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Characterization Method for 3D Substructure of Nuclear Cell Based on Orthogonal Phase Images.

Ji Y, Liang M, Hua T, Xu Y, Xin Z, Wang Y - Biomed Res Int (2015)

Bottom Line: A set of optical models associated with blood cells are introduced in this paper.The wrapped phase images as well as the unwrapped ones from two orthogonal directions related to some of these models are obtained by simulation technique.The simulation result shows that characterization with inflexion curve based on orthogonal phase images could describe the substructure of the cells availably, which may provide a new way to identify the typical biological cells quickly without scanning.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Science, Jiangsu University, Zhenjiang 212013, China.

ABSTRACT
A set of optical models associated with blood cells are introduced in this paper. All of these models are made up of different parts possessing symmetries. The wrapped phase images as well as the unwrapped ones from two orthogonal directions related to some of these models are obtained by simulation technique. Because the phase mutation occurs on the boundary between nucleus and cytoplasm as well as on the boundary between cytoplasm and environment medium, the equation of inflexion curve is introduced to describe the size, morphology, and substructure of the nuclear cell based on the analysis of the phase features of the model. Furthermore, a mononuclear cell model is discussed as an example to verify this method. The simulation result shows that characterization with inflexion curve based on orthogonal phase images could describe the substructure of the cells availably, which may provide a new way to identify the typical biological cells quickly without scanning.

No MeSH data available.


Related in: MedlinePlus

The phase maps for two-ellipsoid cell model of nuclear type. (a) The model of the cell of nuclear type. (b) The wrapped phase map on reference plane x-y. (c) The wrapped phase map on reference plane x-z. (d) The unwrapped phase map related to (b).
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fig3: The phase maps for two-ellipsoid cell model of nuclear type. (a) The model of the cell of nuclear type. (b) The wrapped phase map on reference plane x-y. (c) The wrapped phase map on reference plane x-z. (d) The unwrapped phase map related to (b).

Mentions: Here, we take a two-ellipsoid model of nuclear type as an example to explain the reconstruction process. The model is shown in Figure 3(a). Two orthogonal wrapped quantitative phase images of the specimen are presented as Figures 3(b) and 3(c). Combined with the associated unwrapped phase map of the cell shown in Figure 3(d), it is believed that two extreme values existed in the phase distribution.


Characterization Method for 3D Substructure of Nuclear Cell Based on Orthogonal Phase Images.

Ji Y, Liang M, Hua T, Xu Y, Xin Z, Wang Y - Biomed Res Int (2015)

The phase maps for two-ellipsoid cell model of nuclear type. (a) The model of the cell of nuclear type. (b) The wrapped phase map on reference plane x-y. (c) The wrapped phase map on reference plane x-z. (d) The unwrapped phase map related to (b).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555359&req=5

fig3: The phase maps for two-ellipsoid cell model of nuclear type. (a) The model of the cell of nuclear type. (b) The wrapped phase map on reference plane x-y. (c) The wrapped phase map on reference plane x-z. (d) The unwrapped phase map related to (b).
Mentions: Here, we take a two-ellipsoid model of nuclear type as an example to explain the reconstruction process. The model is shown in Figure 3(a). Two orthogonal wrapped quantitative phase images of the specimen are presented as Figures 3(b) and 3(c). Combined with the associated unwrapped phase map of the cell shown in Figure 3(d), it is believed that two extreme values existed in the phase distribution.

Bottom Line: A set of optical models associated with blood cells are introduced in this paper.The wrapped phase images as well as the unwrapped ones from two orthogonal directions related to some of these models are obtained by simulation technique.The simulation result shows that characterization with inflexion curve based on orthogonal phase images could describe the substructure of the cells availably, which may provide a new way to identify the typical biological cells quickly without scanning.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Science, Jiangsu University, Zhenjiang 212013, China.

ABSTRACT
A set of optical models associated with blood cells are introduced in this paper. All of these models are made up of different parts possessing symmetries. The wrapped phase images as well as the unwrapped ones from two orthogonal directions related to some of these models are obtained by simulation technique. Because the phase mutation occurs on the boundary between nucleus and cytoplasm as well as on the boundary between cytoplasm and environment medium, the equation of inflexion curve is introduced to describe the size, morphology, and substructure of the nuclear cell based on the analysis of the phase features of the model. Furthermore, a mononuclear cell model is discussed as an example to verify this method. The simulation result shows that characterization with inflexion curve based on orthogonal phase images could describe the substructure of the cells availably, which may provide a new way to identify the typical biological cells quickly without scanning.

No MeSH data available.


Related in: MedlinePlus