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PI(3,5)P2 controls endosomal branched actin dynamics by regulating cortactin-actin interactions.

Hong NH, Qi A, Weaver AM - J. Cell Biol. (2015)

Bottom Line: These findings suggest that PI(3,5)P2 formation on endosomes may remove cortactin from endosome-associated branched actin.Conversely, inhibition of Arp2/3 complex activity greatly reduced cortactin localization to late endosomes.These data suggest a model in which PI(3,5)P2 binding removes cortactin from late endosomal branched actin networks and thereby promotes net actin turnover.

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Affiliation: Department of Cancer Biology, Vanderbilt University Medical Center, Nashville, TN 37232.

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WASH localization is controlled by PI(3,5)P2 levels. (A) Representative images from MDA-MB-231 (top) and SCC61 (bottom) showing localization of WASH (red), cortactin (green), and actin (blue) after 2-h treatment with 800 nM YM201636 or DMSO diluent control. Bars, 20 µm. (B) Representative images of MDA-MB-231 cells stably expressing mRFP-Rab7 (pseudocolored blue) immunostained by WASH (red) and cortactin (green) after 2-h treatment with 800 nM YM201636 or DMS0 diluent control. Bars, 20 µm (5 µm for magnifications). (C and D) Images were analyzed for percentage of colocalization of WASH with Rab7 (C) and Rab7 area/cell (D). 2 independent experiments, n ≥ 62 cells in each condition. ****, P < 0.0001.
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fig8: WASH localization is controlled by PI(3,5)P2 levels. (A) Representative images from MDA-MB-231 (top) and SCC61 (bottom) showing localization of WASH (red), cortactin (green), and actin (blue) after 2-h treatment with 800 nM YM201636 or DMSO diluent control. Bars, 20 µm. (B) Representative images of MDA-MB-231 cells stably expressing mRFP-Rab7 (pseudocolored blue) immunostained by WASH (red) and cortactin (green) after 2-h treatment with 800 nM YM201636 or DMS0 diluent control. Bars, 20 µm (5 µm for magnifications). (C and D) Images were analyzed for percentage of colocalization of WASH with Rab7 (C) and Rab7 area/cell (D). 2 independent experiments, n ≥ 62 cells in each condition. ****, P < 0.0001.

Mentions: Activation of Arp2/3 complex occurs upon binding of an activator in the WASP family of proteins (Millard et al., 2004). At endosomes, the WASP family member WASH appears to be the main activator of Arp2/3 complex (Derivery et al., 2009; Gomez and Billadeau, 2009; Duleh and Welch, 2010) and should act in concert with cortactin to promote endosomal branched actin assembly. Consistent with those studies, WASH, cortactin, and actin show strong colocalization on endosomal membranes (Fig. 8). The FAM21 component of WASH has been found to bind multiple phosphoinositides by dot plot, including the major endosomal lipids PI(3)P and PI(3,5)P2 (Jia et al., 2010). However, the consequence of that interaction is unclear. To assess the effect of PI(3,5)P2 on WASH localization, MDA-MB-231 and SCC61 cells were treated with YM201636 and immunostained for WASH and cortactin and colocalized with either actin (Fig. 8 A) or mRFP-Rab7 (Fig. 8 B). Inhibition of PI(3,5)P2 synthesis led to an increase in the localization of WASH to cortactin+ and actin+ structures at Rab7+ endosomes (Fig. 8), suggesting that similarly to cortactin, PI(3,5)P2 does not recruit WASH to endosomal membranes but instead may promote its removal. However, the increase in WASH localization to Rab7+ endosomes was small compared with the increase in cortactin localization in YM201636-treated cells (40% increase in WASH vs. 300% increase in cortactin colocalization with Rab7, compare median values in Figs. 8 C and 3 C). As with endogenous Rab7 (Fig. 3 E), there was no difference in mRFP-Rab7 area with YM201636 treatment (Fig. 8 D). Overall, PI(3,5)P2 plays a concerted role to remove branched actin regulators from late endosomes and promote disassembly of endosome-associated actin assemblies via cortactin (see model in Fig. 9).


