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Transcriptomic Analysis of Musca domestica to Reveal Key Genes of the Prophenoloxidase-Activating System.

Li D, Liang Y, Wang X, Wang L, Qi M, Yu Y, Luan Y - G3 (Bethesda) (2015)

Bottom Line: Of the 89,842 unigenes, based on a similarity search with known genes in other insects, 24 putative genes related to the proPO system were identified.Eight of the identified genes encoded for peptidoglycan recognition receptors, two encoded for prophenoloxidases, three encoded for prophenoloxidase-activating enzymes, and 11 encoded for serine proteinase inhibitors.Collectively, this study has provided the comprehensive transcriptomic data of an insect and some fundamental basis toward achieving understanding of the activation mechanisms and immune functions of the proPO system in M. domestica.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Department, The School of Biological Sciences and Biotechnology, University of Jinan, Jinan 250022, People's Republic of China chm_lidx@ujn.edu.cn.

No MeSH data available.


Related in: MedlinePlus

The interspecific phylogenetic analyses of mdSerpin unigenes. The amino acid sequences from 22 mdSerpin unigenes in M. domestica and seven Dm-Sps (Dm-Sp1 to Dm-Sp7: FBpp008812, FBpp0079094, FBpp0079171, FBpp0079243, FBpp0080979, FBpp0289586, FBpp0110138) of Drosophila melanogaster and 18 Rm-Sps (Rm-Sp1 to Rm-Sp18: AHC98652.1, AHC98653.1, AHC98654.1, AHC98655.1, AHC98656.1, AHC98657.1, AHC98658.1, AHC98659.1, AHC98660.1, AHC98661.1, AHC98662.1, AHC98663.1, AHC98664.1, AHC98665.1, AHC98666.1, AHC98667.1, AHC98668.1, AHC98669.1) of Rhipicephalus microplus were used to build the NJ phylogenetic tree by MEGA 5.0 with 1000 bootstraps. Eleven representative unigenes (triangle) were clustered into 11 groups together with known genes of D. melanogaster and R. microplus.
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fig8: The interspecific phylogenetic analyses of mdSerpin unigenes. The amino acid sequences from 22 mdSerpin unigenes in M. domestica and seven Dm-Sps (Dm-Sp1 to Dm-Sp7: FBpp008812, FBpp0079094, FBpp0079171, FBpp0079243, FBpp0080979, FBpp0289586, FBpp0110138) of Drosophila melanogaster and 18 Rm-Sps (Rm-Sp1 to Rm-Sp18: AHC98652.1, AHC98653.1, AHC98654.1, AHC98655.1, AHC98656.1, AHC98657.1, AHC98658.1, AHC98659.1, AHC98660.1, AHC98661.1, AHC98662.1, AHC98663.1, AHC98664.1, AHC98665.1, AHC98666.1, AHC98667.1, AHC98668.1, AHC98669.1) of Rhipicephalus microplus were used to build the NJ phylogenetic tree by MEGA 5.0 with 1000 bootstraps. Eleven representative unigenes (triangle) were clustered into 11 groups together with known genes of D. melanogaster and R. microplus.

Mentions: The putative genes were provided mainly with reference to unigene annotation and unigene phylogenetic analysis (see Figure 5, Figure 6, Figure 7, Figure 8, and Figure S1).


Transcriptomic Analysis of Musca domestica to Reveal Key Genes of the Prophenoloxidase-Activating System.

Li D, Liang Y, Wang X, Wang L, Qi M, Yu Y, Luan Y - G3 (Bethesda) (2015)

The interspecific phylogenetic analyses of mdSerpin unigenes. The amino acid sequences from 22 mdSerpin unigenes in M. domestica and seven Dm-Sps (Dm-Sp1 to Dm-Sp7: FBpp008812, FBpp0079094, FBpp0079171, FBpp0079243, FBpp0080979, FBpp0289586, FBpp0110138) of Drosophila melanogaster and 18 Rm-Sps (Rm-Sp1 to Rm-Sp18: AHC98652.1, AHC98653.1, AHC98654.1, AHC98655.1, AHC98656.1, AHC98657.1, AHC98658.1, AHC98659.1, AHC98660.1, AHC98661.1, AHC98662.1, AHC98663.1, AHC98664.1, AHC98665.1, AHC98666.1, AHC98667.1, AHC98668.1, AHC98669.1) of Rhipicephalus microplus were used to build the NJ phylogenetic tree by MEGA 5.0 with 1000 bootstraps. Eleven representative unigenes (triangle) were clustered into 11 groups together with known genes of D. melanogaster and R. microplus.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555219&req=5

fig8: The interspecific phylogenetic analyses of mdSerpin unigenes. The amino acid sequences from 22 mdSerpin unigenes in M. domestica and seven Dm-Sps (Dm-Sp1 to Dm-Sp7: FBpp008812, FBpp0079094, FBpp0079171, FBpp0079243, FBpp0080979, FBpp0289586, FBpp0110138) of Drosophila melanogaster and 18 Rm-Sps (Rm-Sp1 to Rm-Sp18: AHC98652.1, AHC98653.1, AHC98654.1, AHC98655.1, AHC98656.1, AHC98657.1, AHC98658.1, AHC98659.1, AHC98660.1, AHC98661.1, AHC98662.1, AHC98663.1, AHC98664.1, AHC98665.1, AHC98666.1, AHC98667.1, AHC98668.1, AHC98669.1) of Rhipicephalus microplus were used to build the NJ phylogenetic tree by MEGA 5.0 with 1000 bootstraps. Eleven representative unigenes (triangle) were clustered into 11 groups together with known genes of D. melanogaster and R. microplus.
Mentions: The putative genes were provided mainly with reference to unigene annotation and unigene phylogenetic analysis (see Figure 5, Figure 6, Figure 7, Figure 8, and Figure S1).

Bottom Line: Of the 89,842 unigenes, based on a similarity search with known genes in other insects, 24 putative genes related to the proPO system were identified.Eight of the identified genes encoded for peptidoglycan recognition receptors, two encoded for prophenoloxidases, three encoded for prophenoloxidase-activating enzymes, and 11 encoded for serine proteinase inhibitors.Collectively, this study has provided the comprehensive transcriptomic data of an insect and some fundamental basis toward achieving understanding of the activation mechanisms and immune functions of the proPO system in M. domestica.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Department, The School of Biological Sciences and Biotechnology, University of Jinan, Jinan 250022, People's Republic of China chm_lidx@ujn.edu.cn.

No MeSH data available.


Related in: MedlinePlus