiocyanate; NSC, nonsensitized control; PA, Phyllanthus amarus; VH, vehicle-treated group; E. coli, Escherichia coli.
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f4-dddt-9-4917: Flow cytometric evaluation of the effect of Phyllanthus amarus (100, 200, and 400 mg/kg) on phagocytosis of FITC-conjugated E. coli by neutrophils isolated from treated rats.Abbreviations: Cyclo, cyclophosphamide; FITC, fluorescein isothiocyanate; NSC, nonsensitized control; PA, Phyllanthus amarus; VH, vehicle-treated group; E. coli, Escherichia coli.

Mentions: Phagocytosis is a process that involves binding and engulfment of invading pathogens by neutrophils. The administration of the extracts of P. amarus to rats resulted in significant (P<0.01) decrease in the engulfment of E. coli by the neutrophils isolated from extract-treated rats in comparison with the control group (Table 1, Figure 4). The results obtained showed that the extract produced a dose-dependent increase in the inhibitory action of phagocytosis, while the highest inhibitory activity (%) was observed at the dose of 400 mg/kg and was comparable to the activity shown by the control used in this study (cyclophosphamide).


Immunosuppressive effects of the standardized extract of Phyllanthus amarus on cellular immune responses in Wistar-Kyoto rats.

Ilangkovan M, Jantan I, Mesaik MA, Bukhari SN - Drug Des Devel Ther (2015)

Flow cytometric evaluation of the effect of Phyllanthus amarus (100, 200, and 400 mg/kg) on phagocytosis of FITC-conjugated E. coli by neutrophils isolated from treated rats.Abbreviations: Cyclo, cyclophosphamide; FITC, fluorescein isothiocyanate; NSC, nonsensitized control; PA, Phyllanthus amarus; VH, vehicle-treated group; E. coli, Escherichia coli.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555964&req=5

f4-dddt-9-4917: Flow cytometric evaluation of the effect of Phyllanthus amarus (100, 200, and 400 mg/kg) on phagocytosis of FITC-conjugated E. coli by neutrophils isolated from treated rats.Abbreviations: Cyclo, cyclophosphamide; FITC, fluorescein isothiocyanate; NSC, nonsensitized control; PA, Phyllanthus amarus; VH, vehicle-treated group; E. coli, Escherichia coli.
Mentions: Phagocytosis is a process that involves binding and engulfment of invading pathogens by neutrophils. The administration of the extracts of P. amarus to rats resulted in significant (P<0.01) decrease in the engulfment of E. coli by the neutrophils isolated from extract-treated rats in comparison with the control group (Table 1, Figure 4). The results obtained showed that the extract produced a dose-dependent increase in the inhibitory action of phagocytosis, while the highest inhibitory activity (%) was observed at the dose of 400 mg/kg and was comparable to the activity shown by the control used in this study (cyclophosphamide).

Bottom Line: The results in P. amarus-treated rats also demonstrated a dose-dependent inhibition of both lipopolysaccharide-stimulated B-cell proliferation and concanavalin A-stimulated T-cell proliferation as compared with sensitized control.At a dose of 400 mg/kg (P<0.01), there was a significant decrease in the (%) expression of CD4(+) and CD8(+) in splenocytes and in serum cytokines of T helper (Th1) (IL-2 and IFN-γ) and Th2 (IL-4).In conclusion, P. amarus showed effective immunosuppressive activities in cellular immune response, by various immune regulatory mechanisms, and may be useful for improvement of immune-related disorders.

View Article: PubMed Central - PubMed

Affiliation: Drug and Herbal Research Center, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.

ABSTRACT
Phyllanthus amarus (family: Euphorbiaceae) is of immense interest due to its wide spectrum of biological activities. In the present study, the standardized 80% ethanol extract of P. amarus was investigated for its modulatory activity on various cellular immune parameters, including chemotaxis of neutrophils, engulfment of Escherichia coli by neutrophils, and Mac-1 expression, in leukocytes isolated from treated/nontreated Wistar-Kyoto rats. The detailed cell-mediated activity of P. amarus was also investigated, including analysis of the effects on T- and B-cell proliferation and CD4(+) and CD8(+) T-cell subsets in splenic mononuclear cells, and estimation of serum cytokine production by activated T-cells. The main components of the extract, phyllanthin, hypophyllanthin, corilagin, geraniin, ellagic acid, and gallic acid were identified and quantitatively analyzed in the extracts, using validated reversed-phase high-performance liquid chromatography (HPLC) methods. N-formyl-methionyl-leucyl-phenylalanine (fMLP)-induced neutrophils isolated from rats administered with the extract of P. amarus, at doses ranging from 100 to 400 mg/kg for 14 days, revealed a significant dose-dependent reduction in neutrophil migration (P<0.05). Similar patterns of inhibition were also observed in phagocytic activity and in fMLP-induced changes in expression of β2 integrin polymorphonuclear neutrophils. The results in P. amarus-treated rats also demonstrated a dose-dependent inhibition of both lipopolysaccharide-stimulated B-cell proliferation and concanavalin A-stimulated T-cell proliferation as compared with sensitized control. At a dose of 400 mg/kg (P<0.01), there was a significant decrease in the (%) expression of CD4(+) and CD8(+) in splenocytes and in serum cytokines of T helper (Th1) (IL-2 and IFN-γ) and Th2 (IL-4). In conclusion, P. amarus showed effective immunosuppressive activities in cellular immune response, by various immune regulatory mechanisms, and may be useful for improvement of immune-related disorders.

No MeSH data available.


Related in: MedlinePlus