PI(3,5)P2 controls endosomal branched actin dynamics by regulating cortactin-actin interactions.

Hong NH, Qi A, Weaver AM - J. Cell Biol. (2015)

WASH localization is controlled by PI(3,5)P2 levels. (A) Representative images from MDA-MB-231 (top) and SCC61 (bottom) showing localization of WASH (red), cortactin (green), and actin (blue) after 2-h treatment with 800 nM YM201636 or DMSO diluent control. Bars, 20 µm. (B) Representative images of MDA-MB-231 cells stably expressing mRFP-Rab7 (pseudocolored blue) immunostained by WASH (red) and cortactin (green) after 2-h treatment with 800 nM YM201636 or DMS0 diluent control. Bars, 20 µm (5 µm for magnifications). (C and D) Images were analyzed for percentage of colocalization of WASH with Rab7 (C) and Rab7 area/cell (D). 2 independent experiments, n ≥ 62 cells in each condition. ****, P < 0.0001.
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fig8: WASH localization is controlled by PI(3,5)P2 levels. (A) Representative images from MDA-MB-231 (top) and SCC61 (bottom) showing localization of WASH (red), cortactin (green), and actin (blue) after 2-h treatment with 800 nM YM201636 or DMSO diluent control. Bars, 20 µm. (B) Representative images of MDA-MB-231 cells stably expressing mRFP-Rab7 (pseudocolored blue) immunostained by WASH (red) and cortactin (green) after 2-h treatment with 800 nM YM201636 or DMS0 diluent control. Bars, 20 µm (5 µm for magnifications). (C and D) Images were analyzed for percentage of colocalization of WASH with Rab7 (C) and Rab7 area/cell (D). 2 independent experiments, n ≥ 62 cells in each condition. ****, P < 0.0001.
Mentions: Activation of Arp2/3 complex occurs upon binding of an activator in the WASP family of proteins (Millard et al., 2004). At endosomes, the WASP family member WASH appears to be the main activator of Arp2/3 complex (Derivery et al., 2009; Gomez and Billadeau, 2009; Duleh and Welch, 2010) and should act in concert with cortactin to promote endosomal branched actin assembly. Consistent with those studies, WASH, cortactin, and actin show strong colocalization on endosomal membranes (Fig. 8). The FAM21 component of WASH has been found to bind multiple phosphoinositides by dot plot, including the major endosomal lipids PI(3)P and PI(3,5)P2 (Jia et al., 2010). However, the consequence of that interaction is unclear. To assess the effect of PI(3,5)P2 on WASH localization, MDA-MB-231 and SCC61 cells were treated with YM201636 and immunostained for WASH and cortactin and colocalized with either actin (Fig. 8 A) or mRFP-Rab7 (Fig. 8 B). Inhibition of PI(3,5)P2 synthesis led to an increase in the localization of WASH to cortactin+ and actin+ structures at Rab7+ endosomes (Fig. 8), suggesting that similarly to cortactin, PI(3,5)P2 does not recruit WASH to endosomal membranes but instead may promote its removal. However, the increase in WASH localization to Rab7+ endosomes was small compared with the increase in cortactin localization in YM201636-treated cells (40% increase in WASH vs. 300% increase in cortactin colocalization with Rab7, compare median values in Figs. 8 C and 3 C). As with endogenous Rab7 (Fig. 3 E), there was no difference in mRFP-Rab7 area with YM201636 treatment (Fig. 8 D). Overall, PI(3,5)P2 plays a concerted role to remove branched actin regulators from late endosomes and promote disassembly of endosome-associated actin assemblies via cortactin (see model in Fig. 9).

Bottom Line: These findings suggest that PI(3,5)P2 formation on endosomes may remove cortactin from endosome-associated branched actin.Conversely, inhibition of Arp2/3 complex activity greatly reduced cortactin localization to late endosomes.These data suggest a model in which PI(3,5)P2 binding removes cortactin from late endosomal branched actin networks and thereby promotes net actin turnover.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Cancer Biology, Vanderbilt University Medical Center, Nashville, TN 37232.

Show MeSH
Related in: MedlinePlus