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Transcriptomic Analysis of Musca domestica to Reveal Key Genes of the Prophenoloxidase-Activating System.

Li D, Liang Y, Wang X, Wang L, Qi M, Yu Y, Luan Y - G3 (Bethesda) (2015)

Bottom Line: Of the 89,842 unigenes, based on a similarity search with known genes in other insects, 24 putative genes related to the proPO system were identified.Eight of the identified genes encoded for peptidoglycan recognition receptors, two encoded for prophenoloxidases, three encoded for prophenoloxidase-activating enzymes, and 11 encoded for serine proteinase inhibitors.Collectively, this study has provided the comprehensive transcriptomic data of an insect and some fundamental basis toward achieving understanding of the activation mechanisms and immune functions of the proPO system in M. domestica.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Department, The School of Biological Sciences and Biotechnology, University of Jinan, Jinan 250022, People's Republic of China chm_lidx@ujn.edu.cn.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic analysis of mdproPO unigenes. The amino acid sequences from eight unigenes of mdproPO in M. domestica and 21 proPO genes in other species including M. domestica (Md, 1), Sarcophaga bullata (Sb, 2), Drosophila melanogaster (Dm, 3), Anopheles gambiae (Ag, 2), Anopheles stephensi (As,1), Armigeres subalbatus (Ars, 3), Aedes aegypti (Aa, 1), Manduca sexta (Ms, 2), Bombyx mori (Bm, 2), Tenebrio molitor (Tm, 1), Holotrichia diomphalia (Hd, 2), and Eriocheir sinensis (Es, 1) were used to build the NJ phylogenetic tree by MEGA 5.0 with 1000 bootstraps. Six unigenes (circle) were clustered with Sb-proPO1 (disc) into group 1, and the other two unigenes (triangles) were clustered with Sb-proPO2 (triangle block) into group 2.
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fig6: Phylogenetic analysis of mdproPO unigenes. The amino acid sequences from eight unigenes of mdproPO in M. domestica and 21 proPO genes in other species including M. domestica (Md, 1), Sarcophaga bullata (Sb, 2), Drosophila melanogaster (Dm, 3), Anopheles gambiae (Ag, 2), Anopheles stephensi (As,1), Armigeres subalbatus (Ars, 3), Aedes aegypti (Aa, 1), Manduca sexta (Ms, 2), Bombyx mori (Bm, 2), Tenebrio molitor (Tm, 1), Holotrichia diomphalia (Hd, 2), and Eriocheir sinensis (Es, 1) were used to build the NJ phylogenetic tree by MEGA 5.0 with 1000 bootstraps. Six unigenes (circle) were clustered with Sb-proPO1 (disc) into group 1, and the other two unigenes (triangles) were clustered with Sb-proPO2 (triangle block) into group 2.

Mentions: The putative genes were provided mainly with reference to unigene annotation and unigene phylogenetic analysis (see Figure 5, Figure 6, Figure 7, Figure 8, and Figure S1).


Transcriptomic Analysis of Musca domestica to Reveal Key Genes of the Prophenoloxidase-Activating System.

Li D, Liang Y, Wang X, Wang L, Qi M, Yu Y, Luan Y - G3 (Bethesda) (2015)

Phylogenetic analysis of mdproPO unigenes. The amino acid sequences from eight unigenes of mdproPO in M. domestica and 21 proPO genes in other species including M. domestica (Md, 1), Sarcophaga bullata (Sb, 2), Drosophila melanogaster (Dm, 3), Anopheles gambiae (Ag, 2), Anopheles stephensi (As,1), Armigeres subalbatus (Ars, 3), Aedes aegypti (Aa, 1), Manduca sexta (Ms, 2), Bombyx mori (Bm, 2), Tenebrio molitor (Tm, 1), Holotrichia diomphalia (Hd, 2), and Eriocheir sinensis (Es, 1) were used to build the NJ phylogenetic tree by MEGA 5.0 with 1000 bootstraps. Six unigenes (circle) were clustered with Sb-proPO1 (disc) into group 1, and the other two unigenes (triangles) were clustered with Sb-proPO2 (triangle block) into group 2.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555219&req=5

fig6: Phylogenetic analysis of mdproPO unigenes. The amino acid sequences from eight unigenes of mdproPO in M. domestica and 21 proPO genes in other species including M. domestica (Md, 1), Sarcophaga bullata (Sb, 2), Drosophila melanogaster (Dm, 3), Anopheles gambiae (Ag, 2), Anopheles stephensi (As,1), Armigeres subalbatus (Ars, 3), Aedes aegypti (Aa, 1), Manduca sexta (Ms, 2), Bombyx mori (Bm, 2), Tenebrio molitor (Tm, 1), Holotrichia diomphalia (Hd, 2), and Eriocheir sinensis (Es, 1) were used to build the NJ phylogenetic tree by MEGA 5.0 with 1000 bootstraps. Six unigenes (circle) were clustered with Sb-proPO1 (disc) into group 1, and the other two unigenes (triangles) were clustered with Sb-proPO2 (triangle block) into group 2.
Mentions: The putative genes were provided mainly with reference to unigene annotation and unigene phylogenetic analysis (see Figure 5, Figure 6, Figure 7, Figure 8, and Figure S1).

Bottom Line: Of the 89,842 unigenes, based on a similarity search with known genes in other insects, 24 putative genes related to the proPO system were identified.Eight of the identified genes encoded for peptidoglycan recognition receptors, two encoded for prophenoloxidases, three encoded for prophenoloxidase-activating enzymes, and 11 encoded for serine proteinase inhibitors.Collectively, this study has provided the comprehensive transcriptomic data of an insect and some fundamental basis toward achieving understanding of the activation mechanisms and immune functions of the proPO system in M. domestica.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Department, The School of Biological Sciences and Biotechnology, University of Jinan, Jinan 250022, People's Republic of China chm_lidx@ujn.edu.cn.

No MeSH data available.


Related in: MedlinePlus