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Integrins mediate mechanical compression-induced endothelium-dependent vasodilation through endothelial nitric oxide pathway.

Lu X, Kassab GS - J. Gen. Physiol. (2015)

Bottom Line: We cannulated isolated swine (n = 39) myocardial (n = 69) and skeletal muscle (n = 60) arteriole segments and exposed them to cyclic transmural pressure generated by either intraluminal or extraluminal pressure pulses to simulate compression in contracting muscle.It was attenuated by inhibition of NO synthase and by mechanical removal of the endothelium.We therefore conclude that integrin plays a role in cyclic compression-induced endothelial NO production and thereby in the vasodilation of small arteries during cyclic transmural pressure loading.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biomedical Engineering, Department of Cellular and Integrative Physiology, Department of Surgery, and Indiana Center for Vascular Biology and Medicine, Indiana University-Purdue University Indianapolis, Indianapolis, IN 46202.

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Role of circumferential stretch in the cyclic transmural pressure–induced vasodilation. (A) The representation of the decrease in Pint during BK-induced endothelium-dependent vasodilation. Pext was equal to zero. (B) At isovolumic condition, e.g., approximately constant diameter during cyclic transmural pressure, the cyclic transmural pressure failed to elicit integrin-mediated endothelium-dependent vasodilation in both coronary and skeletal muscular small arterial segments. CAPint, intraluminal pressure of coronary small arterial segment; SMAPint, intraluminal pressure of skeletal muscular small arterial segment.
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fig5: Role of circumferential stretch in the cyclic transmural pressure–induced vasodilation. (A) The representation of the decrease in Pint during BK-induced endothelium-dependent vasodilation. Pext was equal to zero. (B) At isovolumic condition, e.g., approximately constant diameter during cyclic transmural pressure, the cyclic transmural pressure failed to elicit integrin-mediated endothelium-dependent vasodilation in both coronary and skeletal muscular small arterial segments. CAPint, intraluminal pressure of coronary small arterial segment; SMAPint, intraluminal pressure of skeletal muscular small arterial segment.

Mentions: We used isovolumic myography to identify the role of circumferential deformation, as the vasodilation in isovolumic myography is reflected by a decrease in intraluminal pressure at a constant diameter (Fig. 5 A). Isovolumic myography restricts the change of diameter by maintaining constant fluid volume in the vessel segment, and the cyclic transmural pressure can only be generated by extraluminal pulse pressure. We found that the cyclic transmural pressure–induced vasodilation was eliminated under constant diameter condition (Fig. 5 B).


Integrins mediate mechanical compression-induced endothelium-dependent vasodilation through endothelial nitric oxide pathway.

Lu X, Kassab GS - J. Gen. Physiol. (2015)

Role of circumferential stretch in the cyclic transmural pressure–induced vasodilation. (A) The representation of the decrease in Pint during BK-induced endothelium-dependent vasodilation. Pext was equal to zero. (B) At isovolumic condition, e.g., approximately constant diameter during cyclic transmural pressure, the cyclic transmural pressure failed to elicit integrin-mediated endothelium-dependent vasodilation in both coronary and skeletal muscular small arterial segments. CAPint, intraluminal pressure of coronary small arterial segment; SMAPint, intraluminal pressure of skeletal muscular small arterial segment.
© Copyright Policy - openaccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4555471&req=5

fig5: Role of circumferential stretch in the cyclic transmural pressure–induced vasodilation. (A) The representation of the decrease in Pint during BK-induced endothelium-dependent vasodilation. Pext was equal to zero. (B) At isovolumic condition, e.g., approximately constant diameter during cyclic transmural pressure, the cyclic transmural pressure failed to elicit integrin-mediated endothelium-dependent vasodilation in both coronary and skeletal muscular small arterial segments. CAPint, intraluminal pressure of coronary small arterial segment; SMAPint, intraluminal pressure of skeletal muscular small arterial segment.
Mentions: We used isovolumic myography to identify the role of circumferential deformation, as the vasodilation in isovolumic myography is reflected by a decrease in intraluminal pressure at a constant diameter (Fig. 5 A). Isovolumic myography restricts the change of diameter by maintaining constant fluid volume in the vessel segment, and the cyclic transmural pressure can only be generated by extraluminal pulse pressure. We found that the cyclic transmural pressure–induced vasodilation was eliminated under constant diameter condition (Fig. 5 B).

Bottom Line: We cannulated isolated swine (n = 39) myocardial (n = 69) and skeletal muscle (n = 60) arteriole segments and exposed them to cyclic transmural pressure generated by either intraluminal or extraluminal pressure pulses to simulate compression in contracting muscle.It was attenuated by inhibition of NO synthase and by mechanical removal of the endothelium.We therefore conclude that integrin plays a role in cyclic compression-induced endothelial NO production and thereby in the vasodilation of small arteries during cyclic transmural pressure loading.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biomedical Engineering, Department of Cellular and Integrative Physiology, Department of Surgery, and Indiana Center for Vascular Biology and Medicine, Indiana University-Purdue University Indianapolis, Indianapolis, IN 46202.

Show MeSH
Related in: MedlinePlus