Limits...
Tobacco-Specific Nitrosamines in Electronic Cigarettes: Comparison between Liquid and Aerosol Levels.

Farsalinos KE, Gillman G, Poulas K, Voudris V - Int J Environ Res Public Health (2015)

Bottom Line: The spiked sample contained 42.0-53.9 ng/g of each of the TSNAs.All TSNAs were detected in the aerosol with the measured levels being statistically similar to the expected amounts.A significant correlation between expected and measured levels of TSNAs in the aerosol was found (r = 0.83, p < 0.001).

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology, Onassis Cardiac Surgery Center, Sygrou 356, Kallithea 17674, Greece. kfarsalinos@gmail.com.

ABSTRACT

Introduction: Although electronic cigarette (EC) liquids contain low levels of tobacco-specific nitrosamines (TSNAs), studies evaluating the levels emitted to the aerosol are scarce. The purpose of this study was to compare the levels of TSNAs between liquids and generated aerosol.

Methods: Three EC liquids were obtained from the market. An additional (spiked) sample was prepared by adding known amounts of standard TSNAs solutions to one of the obtained liquids. N-nitrosonornicotine (NNN), N-nitrosoanatabine (NAT), N-nitrosoanabasine (NAB) and 4-(methylnitrosamino)1-(3-pyridyl)-1-butanone (NNK) were measured. Three 100-puff sets from each liquid were trapped in filter pads and were subsequently analyzed for the presence of TSNAs. The expected levels of TSNAs (calculated based on the liquid consumption) were compared with the measured levels in the aerosol.

Results: Only NAB was found at trace levels in two commercial liquids (1.2 and 2.3 ng/g), while the third contained 1.5 ng/g NAB and 7.7 ng/g NNN. The 100-puff sets resulted in 336-515 mg liquid consumption, with no TSNAs being detected in the aerosol. The spiked sample contained 42.0-53.9 ng/g of each of the TSNAs. All TSNAs were detected in the aerosol with the measured levels being statistically similar to the expected amounts. A significant correlation between expected and measured levels of TSNAs in the aerosol was found (r = 0.83, p < 0.001).

Conclusion: The findings of this study show that exposure of EC users to TSNAs can be accurately assessed based on the levels present in the liquid, without the need to analyze the aerosol.

No MeSH data available.


Related in: MedlinePlus

Expected and measured levels of tobacco-specific nitrosamines (TSNAs) in the electronic cigarette aerosol generated from the spiked liquid sample. Bars represent the average from three sets of 100 puffs each while error bars represent standard deviation. For abbreviations, see Table 1.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4555263&req=5

ijerph-12-09046-f001: Expected and measured levels of tobacco-specific nitrosamines (TSNAs) in the electronic cigarette aerosol generated from the spiked liquid sample. Bars represent the average from three sets of 100 puffs each while error bars represent standard deviation. For abbreviations, see Table 1.

Mentions: The 100-puff sets of commercial liquids resulted in the average liquid consumption ranging from 336 to 515 mg for each of the liquid. Due to the very low levels of TSNAs in the commercial liquids, the respective aerosol samples contained, as expected, no TSNAs above the LOD. The analysis of the aerosol generated from the spiked sample is shown in Figure 1.


Tobacco-Specific Nitrosamines in Electronic Cigarettes: Comparison between Liquid and Aerosol Levels.

Farsalinos KE, Gillman G, Poulas K, Voudris V - Int J Environ Res Public Health (2015)

Expected and measured levels of tobacco-specific nitrosamines (TSNAs) in the electronic cigarette aerosol generated from the spiked liquid sample. Bars represent the average from three sets of 100 puffs each while error bars represent standard deviation. For abbreviations, see Table 1.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555263&req=5

ijerph-12-09046-f001: Expected and measured levels of tobacco-specific nitrosamines (TSNAs) in the electronic cigarette aerosol generated from the spiked liquid sample. Bars represent the average from three sets of 100 puffs each while error bars represent standard deviation. For abbreviations, see Table 1.
Mentions: The 100-puff sets of commercial liquids resulted in the average liquid consumption ranging from 336 to 515 mg for each of the liquid. Due to the very low levels of TSNAs in the commercial liquids, the respective aerosol samples contained, as expected, no TSNAs above the LOD. The analysis of the aerosol generated from the spiked sample is shown in Figure 1.

Bottom Line: The spiked sample contained 42.0-53.9 ng/g of each of the TSNAs.All TSNAs were detected in the aerosol with the measured levels being statistically similar to the expected amounts.A significant correlation between expected and measured levels of TSNAs in the aerosol was found (r = 0.83, p < 0.001).

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology, Onassis Cardiac Surgery Center, Sygrou 356, Kallithea 17674, Greece. kfarsalinos@gmail.com.

ABSTRACT

Introduction: Although electronic cigarette (EC) liquids contain low levels of tobacco-specific nitrosamines (TSNAs), studies evaluating the levels emitted to the aerosol are scarce. The purpose of this study was to compare the levels of TSNAs between liquids and generated aerosol.

Methods: Three EC liquids were obtained from the market. An additional (spiked) sample was prepared by adding known amounts of standard TSNAs solutions to one of the obtained liquids. N-nitrosonornicotine (NNN), N-nitrosoanatabine (NAT), N-nitrosoanabasine (NAB) and 4-(methylnitrosamino)1-(3-pyridyl)-1-butanone (NNK) were measured. Three 100-puff sets from each liquid were trapped in filter pads and were subsequently analyzed for the presence of TSNAs. The expected levels of TSNAs (calculated based on the liquid consumption) were compared with the measured levels in the aerosol.

Results: Only NAB was found at trace levels in two commercial liquids (1.2 and 2.3 ng/g), while the third contained 1.5 ng/g NAB and 7.7 ng/g NNN. The 100-puff sets resulted in 336-515 mg liquid consumption, with no TSNAs being detected in the aerosol. The spiked sample contained 42.0-53.9 ng/g of each of the TSNAs. All TSNAs were detected in the aerosol with the measured levels being statistically similar to the expected amounts. A significant correlation between expected and measured levels of TSNAs in the aerosol was found (r = 0.83, p < 0.001).

Conclusion: The findings of this study show that exposure of EC users to TSNAs can be accurately assessed based on the levels present in the liquid, without the need to analyze the aerosol.

No MeSH data available.


Related in: MedlinePlus