Limits...
Chronic Exposure to Static Magnetic Fields from Magnetic Resonance Imaging Devices Deserves Screening for Osteoporosis and Vitamin D Levels: A Rat Model.

Gungor HR, Akkaya S, Ok N, Yorukoglu A, Yorukoglu C, Kiter E, Oguz EO, Keskin N, Mete GA - Int J Environ Res Public Health (2015)

Bottom Line: The mean vitamin D level in Group A was lower than in the other groups (p = 0.002).The mean cortical thickness, the mean trabecular wall thickness, and number of trabeculae per 1 mm2 were significantly lower in Group A (p = 0.003).TUNEL assay revealed that apoptosis of osteocytes were significantly greater in Group A than the other groups (p = 0.005).

View Article: PubMed Central - PubMed

Affiliation: Orthopedics and Traumatology Department, Pamukkale University Medical Faculty, Denizli 20070, Turkey. hrgungor@gmail.com.

ABSTRACT
Technicians often receive chronic magnetic exposures from magnetic resonance imaging (MRI) devices, mainly due to static magnetic fields (SMFs). Here, we ascertain the biological effects of chronic exposure to SMFs from MRI devices on the bone quality using rats exposed to SMFs in MRI examining rooms. Eighteen Wistar albino male rats were randomly assigned to SMF exposure (A), sham (B), and control (C) groups. Group A rats were positioned within 50 centimeters of the bore of the magnet of 1.5 T MRI machine during the nighttime for 8 weeks. We collected blood samples for biochemical analysis, and bone tissue samples for electron microscopic and histological analysis. The mean vitamin D level in Group A was lower than in the other groups (p = 0.002). The mean cortical thickness, the mean trabecular wall thickness, and number of trabeculae per 1 mm2 were significantly lower in Group A (p = 0.003). TUNEL assay revealed that apoptosis of osteocytes were significantly greater in Group A than the other groups (p = 0.005). The effect of SMFs in chronic exposure is related to movement within the magnetic field that induces low-frequency fields within the tissues. These fields can exceed the exposure limits necessary to deteriorate bone microstructure and vitamin D metabolism.

No MeSH data available.


Related in: MedlinePlus

TEM evaluation of Group A (a) Heterochromatic osteocyte (black arrow), decreased and condensed cytoplasm with round appearances (white arrow; at 26K×); (b) Round appearances within cytoplasm (white arrow), mitochondria (M) and nucleus (N; at 56K×).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4555256&req=5

ijerph-12-08919-f005: TEM evaluation of Group A (a) Heterochromatic osteocyte (black arrow), decreased and condensed cytoplasm with round appearances (white arrow; at 26K×); (b) Round appearances within cytoplasm (white arrow), mitochondria (M) and nucleus (N; at 56K×).

Mentions: In TEM evaluation, the osteocytes were settled in bone lacunas. The cytoplasmic extensions protruding into the neighboring cells were evident in Group C, and a large number of round mitochondria were observed in cytoplasm. The chromatin distribution in the nucleus was also in adequate form in Group C (Figure 3). In Group B, it was observed that the osteocytes were narrowed compared to those in the control group. The cumulation in amorphous fashion in cytoplasm was distinctive. There was degeneration and crystallization in some mitochondria. GER and Golgi were in their normal appearance. An increase in heterochromatic areas of the nucleus was observed (Figure 4). The osteocytes in Group A were smaller than those in the control group. The observed cytoplasm was decreased, and the cytoplasmic extensions to the neighboring cells were disappeared in Group A. Interestingly, the structures in round appearances that are most probably mitochondria were observed in largely disordered and scattered cytoplasm. It was also observed that the chromatin distribution in the nucleus was changed and had a heterochromatic appearance (Figure 5).


Chronic Exposure to Static Magnetic Fields from Magnetic Resonance Imaging Devices Deserves Screening for Osteoporosis and Vitamin D Levels: A Rat Model.

Gungor HR, Akkaya S, Ok N, Yorukoglu A, Yorukoglu C, Kiter E, Oguz EO, Keskin N, Mete GA - Int J Environ Res Public Health (2015)

TEM evaluation of Group A (a) Heterochromatic osteocyte (black arrow), decreased and condensed cytoplasm with round appearances (white arrow; at 26K×); (b) Round appearances within cytoplasm (white arrow), mitochondria (M) and nucleus (N; at 56K×).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555256&req=5

ijerph-12-08919-f005: TEM evaluation of Group A (a) Heterochromatic osteocyte (black arrow), decreased and condensed cytoplasm with round appearances (white arrow; at 26K×); (b) Round appearances within cytoplasm (white arrow), mitochondria (M) and nucleus (N; at 56K×).
Mentions: In TEM evaluation, the osteocytes were settled in bone lacunas. The cytoplasmic extensions protruding into the neighboring cells were evident in Group C, and a large number of round mitochondria were observed in cytoplasm. The chromatin distribution in the nucleus was also in adequate form in Group C (Figure 3). In Group B, it was observed that the osteocytes were narrowed compared to those in the control group. The cumulation in amorphous fashion in cytoplasm was distinctive. There was degeneration and crystallization in some mitochondria. GER and Golgi were in their normal appearance. An increase in heterochromatic areas of the nucleus was observed (Figure 4). The osteocytes in Group A were smaller than those in the control group. The observed cytoplasm was decreased, and the cytoplasmic extensions to the neighboring cells were disappeared in Group A. Interestingly, the structures in round appearances that are most probably mitochondria were observed in largely disordered and scattered cytoplasm. It was also observed that the chromatin distribution in the nucleus was changed and had a heterochromatic appearance (Figure 5).

Bottom Line: The mean vitamin D level in Group A was lower than in the other groups (p = 0.002).The mean cortical thickness, the mean trabecular wall thickness, and number of trabeculae per 1 mm2 were significantly lower in Group A (p = 0.003).TUNEL assay revealed that apoptosis of osteocytes were significantly greater in Group A than the other groups (p = 0.005).

View Article: PubMed Central - PubMed

Affiliation: Orthopedics and Traumatology Department, Pamukkale University Medical Faculty, Denizli 20070, Turkey. hrgungor@gmail.com.

ABSTRACT
Technicians often receive chronic magnetic exposures from magnetic resonance imaging (MRI) devices, mainly due to static magnetic fields (SMFs). Here, we ascertain the biological effects of chronic exposure to SMFs from MRI devices on the bone quality using rats exposed to SMFs in MRI examining rooms. Eighteen Wistar albino male rats were randomly assigned to SMF exposure (A), sham (B), and control (C) groups. Group A rats were positioned within 50 centimeters of the bore of the magnet of 1.5 T MRI machine during the nighttime for 8 weeks. We collected blood samples for biochemical analysis, and bone tissue samples for electron microscopic and histological analysis. The mean vitamin D level in Group A was lower than in the other groups (p = 0.002). The mean cortical thickness, the mean trabecular wall thickness, and number of trabeculae per 1 mm2 were significantly lower in Group A (p = 0.003). TUNEL assay revealed that apoptosis of osteocytes were significantly greater in Group A than the other groups (p = 0.005). The effect of SMFs in chronic exposure is related to movement within the magnetic field that induces low-frequency fields within the tissues. These fields can exceed the exposure limits necessary to deteriorate bone microstructure and vitamin D metabolism.

No MeSH data available.


Related in: MedlinePlus