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Transcriptomic Analysis of Musca domestica to Reveal Key Genes of the Prophenoloxidase-Activating System.

Li D, Liang Y, Wang X, Wang L, Qi M, Yu Y, Luan Y - G3 (Bethesda) (2015)

Bottom Line: Of the 89,842 unigenes, based on a similarity search with known genes in other insects, 24 putative genes related to the proPO system were identified.Eight of the identified genes encoded for peptidoglycan recognition receptors, two encoded for prophenoloxidases, three encoded for prophenoloxidase-activating enzymes, and 11 encoded for serine proteinase inhibitors.Collectively, this study has provided the comprehensive transcriptomic data of an insect and some fundamental basis toward achieving understanding of the activation mechanisms and immune functions of the proPO system in M. domestica.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Department, The School of Biological Sciences and Biotechnology, University of Jinan, Jinan 250022, People's Republic of China chm_lidx@ujn.edu.cn.

No MeSH data available.


Related in: MedlinePlus

Length distribution of contigs, unigenes, and BLAST CDSs in M. domestica transcriptomic library.
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fig2: Length distribution of contigs, unigenes, and BLAST CDSs in M. domestica transcriptomic library.

Mentions: In current research, most contigs ranged from 200 nt to 3000 nt, and the lengths of unigenes ranged mostly from 300 nt to 3000 nt after the short unigenes (<300 nt) are removed. The numbers of contigs and unigenes decreased as their sequence sizes increased (Figure 2). To illustrate, the amount of unigenes longer than 500 nt was 36,190 (40.28%), whereas that longer than 1000 nt declined to 12,408 (13.8%). Similarly, 36,005 CDSs ranged from 201 nt to 500 nt, whereas only 247 CDSs were longer than 3 kb. It is worth mentioning that 391 contigs (>3 kb) are approximately 49-times that in published transcriptome (Liu et al. 2012).


Transcriptomic Analysis of Musca domestica to Reveal Key Genes of the Prophenoloxidase-Activating System.

Li D, Liang Y, Wang X, Wang L, Qi M, Yu Y, Luan Y - G3 (Bethesda) (2015)

Length distribution of contigs, unigenes, and BLAST CDSs in M. domestica transcriptomic library.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555219&req=5

fig2: Length distribution of contigs, unigenes, and BLAST CDSs in M. domestica transcriptomic library.
Mentions: In current research, most contigs ranged from 200 nt to 3000 nt, and the lengths of unigenes ranged mostly from 300 nt to 3000 nt after the short unigenes (<300 nt) are removed. The numbers of contigs and unigenes decreased as their sequence sizes increased (Figure 2). To illustrate, the amount of unigenes longer than 500 nt was 36,190 (40.28%), whereas that longer than 1000 nt declined to 12,408 (13.8%). Similarly, 36,005 CDSs ranged from 201 nt to 500 nt, whereas only 247 CDSs were longer than 3 kb. It is worth mentioning that 391 contigs (>3 kb) are approximately 49-times that in published transcriptome (Liu et al. 2012).

Bottom Line: Of the 89,842 unigenes, based on a similarity search with known genes in other insects, 24 putative genes related to the proPO system were identified.Eight of the identified genes encoded for peptidoglycan recognition receptors, two encoded for prophenoloxidases, three encoded for prophenoloxidase-activating enzymes, and 11 encoded for serine proteinase inhibitors.Collectively, this study has provided the comprehensive transcriptomic data of an insect and some fundamental basis toward achieving understanding of the activation mechanisms and immune functions of the proPO system in M. domestica.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Department, The School of Biological Sciences and Biotechnology, University of Jinan, Jinan 250022, People's Republic of China chm_lidx@ujn.edu.cn.

No MeSH data available.


Related in: MedlinePlus