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Prognostic Significance of mTOR and PTEN in Patients with Esophageal Squamous Cell Carcinoma.

Lu J, Pan Y, Xia X, Gu Y, Lei Y - Biomed Res Int (2015)

Bottom Line: The Pearson correlation coefficient revealed a significant negative correlation in two proteins (correlation coefficient = -0.189, P < 0.005).The difference of survival rate between the two groups remained statistically significant. mTOR-low or PTEN-high patients had better 3-year rates of OS and DFS than mTOR-high or PTEN-low group (P < 0.001 by the log-rank test).This study also found that mTOR was an independence prognostic factor by multivariate analysis.

View Article: PubMed Central - PubMed

Affiliation: Department of Thoracic Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.

ABSTRACT
The prognostic value of mTOR in ESCC is much controversial; this study aimed to determine the prognostic importance of mTOR and PTEN in patients with ESCC. A total of 148 consecutive patients who underwent esophagectomy from 2010 to 2012 were included in this study, tested by western bolt and immunohistochemistry for mTOR and PTEN expression. Correlation coefficient was calculated using Pearson's correlation test. The 3-year overall survival (OS) and disease-free survival (DFS) were calculated in relation to the two markers. 94 (63.5%) of 148 were mTOR high expression, and PTEN high expression was detected in 46 (31.1%) of the 148 patients with ESCC. The Pearson correlation coefficient revealed a significant negative correlation in two proteins (correlation coefficient = -0.189, P < 0.005). The 3-year OS and DFS time in the mTOR-high group was 23.9 and 18.4 months, respectively, and the time in the mTOR-low group was 33.9 months and 31.4 months, respectively. The difference of survival rate between the two groups remained statistically significant. mTOR-low or PTEN-high patients had better 3-year rates of OS and DFS than mTOR-high or PTEN-low group (P < 0.001 by the log-rank test). This study also found that mTOR was an independence prognostic factor by multivariate analysis.

No MeSH data available.


Related in: MedlinePlus

Immunohistochemistry (IHC) analysis of mTOR expression in normal and ESCC tissues. (a) Normal esophageal tissue showed nearly negative expression of mTOR (400x); (b) low expression of mTOR was shown in ESCC patient samples (400x); (c) moderate expression of mTOR was detected in ESCC samples (400x); (d) high expression of mTOR was detected in ESCC samples (400x). ESCC: esophageal squamous cell carcinoma.
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fig2: Immunohistochemistry (IHC) analysis of mTOR expression in normal and ESCC tissues. (a) Normal esophageal tissue showed nearly negative expression of mTOR (400x); (b) low expression of mTOR was shown in ESCC patient samples (400x); (c) moderate expression of mTOR was detected in ESCC samples (400x); (d) high expression of mTOR was detected in ESCC samples (400x). ESCC: esophageal squamous cell carcinoma.

Mentions: The mTOR was detected by western blot and IHC in this study. In the ESCC tumor samples, mTOR protein was elevated by western blot analysis (Figure 1). A total of 148 groups of ESCC tumor sections and 10 tumor-adjacent normal esophageal tissue sections were detected by IHC (Figure 2). In the cohort of 148 ESCC patients, high expression of mTOR was detected in 94 of 148 (63.5%) patients and low expression of mTOR was detected in 54 of 148 (34.5%) patients, including negative expression of mTOR which was detected in 21 of 54 (38.9%). The overall scores of all normal esophageal sections were below 2.


Prognostic Significance of mTOR and PTEN in Patients with Esophageal Squamous Cell Carcinoma.

Lu J, Pan Y, Xia X, Gu Y, Lei Y - Biomed Res Int (2015)

Immunohistochemistry (IHC) analysis of mTOR expression in normal and ESCC tissues. (a) Normal esophageal tissue showed nearly negative expression of mTOR (400x); (b) low expression of mTOR was shown in ESCC patient samples (400x); (c) moderate expression of mTOR was detected in ESCC samples (400x); (d) high expression of mTOR was detected in ESCC samples (400x). ESCC: esophageal squamous cell carcinoma.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555449&req=5

fig2: Immunohistochemistry (IHC) analysis of mTOR expression in normal and ESCC tissues. (a) Normal esophageal tissue showed nearly negative expression of mTOR (400x); (b) low expression of mTOR was shown in ESCC patient samples (400x); (c) moderate expression of mTOR was detected in ESCC samples (400x); (d) high expression of mTOR was detected in ESCC samples (400x). ESCC: esophageal squamous cell carcinoma.
Mentions: The mTOR was detected by western blot and IHC in this study. In the ESCC tumor samples, mTOR protein was elevated by western blot analysis (Figure 1). A total of 148 groups of ESCC tumor sections and 10 tumor-adjacent normal esophageal tissue sections were detected by IHC (Figure 2). In the cohort of 148 ESCC patients, high expression of mTOR was detected in 94 of 148 (63.5%) patients and low expression of mTOR was detected in 54 of 148 (34.5%) patients, including negative expression of mTOR which was detected in 21 of 54 (38.9%). The overall scores of all normal esophageal sections were below 2.

Bottom Line: The Pearson correlation coefficient revealed a significant negative correlation in two proteins (correlation coefficient = -0.189, P < 0.005).The difference of survival rate between the two groups remained statistically significant. mTOR-low or PTEN-high patients had better 3-year rates of OS and DFS than mTOR-high or PTEN-low group (P < 0.001 by the log-rank test).This study also found that mTOR was an independence prognostic factor by multivariate analysis.

View Article: PubMed Central - PubMed

Affiliation: Department of Thoracic Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.

ABSTRACT
The prognostic value of mTOR in ESCC is much controversial; this study aimed to determine the prognostic importance of mTOR and PTEN in patients with ESCC. A total of 148 consecutive patients who underwent esophagectomy from 2010 to 2012 were included in this study, tested by western bolt and immunohistochemistry for mTOR and PTEN expression. Correlation coefficient was calculated using Pearson's correlation test. The 3-year overall survival (OS) and disease-free survival (DFS) were calculated in relation to the two markers. 94 (63.5%) of 148 were mTOR high expression, and PTEN high expression was detected in 46 (31.1%) of the 148 patients with ESCC. The Pearson correlation coefficient revealed a significant negative correlation in two proteins (correlation coefficient = -0.189, P < 0.005). The 3-year OS and DFS time in the mTOR-high group was 23.9 and 18.4 months, respectively, and the time in the mTOR-low group was 33.9 months and 31.4 months, respectively. The difference of survival rate between the two groups remained statistically significant. mTOR-low or PTEN-high patients had better 3-year rates of OS and DFS than mTOR-high or PTEN-low group (P < 0.001 by the log-rank test). This study also found that mTOR was an independence prognostic factor by multivariate analysis.

No MeSH data available.


Related in: MedlinePlus