Integrins mediate mechanical compression-induced endothelium-dependent vasodilation through endothelial nitric oxide pathway.
Bottom Line: We cannulated isolated swine (n = 39) myocardial (n = 69) and skeletal muscle (n = 60) arteriole segments and exposed them to cyclic transmural pressure generated by either intraluminal or extraluminal pressure pulses to simulate compression in contracting muscle.It was attenuated by inhibition of NO synthase and by mechanical removal of the endothelium.We therefore conclude that integrin plays a role in cyclic compression-induced endothelial NO production and thereby in the vasodilation of small arteries during cyclic transmural pressure loading.
Affiliation: Department of Biomedical Engineering, Department of Cellular and Integrative Physiology, Department of Surgery, and Indiana Center for Vascular Biology and Medicine, Indiana University-Purdue University Indianapolis, Indianapolis, IN 46202.Show MeSH
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Mentions: To assess the role of integrins, we investigated the effect of RGD peptide and the function-blocking integrin α5β1 and αvβ3 antibodies on the cyclic transmural pressure–induced vasodilation. RGD peptides competitively combine with integrin to attenuate mechanical stimuli–induced integrin activation (Mogford et al., 1997; Schlaepfer and Hunter, 1997; Yip and Marsh, 1997; Platts et al., 1998). In Fig. 4 A, using a typical sample of diameter tracings, we show that the preincubation of the vessel segments with RGD peptide inhibited the cyclic transmural pressure–induced vasodilation. GRGESP (a control RGE peptide) did not attenuate the cyclic transmural pressure-induced vasodilation (Fig. 4 B). The diameter tracings of the vessel segments preincubated with the function-blocking integrin α5β1 or αvβ3 antibodies also show that the antibodies attenuated the cyclic transmural pressure–induced vasodilation (Fig. 4 C).
Affiliation: Department of Biomedical Engineering, Department of Cellular and Integrative Physiology, Department of Surgery, and Indiana Center for Vascular Biology and Medicine, Indiana University-Purdue University Indianapolis, Indianapolis, IN 46202.