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Enrichment of H3K9me2 on Unsynapsed Chromatin in Caenorhabditis elegans Does Not Target de Novo Sites.

Guo Y, Yang B, Li Y, Xu X, Maine EM - G3 (Bethesda) (2015)

Bottom Line: Loss of the SET domain protein, MET-2, greatly reduces H3K9me2 abundance and results in germline mortality.These results suggest that MET-2 activity is elevated in him-8 mutants generally as well as targeted preferentially to the unsynapsed X.We hypothesize H3K9me2 may have a structural function critical for germline immortality, and a greater abundance of these marks may be required when a chromosome does not synapse.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Syracuse University, Syracuse, New York 13244.

No MeSH data available.


Distribution of histone H3 lysine 9 dimethylation (H3K9me2) chromatin immunoprecipitation sequencing reads relative to genome features. Pie charts indicate the percentages of H3K9me2-enriched regions that are located in the (predicted) promoter regions, 5′ untranslated regions (UTRs), coding exons, introns, 3′ UTRs, and intergenic regions. We note that promoter and 5′ UTR regions are as predicted in the current C. elegans genome database (WS 220) and in many cases are only approximate. Input is included for comparison. Overall, H3K9me2 is enriched in introns and depleted in exons with respect to input; other regions are similar to input. Each category is mutually exclusive.
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fig2: Distribution of histone H3 lysine 9 dimethylation (H3K9me2) chromatin immunoprecipitation sequencing reads relative to genome features. Pie charts indicate the percentages of H3K9me2-enriched regions that are located in the (predicted) promoter regions, 5′ untranslated regions (UTRs), coding exons, introns, 3′ UTRs, and intergenic regions. We note that promoter and 5′ UTR regions are as predicted in the current C. elegans genome database (WS 220) and in many cases are only approximate. Input is included for comparison. Overall, H3K9me2 is enriched in introns and depleted in exons with respect to input; other regions are similar to input. Each category is mutually exclusive.

Mentions: Analysis of H3K9me2 distribution relative to gene features indicates overall enrichment in introns and depletion in exons, reflecting the presence of interspersed repeats in many introns and their absence from exons (Figure 2 and Figure S2). H3K9me2-enriched regions are underrepresented on the X chromosome relative to autosomes, as illustrated in Figure 3 where the proportion of total H3K9me2-enriched sequence in the genome is represented on a per chromosome basis (see the section Materials and Methods). This enrichment pattern may reflect (at least in part) the relative paucity of repetitive sequences on the X: 12.9% of X chromosomal sequences are repetitive compared with 18.9–24% of autosomal sequences (data from wormbase.org).


Enrichment of H3K9me2 on Unsynapsed Chromatin in Caenorhabditis elegans Does Not Target de Novo Sites.

Guo Y, Yang B, Li Y, Xu X, Maine EM - G3 (Bethesda) (2015)

Distribution of histone H3 lysine 9 dimethylation (H3K9me2) chromatin immunoprecipitation sequencing reads relative to genome features. Pie charts indicate the percentages of H3K9me2-enriched regions that are located in the (predicted) promoter regions, 5′ untranslated regions (UTRs), coding exons, introns, 3′ UTRs, and intergenic regions. We note that promoter and 5′ UTR regions are as predicted in the current C. elegans genome database (WS 220) and in many cases are only approximate. Input is included for comparison. Overall, H3K9me2 is enriched in introns and depleted in exons with respect to input; other regions are similar to input. Each category is mutually exclusive.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555223&req=5

fig2: Distribution of histone H3 lysine 9 dimethylation (H3K9me2) chromatin immunoprecipitation sequencing reads relative to genome features. Pie charts indicate the percentages of H3K9me2-enriched regions that are located in the (predicted) promoter regions, 5′ untranslated regions (UTRs), coding exons, introns, 3′ UTRs, and intergenic regions. We note that promoter and 5′ UTR regions are as predicted in the current C. elegans genome database (WS 220) and in many cases are only approximate. Input is included for comparison. Overall, H3K9me2 is enriched in introns and depleted in exons with respect to input; other regions are similar to input. Each category is mutually exclusive.
Mentions: Analysis of H3K9me2 distribution relative to gene features indicates overall enrichment in introns and depletion in exons, reflecting the presence of interspersed repeats in many introns and their absence from exons (Figure 2 and Figure S2). H3K9me2-enriched regions are underrepresented on the X chromosome relative to autosomes, as illustrated in Figure 3 where the proportion of total H3K9me2-enriched sequence in the genome is represented on a per chromosome basis (see the section Materials and Methods). This enrichment pattern may reflect (at least in part) the relative paucity of repetitive sequences on the X: 12.9% of X chromosomal sequences are repetitive compared with 18.9–24% of autosomal sequences (data from wormbase.org).

Bottom Line: Loss of the SET domain protein, MET-2, greatly reduces H3K9me2 abundance and results in germline mortality.These results suggest that MET-2 activity is elevated in him-8 mutants generally as well as targeted preferentially to the unsynapsed X.We hypothesize H3K9me2 may have a structural function critical for germline immortality, and a greater abundance of these marks may be required when a chromosome does not synapse.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Syracuse University, Syracuse, New York 13244.

No MeSH data available.