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Transcriptomic Analysis of Musca domestica to Reveal Key Genes of the Prophenoloxidase-Activating System.

Li D, Liang Y, Wang X, Wang L, Qi M, Yu Y, Luan Y - G3 (Bethesda) (2015)

Bottom Line: Of the 89,842 unigenes, based on a similarity search with known genes in other insects, 24 putative genes related to the proPO system were identified.Eight of the identified genes encoded for peptidoglycan recognition receptors, two encoded for prophenoloxidases, three encoded for prophenoloxidase-activating enzymes, and 11 encoded for serine proteinase inhibitors.Collectively, this study has provided the comprehensive transcriptomic data of an insect and some fundamental basis toward achieving understanding of the activation mechanisms and immune functions of the proPO system in M. domestica.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Department, The School of Biological Sciences and Biotechnology, University of Jinan, Jinan 250022, People's Republic of China chm_lidx@ujn.edu.cn.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic analysis of mdPAP unigenes. The amino acid sequences from 12 mdPAP unigenes in M. domestica and 12 known genes of prophenoloxidase activating factor in other species including Drosophila melanogaster (Dm-easter AAF55170; Dm-MP1 AAF52151.3; Dm-MP2 AAF54143.1), Holotrichia diomphalia (Hd-PPAE I precursor BAA34642.1; Hd-PPAF III BAC15604.1), Tenebrio molitor (Tm-SPE BAG14262.2), Bombyx mori (Bm-BAEE NP_001036844.1; Bm-PPAE NP_001036832.1), Manduca sexta (Ms-PAP1 AAX18636.1; Ms-PAP2 AAL76085.1; Ms-PAP3 AAO74570.1), and Tachypleus tridentatus (Tt-PCE AAA30094.1) were used to build the NJ phylogenetic tree by MEGA 5.0 with 1000 bootstraps. The circle-marked five unigenes and Dm-easter and Dm-MP1 were clustered into group 1. Two unigenes and one Dm-MP2 (diamond) were divided to group 2. The other five unigenes and Tt-PCE (triangle) were clustered into group 3.
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fig7: Phylogenetic analysis of mdPAP unigenes. The amino acid sequences from 12 mdPAP unigenes in M. domestica and 12 known genes of prophenoloxidase activating factor in other species including Drosophila melanogaster (Dm-easter AAF55170; Dm-MP1 AAF52151.3; Dm-MP2 AAF54143.1), Holotrichia diomphalia (Hd-PPAE I precursor BAA34642.1; Hd-PPAF III BAC15604.1), Tenebrio molitor (Tm-SPE BAG14262.2), Bombyx mori (Bm-BAEE NP_001036844.1; Bm-PPAE NP_001036832.1), Manduca sexta (Ms-PAP1 AAX18636.1; Ms-PAP2 AAL76085.1; Ms-PAP3 AAO74570.1), and Tachypleus tridentatus (Tt-PCE AAA30094.1) were used to build the NJ phylogenetic tree by MEGA 5.0 with 1000 bootstraps. The circle-marked five unigenes and Dm-easter and Dm-MP1 were clustered into group 1. Two unigenes and one Dm-MP2 (diamond) were divided to group 2. The other five unigenes and Tt-PCE (triangle) were clustered into group 3.

Mentions: The putative genes were provided mainly with reference to unigene annotation and unigene phylogenetic analysis (see Figure 5, Figure 6, Figure 7, Figure 8, and Figure S1).


Transcriptomic Analysis of Musca domestica to Reveal Key Genes of the Prophenoloxidase-Activating System.

Li D, Liang Y, Wang X, Wang L, Qi M, Yu Y, Luan Y - G3 (Bethesda) (2015)

Phylogenetic analysis of mdPAP unigenes. The amino acid sequences from 12 mdPAP unigenes in M. domestica and 12 known genes of prophenoloxidase activating factor in other species including Drosophila melanogaster (Dm-easter AAF55170; Dm-MP1 AAF52151.3; Dm-MP2 AAF54143.1), Holotrichia diomphalia (Hd-PPAE I precursor BAA34642.1; Hd-PPAF III BAC15604.1), Tenebrio molitor (Tm-SPE BAG14262.2), Bombyx mori (Bm-BAEE NP_001036844.1; Bm-PPAE NP_001036832.1), Manduca sexta (Ms-PAP1 AAX18636.1; Ms-PAP2 AAL76085.1; Ms-PAP3 AAO74570.1), and Tachypleus tridentatus (Tt-PCE AAA30094.1) were used to build the NJ phylogenetic tree by MEGA 5.0 with 1000 bootstraps. The circle-marked five unigenes and Dm-easter and Dm-MP1 were clustered into group 1. Two unigenes and one Dm-MP2 (diamond) were divided to group 2. The other five unigenes and Tt-PCE (triangle) were clustered into group 3.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555219&req=5

fig7: Phylogenetic analysis of mdPAP unigenes. The amino acid sequences from 12 mdPAP unigenes in M. domestica and 12 known genes of prophenoloxidase activating factor in other species including Drosophila melanogaster (Dm-easter AAF55170; Dm-MP1 AAF52151.3; Dm-MP2 AAF54143.1), Holotrichia diomphalia (Hd-PPAE I precursor BAA34642.1; Hd-PPAF III BAC15604.1), Tenebrio molitor (Tm-SPE BAG14262.2), Bombyx mori (Bm-BAEE NP_001036844.1; Bm-PPAE NP_001036832.1), Manduca sexta (Ms-PAP1 AAX18636.1; Ms-PAP2 AAL76085.1; Ms-PAP3 AAO74570.1), and Tachypleus tridentatus (Tt-PCE AAA30094.1) were used to build the NJ phylogenetic tree by MEGA 5.0 with 1000 bootstraps. The circle-marked five unigenes and Dm-easter and Dm-MP1 were clustered into group 1. Two unigenes and one Dm-MP2 (diamond) were divided to group 2. The other five unigenes and Tt-PCE (triangle) were clustered into group 3.
Mentions: The putative genes were provided mainly with reference to unigene annotation and unigene phylogenetic analysis (see Figure 5, Figure 6, Figure 7, Figure 8, and Figure S1).

Bottom Line: Of the 89,842 unigenes, based on a similarity search with known genes in other insects, 24 putative genes related to the proPO system were identified.Eight of the identified genes encoded for peptidoglycan recognition receptors, two encoded for prophenoloxidases, three encoded for prophenoloxidase-activating enzymes, and 11 encoded for serine proteinase inhibitors.Collectively, this study has provided the comprehensive transcriptomic data of an insect and some fundamental basis toward achieving understanding of the activation mechanisms and immune functions of the proPO system in M. domestica.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Department, The School of Biological Sciences and Biotechnology, University of Jinan, Jinan 250022, People's Republic of China chm_lidx@ujn.edu.cn.

No MeSH data available.


Related in: MedlinePlus