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Biosurfactant Production by Bacillus salmalaya for Lubricating Oil Solubilization and Biodegradation.

Dadrasnia A, Ismail S - Int J Environ Res Public Health (2015)

Bottom Line: The biosurfactant exhibited a high emulsification index and a positive result in the drop collapse test, with the results demonstrating the wetting activity of the biosurfactant and its potential to produce surface-active molecules.Furthermore, the biosurfactant demonstrated high stability at different ranges of salinity, pH, and temperature.Overall, the results indicated the potential use of B. salmalaya 139SI in environmental remediation processes.

View Article: PubMed Central - PubMed

Affiliation: Department of Biohealth Science, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia. are.dadrasnia@gmail.com.

ABSTRACT
This study investigated the capability of a biosurfactant produced by a novel strain of Bacillus salmalaya to enhance the biodegradation rates and bioavailability of organic contaminants. The biosurfactant produced by cultured strain 139SI showed high physicochemical properties and surface activity in the selected medium. The biosurfactant exhibited a high emulsification index and a positive result in the drop collapse test, with the results demonstrating the wetting activity of the biosurfactant and its potential to produce surface-active molecules. Strain 139SI can significantly reduce the surface tension (ST) from 70.5 to 27 mN/m, with a critical micelle concentration of 0.4%. Moreover, lubricating oil at 2% (v/v) was degraded on Day 20 (71.5). Furthermore, the biosurfactant demonstrated high stability at different ranges of salinity, pH, and temperature. Overall, the results indicated the potential use of B. salmalaya 139SI in environmental remediation processes.

No MeSH data available.


Related in: MedlinePlus

Influence of different ranges of (A) pH, (B) temperature and, (C) carbon source on surface tension (ST) reduction.
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ijerph-12-09848-f003: Influence of different ranges of (A) pH, (B) temperature and, (C) carbon source on surface tension (ST) reduction.

Mentions: As many factors influence the effectiveness of biosurfactant activities, these parameters must be examined to confirm the application of a biosurfactant during remediation. In the present study, a wide range of pH and temperature was tested to evaluate the stability of B. salmalaya 139SI to grow and produce biosurfactants. As shown in Figure 3A, a pH of 6.5 was the optimal value for biosurfactant production with the highest rate of ST reduction (38.5 ± 2.1%) when compared with the treatment without inoculation. The results also showed a low level of biosurfactant production at acidic pH (<5) and alkaline pH (>9). Various species produce biosurfactants at different pH values. Bacillus salmalaya 139SI produced the maximum amount of biosurfactant at pH 6.5 when incubated at 36 °C, which was significantly different from the production at other temperatures. The lowest ST was produced at 20 °C (Figure 3B); ST sharply increased when the temperature increased up to 36 °C and then gradually decreased and remained constant at 46 °C. Thus, temperature affects biosurfactant production, a finding that is consistent with the results of other studies [3,25,26,27]. Nonetheless, different Bacillus strains present various optimal temperatures and pH values.


Biosurfactant Production by Bacillus salmalaya for Lubricating Oil Solubilization and Biodegradation.

Dadrasnia A, Ismail S - Int J Environ Res Public Health (2015)

Influence of different ranges of (A) pH, (B) temperature and, (C) carbon source on surface tension (ST) reduction.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555315&req=5

ijerph-12-09848-f003: Influence of different ranges of (A) pH, (B) temperature and, (C) carbon source on surface tension (ST) reduction.
Mentions: As many factors influence the effectiveness of biosurfactant activities, these parameters must be examined to confirm the application of a biosurfactant during remediation. In the present study, a wide range of pH and temperature was tested to evaluate the stability of B. salmalaya 139SI to grow and produce biosurfactants. As shown in Figure 3A, a pH of 6.5 was the optimal value for biosurfactant production with the highest rate of ST reduction (38.5 ± 2.1%) when compared with the treatment without inoculation. The results also showed a low level of biosurfactant production at acidic pH (<5) and alkaline pH (>9). Various species produce biosurfactants at different pH values. Bacillus salmalaya 139SI produced the maximum amount of biosurfactant at pH 6.5 when incubated at 36 °C, which was significantly different from the production at other temperatures. The lowest ST was produced at 20 °C (Figure 3B); ST sharply increased when the temperature increased up to 36 °C and then gradually decreased and remained constant at 46 °C. Thus, temperature affects biosurfactant production, a finding that is consistent with the results of other studies [3,25,26,27]. Nonetheless, different Bacillus strains present various optimal temperatures and pH values.

Bottom Line: The biosurfactant exhibited a high emulsification index and a positive result in the drop collapse test, with the results demonstrating the wetting activity of the biosurfactant and its potential to produce surface-active molecules.Furthermore, the biosurfactant demonstrated high stability at different ranges of salinity, pH, and temperature.Overall, the results indicated the potential use of B. salmalaya 139SI in environmental remediation processes.

View Article: PubMed Central - PubMed

Affiliation: Department of Biohealth Science, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia. are.dadrasnia@gmail.com.

ABSTRACT
This study investigated the capability of a biosurfactant produced by a novel strain of Bacillus salmalaya to enhance the biodegradation rates and bioavailability of organic contaminants. The biosurfactant produced by cultured strain 139SI showed high physicochemical properties and surface activity in the selected medium. The biosurfactant exhibited a high emulsification index and a positive result in the drop collapse test, with the results demonstrating the wetting activity of the biosurfactant and its potential to produce surface-active molecules. Strain 139SI can significantly reduce the surface tension (ST) from 70.5 to 27 mN/m, with a critical micelle concentration of 0.4%. Moreover, lubricating oil at 2% (v/v) was degraded on Day 20 (71.5). Furthermore, the biosurfactant demonstrated high stability at different ranges of salinity, pH, and temperature. Overall, the results indicated the potential use of B. salmalaya 139SI in environmental remediation processes.

No MeSH data available.


Related in: MedlinePlus