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Transcriptomic Analysis of Musca domestica to Reveal Key Genes of the Prophenoloxidase-Activating System.

Li D, Liang Y, Wang X, Wang L, Qi M, Yu Y, Luan Y - G3 (Bethesda) (2015)

Bottom Line: Of the 89,842 unigenes, based on a similarity search with known genes in other insects, 24 putative genes related to the proPO system were identified.Eight of the identified genes encoded for peptidoglycan recognition receptors, two encoded for prophenoloxidases, three encoded for prophenoloxidase-activating enzymes, and 11 encoded for serine proteinase inhibitors.Collectively, this study has provided the comprehensive transcriptomic data of an insect and some fundamental basis toward achieving understanding of the activation mechanisms and immune functions of the proPO system in M. domestica.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Department, The School of Biological Sciences and Biotechnology, University of Jinan, Jinan 250022, People's Republic of China chm_lidx@ujn.edu.cn.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic analysis of mdPGRP unigenes. The amino acid sequences from 21 mdPGRP unigenes of M. domestica and 14 PGRP genes of D. melanogaster including Dm-PGRP SAb (AHN59598), Dm-PGRP SB1 (AAF49420), Dm-PGRP SB2b (AGB94663), Dm-PGRP SC2 (AAF59051), Dm-PGRP SD (AAF50530), Dm-PGRP LAf (AAF50303), Dm-PGRP LBd (AFH06370), Dm-PGRP LCa (AAF50302), Dm-PGRP LCg (ACZ94669), Dm-PGRP LDc (AAO41277), Dm-PGRP LDd (ACL83248), Dm-PGRP LEa (AAF48519), Dm-PGRP LEb (AFH07408), and Dm-PGRP LF(AAF50301) were used to build the NJ phylogenetic tree by MEGA 5.0 with 1000 bootstraps. The 21 mdPGRP unigenes were clustered into eight groups, with each group containing at least one specific Dm-PGRP gene (marked diamond block).
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fig5: Phylogenetic analysis of mdPGRP unigenes. The amino acid sequences from 21 mdPGRP unigenes of M. domestica and 14 PGRP genes of D. melanogaster including Dm-PGRP SAb (AHN59598), Dm-PGRP SB1 (AAF49420), Dm-PGRP SB2b (AGB94663), Dm-PGRP SC2 (AAF59051), Dm-PGRP SD (AAF50530), Dm-PGRP LAf (AAF50303), Dm-PGRP LBd (AFH06370), Dm-PGRP LCa (AAF50302), Dm-PGRP LCg (ACZ94669), Dm-PGRP LDc (AAO41277), Dm-PGRP LDd (ACL83248), Dm-PGRP LEa (AAF48519), Dm-PGRP LEb (AFH07408), and Dm-PGRP LF(AAF50301) were used to build the NJ phylogenetic tree by MEGA 5.0 with 1000 bootstraps. The 21 mdPGRP unigenes were clustered into eight groups, with each group containing at least one specific Dm-PGRP gene (marked diamond block).

Mentions: The putative genes were provided mainly with reference to unigene annotation and unigene phylogenetic analysis (see Figure 5, Figure 6, Figure 7, Figure 8, and Figure S1).


Transcriptomic Analysis of Musca domestica to Reveal Key Genes of the Prophenoloxidase-Activating System.

Li D, Liang Y, Wang X, Wang L, Qi M, Yu Y, Luan Y - G3 (Bethesda) (2015)

Phylogenetic analysis of mdPGRP unigenes. The amino acid sequences from 21 mdPGRP unigenes of M. domestica and 14 PGRP genes of D. melanogaster including Dm-PGRP SAb (AHN59598), Dm-PGRP SB1 (AAF49420), Dm-PGRP SB2b (AGB94663), Dm-PGRP SC2 (AAF59051), Dm-PGRP SD (AAF50530), Dm-PGRP LAf (AAF50303), Dm-PGRP LBd (AFH06370), Dm-PGRP LCa (AAF50302), Dm-PGRP LCg (ACZ94669), Dm-PGRP LDc (AAO41277), Dm-PGRP LDd (ACL83248), Dm-PGRP LEa (AAF48519), Dm-PGRP LEb (AFH07408), and Dm-PGRP LF(AAF50301) were used to build the NJ phylogenetic tree by MEGA 5.0 with 1000 bootstraps. The 21 mdPGRP unigenes were clustered into eight groups, with each group containing at least one specific Dm-PGRP gene (marked diamond block).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555219&req=5

fig5: Phylogenetic analysis of mdPGRP unigenes. The amino acid sequences from 21 mdPGRP unigenes of M. domestica and 14 PGRP genes of D. melanogaster including Dm-PGRP SAb (AHN59598), Dm-PGRP SB1 (AAF49420), Dm-PGRP SB2b (AGB94663), Dm-PGRP SC2 (AAF59051), Dm-PGRP SD (AAF50530), Dm-PGRP LAf (AAF50303), Dm-PGRP LBd (AFH06370), Dm-PGRP LCa (AAF50302), Dm-PGRP LCg (ACZ94669), Dm-PGRP LDc (AAO41277), Dm-PGRP LDd (ACL83248), Dm-PGRP LEa (AAF48519), Dm-PGRP LEb (AFH07408), and Dm-PGRP LF(AAF50301) were used to build the NJ phylogenetic tree by MEGA 5.0 with 1000 bootstraps. The 21 mdPGRP unigenes were clustered into eight groups, with each group containing at least one specific Dm-PGRP gene (marked diamond block).
Mentions: The putative genes were provided mainly with reference to unigene annotation and unigene phylogenetic analysis (see Figure 5, Figure 6, Figure 7, Figure 8, and Figure S1).

Bottom Line: Of the 89,842 unigenes, based on a similarity search with known genes in other insects, 24 putative genes related to the proPO system were identified.Eight of the identified genes encoded for peptidoglycan recognition receptors, two encoded for prophenoloxidases, three encoded for prophenoloxidase-activating enzymes, and 11 encoded for serine proteinase inhibitors.Collectively, this study has provided the comprehensive transcriptomic data of an insect and some fundamental basis toward achieving understanding of the activation mechanisms and immune functions of the proPO system in M. domestica.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Department, The School of Biological Sciences and Biotechnology, University of Jinan, Jinan 250022, People's Republic of China chm_lidx@ujn.edu.cn.

No MeSH data available.


Related in: MedlinePlus